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PubMed Journals Articles About "Kilohertz Photon Brain Imaging Awake Mice" RSS

08:40 EST 16th December 2019 | BioPortfolio

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Showing "Kilohertz photon brain imaging awake mice" PubMed Articles 1–25 of 21,000+

Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice.

Optical interrogation of voltage in deep brain locations with cellular resolution would be immensely useful for understanding how neuronal circuits process information. Here, we report ASAP3, a genetically encoded voltage indicator with 51% fluorescence modulation by physiological voltages, submillisecond activation kinetics, and full responsivity under two-photon excitation. We also introduce an ultrafast local volume excitation (ULoVE) method for kilohertz-rate two-photon sampling in vivo with increased ...


Voltage imaging with ANNINE dyes and two-photon microscopy of Purkinje dendrites in awake mice.

Voltage imaging is the next generation of functional imaging in neuroscience. It promises to resolve neuronal activity 10 to 100-times faster than calcium imaging and to report not only supra but also subthreshold activity on a single cell or even subcellular level. Lately, several different voltage sensors and imaging techniques were published which can achieve this. Here, we focus on a technique based on the synthetic pure electrochromic voltage-sensitive dyes ANNINE-6 and ANNINE-6plus and the excitation ...

Impact of atherosclerotic disease on cerebral microvasculature and tissue oxygenation in awake LDLR-/-hApoB+/+ transgenic mice.

We explore cortical microvasculature changes during the progression of atherosclerosis using young and old transgenic atherosclerotic (ATX) mice with thinned-skull cranial window. In awake animals, exploiting intrinsic signal optical imaging, Doppler optical coherence tomography, and two-photon microscopy, we investigate how the progression of atherosclerotic disease affects the morphology and function of cortical microvasculature as well as baseline cerebral tissue oxygenation. Results show that aged ATX m...


Sensory evoked fMRI paradigms in awake mice.

Mouse fMRI has become increasingly popular in the small animal imaging field. However, compared to the more commonly used rat fMRI, it is challenging for mouse fMRI to obtain robust and specific functional imaging results. In the meantime, in other neuroscience modalities such as optical imaging, functional recording in the awake mice is common and becoming standard. Therefore, in the current study we developed comprehensive setups and analysis pipeline for multi-sensory fMRI paradigms in the awake mice. Cu...

In vivo Deep-Brain Structural and Hemodynamic Multiphoton Microscopy Enabled by Quantum Dots.

Visualizing deep-brain vasculature and hemodynamics is key to understanding brain physiology and pathology. Among the various adopted imaging modalities, multiphoton microscopy (MPM) is well-known for its deep-brain structural and hemodynamic imaging capability. However, the largest imaging depth in MPM is limited by signal depletion in the deep brain. Here we demonstrate that quantum dots are an enabling material for significantly deeper structural and hemodynamic MPM in mouse brain in vivo. We characteriz...

Two- and three-photon absorption cross-section characterization for high-brightness, cell-specific multiphoton fluorescence brain imaging.

We demonstrate an accurate quantitative characterization of absolute two- and three-photon absorption (2PA and 3PA) action cross sections of a genetically encodable fluorescent marker Sypher3s. Both 2PA and 3PA action cross sections of this marker are found to be remarkably high, enabling high-brightness, cell-specific two- and three-photon fluorescence brain imaging. Brain imaging experiments on sliced samples of rat's cortical areas are presented to demonstrate these imaging modalities. The 2PA action cro...

Deep-brain 2-photon fluorescence microscopy in vivo excited at the 1700  nm window.

Here we demonstrate deep-brain 2-photon fluorescence microscopy in mouse in vivo, excited at the 1700 nm window. Through signal versus power measurement, we show that indocyanine green (ICG) is a promising 2-photon fluorescent dye excitable at the 1700 nm window. In order to excite ICG circulating in the vasculature in the deep brain, we employ a circular-polarization soliton self-frequency shift technique to generate energetic femtosecond pulses at 1617 nm. Combining the labeling and laser technologies,...

Stereotactic system for accurately targeting deep brain structures in awake head-fixed mice.

Deep brain nuclei, such as the amygdala, nucleus basalis, and locus coeruleus, play a crucial role in cognition and behavior. Nonetheless, acutely recording electrical activity from these structures in head-fixed awake rodents has been very challenging due to the fact that head-fixed preparations are not designed for stereotactic accuracy. We overcome this issue by designing the DeepTarget, a system for stereotactic head-fixation and recording, which allows for accurately directing recording electrodes or o...

Method for spike detection from microelectrode array recordings contaminated by artifacts of simultaneous two-photon imaging.

The simultaneous utilization of electrophysiological recordings and two-photon imaging allows the observation of neural activity in a high temporal and spatial resolution at the same time. The three dimensional monitoring of morphological features near the microelectrode array makes the observation more precise and complex. In vitro experiments were performed on mice neocortical slices expressing the GCaMP6 genetically encoded calcium indicator for monitoring the neural activity with two-photon microscopy a...

Atherosclerosis is associated with a decrease in cerebral microvascular blood flow and tissue oxygenation.

Chronic atherosclerosis may cause cerebral hypoperfusion and inadequate brain oxygenation, contributing to the progression of cognitive decline. In this study, we exploited two-photon phosphorescence lifetime microscopy to measure the absolute partial pressure of oxygen (PO2) in cortical tissue in both young and old LDLR-/-, hApoB100+/+ mice, spontaneously developing atherosclerosis with age. Capillary red-blood-cell (RBC) speed, flux, hematocrit and capillary diameter were also measured by two-photon imagi...

Surgical preparations, labeling strategies, and optical techniques for cell-resolved, in vivo imaging in the mouse spinal cord.

In vivo optical imaging has enabled detailed studies of cellular dynamics in the brain of rodents in both healthy and diseased states. Such studies were made possible by three advances: surgical preparations that give optical access to the brain; strategies for in vivo labeling of cells with structural and functional fluorescent indicators; and optical imaging techniques that are relatively insensitive to light scattering by tissue. In vivo imaging in the rodent spinal cord has lagged behind than that in th...

Awake brain surgery in children-review of the literature and state-of-the-art.

Awake brain surgery (ABS) is poorly reported in children as it is considered having limited indications due to age and neuropsychological aspects interfering with its feasibility and psychological outcome. The aim of this article is to review the current state-of-the-art of ABS in children and to offer an objective summary of the published literature on diversified outcome aspects of pediatric awake procedures.

Comparison of Optically-Derived Biomarkers in Healthy and Brain Tumor Tissue Under One- and Two-Photon Excitation.

The surgical outcome of brain tumor resection and needle biopsy is significantly correlated to the patient's prognosis. Brain tumor surgery is limited to resecting the solid portion of the tumor as current intraoperative imaging modalities are incapable of delineating infiltrative regions. For accurate delineation, in situ tissue interrogation at the submicron scale is warranted. Additionally, multimodal detection is required to remediate the genetically and molecularly heterogeneous nature of brain tumors,...

Two-Color Volumetric Imaging of Neuronal Activity of Cortical Columns.

To capture the emergent properties of neural circuits, high-speed volumetric imaging of neural activity at cellular resolution is needed. Here, we introduce wavelength multiplexing to perform fast volumetric two-photon imaging of cortical columns (>2,000 neurons in 10 planes at 10 vol/s), using two different calcium indicators, an electrically tunable lens and a spatial light modulator. We image the activity of neuronal populations from layers 2/3 to 5 of primary visual cortex from awake mice, finding a lac...

In vivo two-photon imaging/excited photothermal therapy strategy of a silver-nanohybrid.

A multi-functional nanohybrid (PyAnOH-Ag) with both a two-photon photothermal therapy (TP-PTT) effect and two-photon excited fluorescence (TPEF) imaging performance has been fabricated based on interfacial coordination interactions. The hybrid possesses a high two-photon absorption cross section (δTPA, 4638 GM) and detectable TPEF signals, which leads to excellent two-photon photothermal conversion. Upon irradiation at 840 nm, the temperature of the PyAnOH-Ag-PBS suspension reaches 42 °C in just 4 min, wh...

Tissue-Imaging of Glutathione-Specifical Naphthalimide-Cyanine Dye with Two-Photon and Near-Infrared Manners.

Molecular probes suitable for different fluorescence imaging technologies can meet the requirements of different scientific researches in biological applications. In this work, a naphthalimide-cyanine based sulfonamide was used to specifically visualize the glutathione of mouse tissues with a two-photon manner for the naphthalimide moiety and a near-infrared manner for the cyanine moiety, respectively. The results showed that this probe served as a dual-model tissue-imaging agent for the visualization of gl...

IMMERSING PATIENTS IN A VIRTUAL REALITY ENVIRONMENT FOR BRAIN MAPPING DURING AWAKE SURGERY. SAFETY STUDY.

Brain mapping by direct electrical stimulation (DES) during awake craniotomy is now a standard procedure which reduces the risk of permanent neurologic deficits. Virtual reality technology (VR) immerses the patient in a virtually controlled, interactive world, offering a unique opportunity to develop innovative tasks for peroperative mapping of complex cognitive functions. The objective of this prospective monocentric study was to evaluate the tolerance and safety of a virtual reality headset (VRH) and imme...

Propagations of spontaneous brain activity in awake rats.

Slow propagations of spontaneous brain activity have been reported in multiple species. However, systematical investigation of the organization of such brain activity is still lacking. In this study, we analyzed propagations of spontaneous brain activity using a reference library of characteristic resting-state functional connectivity (RSFC) patterns in awake rodents. We found that transitions through multiple distinct RSFC patterns were reproducible not only in transition sequences but also in transition t...

Single and population coding of taste in the gustatory cortex of awake mice.

Electrophysiological analysis has reveals much about the broad coding and neural ensemble dynamics that characterize gustatory cortical (GC) taste processing in awake rats, and about how these dynamics relate to behavior. With regard to mice, meanwhile, data concerning cortical taste coding have largely been restricted to imaging-a technique that reveals average levels of neural responsiveness, but that (currently) lacks the temporal sensitivity necessary for evaluation of fast response dynamics; furthermor...

Multi-band SWIFT enables quiet and artefact-free EEG-fMRI and awake fMRI studies in rat.

Functional magnetic resonance imaging (fMRI) studies in animal models provide invaluable information regarding normal and abnormal brain function, especially when combined with complementary stimulation and recording techniques. The echo planar imaging (EPI) pulse sequence is the most common choice for fMRI investigations, but it has several shortcomings. EPI is one of the loudest sequences and very prone to movement and susceptibility-induced artefacts, making it suboptimal for awake imaging. Additionally,...

Comparison of Awake and Asleep Deep Brain Stimulation for Parkinson's Disease: A Detailed Analysis Through Literature Review.

Deep brain stimulation (DBS) for Parkinson's disease (PD) has been applied to clinic for approximately 30 years. The goal of this review is to explore the similarities and differences between "awake" and "asleep" DBS techniques.

Fluorescent Imaging and Microscopy for Dynamic Processes in Rats.

The rat is a favored model organism to study physiological function in vivo. This is largely due to the fact that it has been used for decades and is often more comparable to corresponding human conditions (both normal and pathologic) than mice. Although the development of genetic manipulations in rats has been slower than in mice, recent advances of new genomic editing tools allow for the generation of targeted global and specific cell type mutations in different rat strains. The rat is an ideal model for ...

A mitochondria-targeted two-photon fluorescent probe for sensing and imaging pH changes in living cells.

A novel two-photon pH probe, 3-benzimidazole-7-hydroxycoumarin (BHC), was designed and synthesized based on the structures of hydroxycoumarin and benzimidazole. BHC showed good linearity in the pH ranges of 3.30-5.40 (pKa = 4.20) and 6.50-8.30 (pKa = 7.20) at a maximum emission wavelength of 480 nm. BHC in acidic and alkaline media could be distinguished by an obvious spectral shift of the maximum absorption wavelength from 390 nm to 420 nm. In addition, BHC was well localized to mitochondria ...

Novel Tc-2-arylimidazo2,1-bbenzothiazole derivatives as SPECT imaging agents for amyloid-β plaques.

Six novel 2-arylimidazo[2,1-b]benzothiazole (IBT) derivatives were synthesized as potential tridentate radiotracers for AD imaging purposes. Two of these ligands (6a,b) were successfully labeled with Tc radionuclide at high radiochemical purity using fac-[Tc(CO)(HO)] synthon. [Tc]7a and [Tc]7b were evaluated as single photon emission computed tomography (SPECT) imaging agents for Aβ plaque in AD. [Tc]7a and [Tc]7b exhibited suitable affinity toward Aβ aggregates with IC values of 33.2 and 102.5 nM, resp...

Nuclear-targeted of TAT peptide-conjugated carbon dots for both one-and two-photon fluorescence imaging.

Carbon dots (CDs) with both one- and two-photon fluorescence have attracted much attention in the field of bioimaging. In this study, we reported a strategy for the design of peptide-conjugated CDs for cellular nuclear-targeted imaging. The CDs were prepared from amino acid and formic acid via an one-step hydrothermal synthesis method, and then modified with trans-activator of transcription (TAT) peptide for both one- and two- photon nuclear-targeted fluorescence imaging. The CDs derived from tryptophan (Tr...


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