PubMed Journals Articles About "Mass Spectrometry Help Limit Reproducibility Problems Http Dlvr" RSS

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Showing "Mass Spectrometry Help Limit Reproducibility Problems http dlvr" PubMed Articles 1–25 of 12,000+

Accessible and reproducible mass spectrometry imaging data analysis in Galaxy.

Mass spectrometry imaging is increasingly used in biological and translational research because it has the ability to determine the spatial distribution of hundreds of analytes in a sample. Being at the interface of proteomics/metabolomics and imaging, the acquired datasets are large and complex and often analyzed with proprietary software or in-house scripts, which hinders reproducibility. Open source software solutions that enable reproducible data analysis often require programming skills and are therefo...

Determination of multiple pesticide residues in teas by gas chromatography with accurate time-of-flight mass spectrometry.

In this work, gas chromatography tandem with electron ionization and full-scan high-resolution mass spectrometry with a time-of-flight mass analyzer was evaluated for analyzing pesticide residues in teas. The relevant aspects for mass spectrometry analysis, including the resolution and mass accuracy, acquisition rate, temperature of ion source, were investigated. Under acquisition condition in 2-GHz extended dynamic range mode, accurate mass spectral library including 184 gas chromatography detectable pesti...

Monitoring recombinant protein expression in bacteria by rapid evaporative ionisation mass spectrometry.

There is increasing interest in methods of direct analysis mass spectrometry that bypass complex sample preparation steps.

Improvement of hair testing for Ethylglucuronide by supported liquid extraction and ultra-high performance liquid chromatography tandem mass spectrometry.

To improve the reproducibility, suitability and speed of hair testing for Ethylglucuronide (EtG), an ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) method was developed and validated together with a supported liquid extraction (SLE) EtG from the keratin matrix. EtG was analyzed using reversed phase chromatography with gradient elution and detection with tandem mass spectrometry operated in multiple reaction monitoring (MRM) mode via negative electrospray ioniz...

Collagen analysis with mass spectrometry.

Mass spectrometry-based techniques can be applied to investigate collagen with respect to identification, quantification, supramolecular organization, and various post-translational modifications. The continuous interest in collagen research has led to a shift from techniques to analyze the physical characteristics of collagen to methods to study collagen abundance and modifications. In this review, we illustrate the potential of mass spectrometry for in-depth analyses of collagen.

Identification of novel biomarkers of hepatocellular carcinoma by a high definition mass spectrometry; UHPLC-QTOF/MS and DESI-MSI.

Hepatocellular carcinoma (HCC) is a highly malignant disease for which the development of prospective or prognostic biomarkers is urgently required. Although metabolomics is widely used for biomarker discovery, there are some bottlenecks regarding the comprehensiveness of detected features, reproducibility of methods, and identification of metabolites. In addition, the information on localization of metabolites in the tumor tissue is needed for functional analysis. Here, we developed a wide-polarity global ...

Characterisation of phenylethanoid glycosides by multiple-stage mass spectrometry.

Although phenylethanoid glycosides (PhGs) occur widely in plants, their characterisation by liquid chromatography/mass spectrometry (LC/MS) is less well studied than other phenolic glycosides such as flavonoid glycosides. The multiple-stage mass spectrometry (MS ) experiments required to improve the annotation of common verbascoside-type PhGs are described here.

Towards a generic method for ion chromatography-mass spectrometry of low molecular weight amines in pharmaceutical drug discovery and development.

Low molecular weight amines are encountered in pharmaceutical analysis e.g. as reactants in chemical syntheses, but are challenging to analyse by ultra-high-performance liquid chromatography-mass spectrometry (UHPLC/MS) due to their high polarity causing poor retention. Ion chromatography-MS (IC-MS) is an emerging technique for polar molecule analysis that offers better separation. A generic IC-MS method would overcome problems associated with using UHPLC/MS in drug discovery and development environments.

Nucleobase Derivatives Induce In-Source Decay of Oligonucleotides as New MALDI Matrices.

For quality control of oligonucleotide therapeutics, accurate and efficient structural characterization using mass spectrometry techniques, such as liquid chromatography-mass spectrometry (LC-MS) and matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF MS) is essential. In MALDI MS analysis, matrix selection is critical and a new matrix could enable more efficient and rapid structural analysis.

Electrospray Ionization Enters the Final Frontier: Mass Spectrometry's Role in Understanding Electrospray Thrusters and Their Plumes.

Electrospray thrusters using ionic liquid-based propellants are quickly gaining popularity in spacecraft design. Mass spectrometry is especially well-suited to provide important knowledge on the fundamentals of how these systems work and on evaluating their efficiencies and impacts, given that the operating principles of electrospray thrusters closely mimics the mass spectrometry experiment-in both ions are generated by electrospray and then enter a vacuum. Here, electrospray thruster technology and ionic l...

Improved MALDI-MS method for the highly sensitive and reproducible detection of biomarker peaks for the proteotyping of Salmonella serotypes.

The rapid identification and classification of pathogenic microorganisms, including Salmonella enterica, is important for the surveillance and prevention of foodborne diseases. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) has been shown to be an effective tool for the rapid identification of microorganisms. In a previous report, a mass database consisting of twelve biomarker proteins, S8, L15, L17, L21, L25, S7, superoxide dismutase (SodA), peptidylprolyl cis-tr...

Characterizing chain-end structures formed during initiation reactions of radical polymerization for MMA-St-BA terpolymer using pyrolysis-gas chromatography-atmospheric pressure chemical ionization-high resolution time-of-flight mass spectrometry.

Analyzing polymer end groups using pyrolysis (Py) gas chromatography-mass spectrometry (GC/MS) in multi-component polymer samples is not an easy task because of the insufficient sensitivity, selectivity, and mass resolution of conventional Py-GC/MS systems.

Feasibility of microwave-induced combustion combined with inductively coupled plasma mass spectrometry for bromine and iodine determination in human nail.

RATIONALE Bromine and iodine have important physiological functions and they can also result in physiological problems when in inadequate concentration. Their mobility assessment in human organism through biological samples analysis may help to clarify some doubts related to metabolic routes, which are still not well elucidated. In this context, a suitable analytical method for this purpose should be developed.

Postsynthesis of zwitterionic hydrophilic composites for enhanced enrichment of N-linked glycopeptides from human serum.

Proteins glycosylation plays an important role in life activities, but the concentration of naturally occurring glycopeptides are usually relatively low and glycosylation has microfacies heterogeneity, so direct mass spectrometry is not feasible. Therefore, how to selective enrich glycopeptides before mass spectrometry has turn into the urgent problem to be resolved.

Dual strategy for reduced signal suppression effects in MALDI mass spectrometry imaging.

Development of a Relative Quantification Method for IR-MALDESI Mass Spectrometry Imaging of Arabidopsis Root Seedlings.

Mass spectrometry imaging of young seedlings is an invaluable tool in understanding how mutations affect metabolite accumulation in plant development. However, due numerous biological considerations, established methods for the relative quantification of analytes using IR-MALDESI mass spectrometry imaging are not viable options. In this study, we report a method for quantification of auxin-related compounds using stable-isotope-labelled (SIL) indole-3-acetic acid (IAA) doped into agarose substrate.

Lys-Sequencer: An Algorithm for de novo Sequencing of Peptides by Paired Single Residue-Transposed Lys-C and Lys-N Digestion Coupled with High Resolution Mass Spectrometry.

Database-dependent identification of proteins by mass spectrometry is well established, but has limitations when there are novel proteins, mutations, splice variants, and post-translational modifications (PTMs) not available in the established reference database. De novo sequencing as a database-independent approach could address these limitations by deducing peptide sequences directly from experimental tandem mass spectrometry spectra, while concomitantly yielding residue-by-residue confidence metrics.

Preparation of Samples for a Mass Spectrometry-Based Method to Identify Allergenic Proteins.

Western blotting is an immunological technique that has been combined with mass spectrometry analysis, to create a high-throughput method for protein identification. Western blotting using serum allows us to identify a protein within an allergenic extract that specifically binds to a serum antibody, immunoglobulin E. This specific IgE binding protein can then be detected with a highly sensitive chemiluminescence detection substrate.Proteins detected by western blotting can be analyzed by mass spectrometry f...

Rapid and accurate diagnosis of hemoglobinopathy and β-thalassemia by ultrahigh-resolution mass spectrometry and tandem mass spectrometry from blood: review of a benchmark study.

Vibrating tip spray ionization mass spectrometry for direct sample analysis.

In this work, a vibrating tip spray ionization source was developed for direct mass spectrometric analysis of raw samples under voltage-free condition. A solid tip was mounted on a vibrator, and the solid tip was placed on the front of MS inlet. Liquid, viscous and bulk solid samples could be directly loaded on the tip-end surface, and then a drop of solvent at microliter level was subsequently loaded on the tip for dissolution and extraction of analytes, and a vibrator was then started to atomize and ioniz...

Reproducibility of biomarker identifications from mass spectrometry proteomic data in cancer studies.

Reproducibility of disease signatures and clinical biomarkers in multi-omics disease analysis has been a key challenge due to a multitude of factors. The heterogeneity of the limited sample, various biological factors such as environmental confounders, and the inherent experimental and technical noises, compounded with the inadequacy of statistical tools, can lead to the misinterpretation of results, and subsequently very different biology. In this paper, we investigate the biomarker reproducibility issues,...

Online porous graphic carbon chromatography coupled with tandem mass spectrometry for post-translational modification analysis.

Porous graphic carbon chromatography (PGC) has different mechanism in retention of tryptic peptides compared with reversed phase chromatography and in this study it was shown that coupling PGC with tandem mass spectrometry offer advantages for the quantitation of phosphorylation stoichiometry and characterization of site-specific glycosylation.

Unexpected cytosine-AuCl interaction under electrospray ionization mass spectrometry conditions-Formation of cytosine-Au(I) complexes.

A complex matrix characterization approach, applied to cigarette smoke, that integrates multiple analytical methods and compound identification strategies for non-targeted liquid chromatography with high-resolution mass spectrometry.

Preliminary investigation of aluminium fluoride complexes in aqueous solutions with CE/ESI-MS and CE/ICP-MS.

It is crucial to identify and confirm the original species of aluminium ions (Al ) dissolved in water, since they behave differently. Depending on their species, the toxicity differs. Capillary electrophoresis (CE) coupled with electrospray ionization mass spectrometry (ESI-MS) and CE coupled with inductively coupled plasma mass spectrometry (CE/ICP-MS) were explored to identify and determine simple systems of Al species solutions at pH 3.0.

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