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PubMed Journals Articles About "More Than Coli Illnesses Linked Romaine Lettuce" RSS

03:50 EST 20th February 2020 | BioPortfolio

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Showing "More Than Coli Illnesses Linked Romaine Lettuce" PubMed Articles 1–25 of 5,700+

Shiga Toxin-Producing (STEC) O157:H7 and Romaine Lettuce: Source Labeling, Prevention, and Business.

From May to November most romaine lettuce shipments in the United States come from California's Central Coast region, whereas from December to April most come from the Yuma, Arizona, region. During 2017-2018, the 3 outbreaks of Shiga toxin-producing (STEC) O157:H7 in US romaine lettuce all occurred at the tail end of a region's production season. During the fall 2018 outbreak, the US Food and Drug Administration (FDA) recommended that suppliers begin labeling romaine packaging so that consumers can identif...


Culturable bacteria resident on lettuce might contribute to accumulation of human noroviruses.

Human noroviruses (HuNoVs) are the primary non-bacterial pathogens causing acute gastroenteritis worldwide. Attachment and invasion of HuNoVs are thought to involve histo-blood group antigens (HBGAs). Romaine lettuce, which is usually consumed raw, is a common food-related vehicle for HuNoVs transmission. This study investigated the possibility that bacteria resident on the surface of lettuce leaves contribute to norovirus adherence to this food. To test this hypothesis, bacteria were isolated from romaine ...

Modeling of Free Chlorine Consumption and Escherichia coli O157:H7 Cross-Contamination During Fresh-Cut Produce Wash Cycles.

Controlling the free chlorine (FC) availability in wash water during sanitization of fresh produce enhances our ability to reduce microbial levels and prevent cross-contamination. However, maintaining an ideal concentration of FC that could prevent the risk of contamination within the wash system is still a technical challenge in the industry, indicating the need to better understand wash water chemistry dynamics. Using bench-scale experiments and modeling approaches, we developed a comprehensive mathematic...


Microbiome convergence following sanitizer treatment and identification of sanitizer resistant species from spinach and lettuce rinse water.

Fresh produce, as a known or suspected source of multiple foodborne outbreaks, harbors large populations of diverse microorganisms, which are partially released into wash water during processing. However, the dynamics of bacterial communities in wash water during produce processing is poorly understood. In this study, we investigated the effect of chlorine (FC) and peracetic acid (PAA) on the microbiome dynamics in spinach and romaine lettuce rinse water. Treatments with increasing concentrations of sanitiz...

Optimization of Bacterial Concentration by Filtration for Rapid Detection of Foodborne Escherichia coli O157:H7 Using Real-Time PCR Without Microbial Culture Enrichment.

Escherichia coli O157:H7 is an important foodborne pathogen and has been implicated in numerous food poisoning outbreaks worldwide. Although several microbiological and molecular methods have been developed to detect E. coli O157:H7, the difficulty to rapidly detect low levels of the foodborne bacteria persists. Here, the optimization of a filtration technique to concentrate and rapidly detect E. coli O157:H7 was conducted. Using homogenates prepared from freshly cut lettuce and cabbage samples, the E. coli...

Bactericidal Efficacy of a Two-Dimensional Array of Integrated, Coaxial, Microhollow, Dielectric Barrier Discharge Plasma Against Serovar Heidelberg.

We studied the efficacy of cold atmospheric-pressure plasma (CAP), generated by a two-dimensional array of integrated, coaxial, microhollow, dielectric barrier discharge plasma, against serovar Heidelberg (SH) on stainless steel, romaine lettuce, and chicken breast. Exposure of SH to CAP on a dry stainless steel surface had low bactericidal efficacy; only 2.5 log colony-forming units (CFUs) were inactivated after 10 min of exposure. On the other hand, the presence of moisture led to decontamination of ...

Effects of polyethylene microplastic on the phytotoxicity of di-n-butyl phthalate in lettuce (Lactuca sativa L. var. ramosa Hort).

The increase in the proportion of microplastics in the environment has intensified the interest in phthalate and microplastic contamination in recent years. In this study, we investigated the response of photosynthetic parameters and the antioxidant system of lettuce to di-n-butyl phthalate (DBP) stress and exposure to various concentrations of microplastic polyethylene (MP) for different durations (14 d and 28 d). Lettuce growth, photosynthetic parameters, and chlorophyll content were reduced significant...

Arsenic transformation mediated by gut microbiota affects the fecundity of Caenorhabditis elegans.

Arsenic biotransformation has been discovered in guts of soil invertebrates. Reproduction of invertebrates is sensitive to arsenic contamination in soils. However, little is known about the impact of gut microbe-mediated arsenic biotransformation on the fecundity of invertebrates. Here, Caenorhabditis elegans was firstly pre-fed with Escherichia coli BL21 possessing the capability of reducing arsenate [As(V)] or BL21M having the ability to reduce As(V) and methylate arsenite [As(III)], then inoculated worms...

Left In The Dust: Helping Underreporting of Workplace Illnesses.

Workplace illnesses can be difficult for physicians and patients because some take years to develop and frequently are masked or mimicked by other illnesses. Such a disease may not show itself until decades after the patient has left the job that caused the problem. By then, the illness may be so far along that little can be done. That time lag between exposure and illness is just one of several difficulties Texas physicians face in tackling workplace illnesses. Because Texas does not have a federally-appro...

Effects of Zn in sludge-derived biochar on Cd immobilization and biological uptake by lettuce.

Considering the high Zn content of municipal sewage sludge and its competition with Cd during plant uptake due to their similar properties, the presence of Zn in sludge-derived biochar (SDBC) may affect Cd immobilization and uptake by plants. To confirm this, SDBC samples with different Zn contents were prepared and characterized. Their Cd immobilization behavior was studied by conducting batch sorption experiments, and their effects on Cd uptake by lettuce were explored by conducting hydroponic experiments...

Glutathione biosynthesis plays an important role in microcystin-LR depuration in lettuce and spinach.

Irrigation of crop plants with microcystins (MCs) contaminated water could be a threat to human health via bioaccumulation. Despite the fact MCs bioaccumulation in crop plants is well documented, MCs depuration, as well as the mechanism involved remains unclear. The objectives of the present study were to investigate the bioaccumulation and depuration of microcystin-LR (MC-LR) in lettuce (Lactuca sativa L.) and spinach (Spinacia oleracea L.), as well as to explore the role of glutathione (GSH) biosynthesis ...

Mechanistic study on uptake and transport of pharmaceuticals in lettuce from water.

The dissemination of pharmaceuticals in agroecosystems originating from land application of animal manure/sewage sludge and irrigation with treated wastewater in agricultural production has raised concern about the accumulation of pharmaceuticals in food products. The pathways of pharmaceutical entries via plant roots, transport to upper fractions, and the factors influencing these processes have yet been systematically elucidated, thus impeding the development of effective measures to mitigate pharmaceutic...

Pathogenic Escherichia coli (E. coli) detection through tuned nanoparticles enhancement study.

This study aims to detect pathogenic Escherichia coli (E. coli) bacteria using non-destructive fluorescence microscopy and micro-Raman spectroscopy.

Dynamic changes in the physicochemical properties of fresh-cut produce wash water as impacted by commodity type and processing conditions.

Organic materials in fresh-cut produce wash water deplete free chlorine that is required to prevent pathogen survival and cross-contamination. This research evaluated water quality parameters frequently used to describe organic load for their fitness to predict chlorine demand (CLD) and chemical oxygen demand (COD), which are major needs identified by the industry-led produce food safety taskforce. Batches of romaine lettuce, iceberg lettuce, or carrot of different cut sizes and shapes were washed in 40 lit...

Dissipation Profiles of Tristyrylphenol Ethoxylate Homologues in Lettuce under Greenhouse and Field Conditions.

Tristyrylphenol ethoxylates (TSPEOs) have been increasingly used in pesticide formulations as inert ingredients in China, but little information exists on the dissipation behavior of TSPEOs in foodstuffs. In this work, a rapid method for measuring TSPEO homologues in lettuce using QuEChERS and HPLC-MS/MS was established. This method was used to study the dissipation and distribution profiles of TSPEOs in lettuce. TSPEO homologues degraded rapidly under greenhouse and field conditions, with half-lives of 2.1...

Effect of Biochar on the Enantioselective Soil Dissipation and Lettuce Uptake and Translocation of the Chiral Pesticide Metalaxyl in a Contaminated Soil.

Enantioselectivity is usually ignored when assessing potential biochar-based methods of redressing pesticide contamination of soils. In this study, the effect of woodchip biochar (WBC) on the enantioselective dissipation of metalaxyl in soil and its uptake and translocation by lettuce were investigated. S-metalaxyl (T1/2=29.8 days) dissipated more quickly than R-metalaxyl (T1/2=36.4 days) in unamended soil. The addition of WBC to the soil decreased the dissipation rate and the enantioselectivity of metalaxy...

Variability in growth responses of non-O157 EHEC isolates in leafy vegetables, sprouted seed and soil extracts occurs at the isolate level.

Foods of plant origin are recognised as a major source of food-borne pathogens, in particular for Shigatoxigenic Escherichia coli (STEC). Most work for STEC and plant-based fresh produce has focused on the most prevalent outbreak serogroup, O157. However, non-O157 STEC are an emerging hazard, and as such it is important to characterise aspects within this group that reflect their ability to colonise alternative hosts and habitats relevant to horticultural production. Growth kinetics were quantified for a di...

Analysis of virulence potential of Escherichia coli O145 isolated from cattle feces and hide samples based on whole genome sequencing.

Escherichia coli O145 serogroup is one of the big six non-O157 Shiga toxin producing E. coli (STEC) that causes foodborne illnesses in the United States and other countries. Cattle are a major reservoir of STEC, which harbor them in their hindgut and shed in the feces. Cattle feces is the main source of hide and subsequent carcass contaminations during harvest leading to foodborne illnesses in humans. The objective of our study was to determine the virulence potential of STEC O145 strains isolated from catt...

Temporal influence of different antibiotics onto the inhibition of Escherichia coli bacterium grown in different media.

Escherichia coli (E. coli) is a Gram-negative bacterium commonly found in the lower intestine of warm-blooded organisms, including humans. Although the majority of the strains are considerably harmless, some serotypes are pathogenic, frequently causing diarrhea and other illnesses outside the intestinal tract. The standard antidote against bacteria is the use of antibiotics. Depending on their type, the antibiotics have various mechanisms of action on bacteria. Moreover, in case of in-vitro cultivation of b...

Microfluidic smartphone quantitation of Escherichia coli in synthetic urine.

In spite of the clinical need, there is a major gap in rapid diagnostics for identification and quantitation of E. coli and other pathogens, also regarded as the biggest bottleneck in the fight against the spread of antimicrobial resistant bacterial strains. This study reports for the first time an optical, smartphone-based microfluidic fluorescence sandwich immunoassay capable of quantifying E. coli in buffer and synthetic urine in less than 25 min without sample preparation nor concentration. A limit of...

Inactivated recombinant Escherichia coli as a candidate vaccine against Clostridium perfringens alpha toxin in sheep.

Clostridium perfringens type A is the causative agent of gas gangrene and gastroenteric ("yellow lamb disease") disease in ruminants, with C. perfringens alpha toxin (CPA) being the main virulence factor in the pathogenesis of these illnesses. In the present study, we have developed recombinant Escherichia coli bacterins expressing rCPA and used it to vaccinate rabbits and sheep. Doses of up to 200 μg of rCPA used for inoculation, induced 13,82 IU.mL of neutralizing antitoxin in rabbits, which is three tim...

Agronomic performance of lettuce cultivars submitted to different irrigation depths.

The use of cultivars adapted to the climate and soil conditions associated with adequate irrigation supply maximizes lettuce agronomic performance. The aim of this work was to evaluate the agronomic performance of four lettuce cultivars submitted to five different drip irrigation depths under a protected environment in Viçosa-MG, Brazil. A randomized block design was applied in a split plot scheme with four replications, and several agronomic characteristics were evaluated by analysis of variance, Tukey ra...

Emergence and transmission of New Delhi metallo-beta-lactamase-5-producing Escherichia coli Sequence Type 361 in a Tertiary Hospital in South Korea.

The emergence of carbapenem-resistant Escherichia coli (E coli) is a serious global health threat, but little is known about carbapenemase-producing E coli in Daejeon, South Korea. The aim of this study was to investigate characteristics of thirteen carbapenem-resistant E coli isolates in a tertiary hospital.

Identifying geographic origins of the Escherichia coli isolates from food by a method based on single-nucleotide polymorphisms.

E.coli is an important foodborne pathogen. Rapid and robust tracking of the source of E. coli is the key step to control foodborne infections.

A ten-year review of ESBL and non-ESBL Escherichia coli bloodstream infections among children at a tertiary referral hospital in South Africa.

There are few studies describing Escherichia coli (E. coli) bloodstream infection (BSI) among children in Africa, yet E.coli is increasing in importance as a cause of antibiotic resistant infection in paediatric settings.


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