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PubMed Journals Articles About "Proteome Systems Limited" RSS

10:52 EDT 20th July 2018 | BioPortfolio

Proteome Systems Limited PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest Proteome Systems Limited articles that have been published worldwide.

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We have published hundreds of Proteome Systems Limited news stories on BioPortfolio along with dozens of Proteome Systems Limited Clinical Trials and PubMed Articles about Proteome Systems Limited for you to read. In addition to the medical data, news and clinical trials, BioPortfolio also has a large collection of Proteome Systems Limited Companies in our database. You can also find out about relevant Proteome Systems Limited Drugs and Medications on this site too.

Showing "Proteome Systems Limited" PubMed Articles 1–25 of 8,500+

Merged Map of the Yeast Proteome.

A comprehensive reference map of protein abundances in budding yeast is generated by combining the 21 largest quantitative proteome datasets currently available for this model organism.


Unification of Protein Abundance Datasets Yields a Quantitative Saccharomyces cerevisiae Proteome.

Protein activity is the ultimate arbiter of function in most cellular pathways, and protein concentration is fundamentally connected to protein action. While the proteome of yeast has been subjected to the most comprehensive analysis of any eukaryote, existing datasets are difficult to compare, and there is no consensus abundance value for each protein. We evaluated 21 quantitative analyses of the S. cerevisiae proteome, normalizing and converting all measurements of protein abundance into the intuitive...

Abiotic stress-induced chloroplast proteome remodelling: a mechanistic overview.

The chloroplast houses photosynthesis in all green plants, and is therefore of fundamental importance to the viability and productivity of plants, ecosystems, and agriculture. Chloroplasts are, however, extremely vulnerable to environmental stress, on account of the inherent volatility of oxygenic photosynthesis. To counteract this sensitivity, sophisticated systems of chloroplast stress acclimation have evolved, and many of these involve broad proteome changes. Here, we provide an overview of the interlock...


Thermal proteome profiling in bacteria: probing protein state .

Increasing antibiotic resistance urges for new technologies for studying microbes and antimicrobial mechanism of action. We adapted thermal proteome profiling (TPP) to probe the thermostability of proteins had a more thermostable proteome than human cells, with protein thermostability depending on subcellular location-forming a high-to-low gradient from the cell surface to the cytoplasm. While subunits of protein complexes residing in one compartment melted similarly, protein complexes spanning compartmen...

Proteome Heterogeneity in Colorectal Cancer.

Tumour heterogeneity is an important feature of colorectal cancer (CRC) manifested by dynamic changes in gene expression, protein expression, and availability of different tumour subtypes. Recent publications in the past ten years have revealed proteome heterogeneity between different colorectal tumours and within the same tumour site. This paper reviews recent research works on the proteome heterogeneity in CRC, which includes the heterogeneity within a single tumour (intratumour heterogeneity), between di...

Application of targeted mass spectrometry in bottom-up proteomics for systems biology research.

The enormous diversity of proteoforms produces tremendous complexity within cellular proteomes, facilitates intricate networks of molecular interactions, and constitutes a formidable analytical challenge for biomedical researchers. Currently, quantitative whole-proteome profiling often relies on non-targeted liquid chromatography-mass spectrometry (LC-MS), which samples proteoforms broadly, but can suffer from lower accuracy, sensitivity, and reproducibility compared with targeted LC-MS. Recent advances in ...

A Host Proteome Atlas of Streptococcus pyogenes Infection.

Multiplex quantitative proteomics analysis of mice infected with Group A Streptococcus reveals organ-specific biomarkers of infection.

Analysis of PNGase F-resistant N-glycopeptides using SugarQb for Proteome Discoverer 2.1 reveals cryptic substrate specificities.

SugarQb (www.imba.oeaw.ac.at/sugarqb) is a freely available collection of computational tools for the automated identification of intact glycopeptides from high-resolution HCD MS/MS data-sets in the Proteome Discoverer environment. We report the migration of SugarQb to the latest and free version of Proteome Discoverer 2.1, and apply it to the analysis of PNGase F-resistant N-glycopeptides from mouse embryonic stem cells. The analysis of intact glycopeptides highlights unexpected technical limitations to PN...

PROTEINS AND PROTEOFORMS: New Separation Challenges.

The ancient Greeks attributed life to Mother Nature; complaining that she was fond of hiding the workings of her creations. Although we no longer attribute the mysteries of life to Mother Nature, multiple facets of living systems still evade us. The complexity of the human proteome and proteoform families are examples. Until recently we believed that each of the ~20,000 protein-coding genes in humans produced a single protein ; assuming this small number of proteins was sufficient to sustain life. Wrong! We...

Integrated platform with combination of on-line protein digestion, isotope dimethyl labeling and multidimensional peptide separation for high-throughput proteome quantification.

In recent years, various enzymatic microreactors and on-line enzyme digestion strategies have been widely applied in high throughput proteome analysis. However, the incomplete and irreproducible digestion would introduce some unexpected variations in comparative proteome quantification when the samples are digested and then chemically isotope labeled in different aliquots. To address these problems, we developed an integrated platform for high throughput proteome quantification with combination of on-line l...

Extending single molecule imaging to proteome analysis by quantitation of fluorescent labeling homogeneity in complex protein samples.

Fluorescence-based electrophoresis has been widely used for proteome analysis, in which every protein species in cells are labeled with a fluorescent dye, separated by electric migration and are quantified using fluorescence detection. The ultimate limit of sensitivity for this approach could be reached by single-molecule fluorescence imaging and counting individual proteins, requiring exhaustive fluorescent labeling of proteins across molecular populations and species. However, it remains unclear how homog...

Proteome-based systems biology in chronic pain.

Chronic pain represents a major medical challenge in the 21 century. Enormous efforts have been invested towards deciphering the complexity of chronic pain from different angles (molecular, physiological, psychosocial, and behavioral) in both preclinical and clinical settings. While progress has been made, our understanding of the underlying mechanisms of chronic pain remains insufficient. Consequently, chronic pain treatment is often inadequate. It lacks efficacy in most patients and is associated with det...

Rapid remodeling of the host epithelial cell proteome by the listeriolysin O pore-forming toxin.

Bacterial pathogens use various strategies to interfere with host cell functions. Among these strategies, bacteria modulate host gene transcription, thereby modifying the set of proteins synthetized by the infected cell. Bacteria can also target pre-existing host proteins and modulate their post-translational modifications or trigger their degradation. Analysis of protein levels variations in host cells during infection allows to integrate both transcriptional and post-transcriptional regulations induced by...

Proteome Profiling of Developing Murine Lens Through Mass Spectrometry.

We previously completed a comprehensive profile of the mouse lens transcriptome. Here, we investigate the proteome of the mouse lens through mass spectrometry-based protein sequencing at the same embryonic and postnatal time points.

Myeloperoxidase mediated HDL oxidation and HDL proteome changes do not contribute to dysfunctional HDL in Chinese subjects with coronary artery disease.

High density lipoprotein (HDL) cholesterol levels and cholesterol efflux capacity (CEC) are inversely correlated with coronary artery disease (CAD) risk. Myeloperoxidase (MPO) derived oxidants and HDL proteome changes are implicated in HDL dysfunction in subjects with CAD in the United States; however, the effect of MPO on HDL function and HDL proteome in ethnic Chinese population is unknown. We recruited four matched ethnic Chinese groups (20 patients each): subjects with 1) low HDL levels (HDL levels in m...

The Human Eye Proteome Project: Updates on an Emerging Proteome.

The human eye is a complex organ consisting of multiple compartments with unique and specialized properties that reflect their varied functions. Although there have been advancements in ocular imaging and therapeutics over the past decade, the pathogenesis of many common eye diseases remains poorly understood. Proteomics is an invaluable tool to gain insight into pathogenesis, diagnosis, and treatment of eye diseases. By 2013, when the Human Eye Proteome Project (also known as the EyeOme) was founded, there...

Curcumin exerts its antitumor effects in a context dependent fashion.

Proteome profiling profoundly contributes to the understanding of cell response mechanisms to drug actions. Such knowledge may become a key to improve personalized medicine. In the present study, the effects of the natural remedy curcumin on breast cancer model systems were investigated. MCF-7, ZR-75-1 and TGF-β1 pretreated fibroblasts, mimicking cancer-associated fibroblasts (CAFs), were treated independently as well as in tumor cell/CAF co-cultures. Remarkably, co-culturing with CAF-like cells (CLCs) ind...

Inventory of proteoforms as a current challenge of proteomics: Some technical aspects.

The main intricacy in the human proteome is that it is tremendously complex and composed from diverse and heterogeneous gene products. These products are called protein species or proteoforms and are the smallest units of the proteome. In pursuit of the comprehensive profiling of the human proteome, significant advances in the technology of so called "Top-Down" mass spectrometry based proteomics, have been made. However, the scale of performance of this approach is still far behind the "Bottom-Up", peptide-...

The Sarda Sheep Host Fecal Proteome.

We recently reported the first characterization of the sheep fecal microbiota, obtained by using a multi-meta-omic approach. Here, the mass spectra generated by single-run LC/high-resolution MS in the context of that study were reanalyzed using a host-specific database, in order to gain insights for the first time into the host fecal proteome of healthy Sarda sheep. On the whole, 5349 non-redundant tryptic peptide sequences were identified, belonging to 1046 different proteins. The "core" fecal proteome (co...

Guidelines for Developing Successful Short Advanced Courses in Systems Medicine and Systems Biology.

Remodeling of the HDL proteome with treatment response to abatacept or adalimumab in the AMPLE trial of patients with rheumatoid arthritis.

To evaluate changes in the high-density lipoprotein (HDL) proteome and HDL function in active rheumatoid arthritis (RA) patients initiating therapy with abatacept or adalimumab in the Abatacept Versus Adalimumab Comparison in Biologic-Naïve RA Subjects with Background Methotrexate (AMPLE) study.

Proteome Profiling of Sertoli Cells Using a GeLC-MS/MS Strategy.

Proteomics is a technology that allows to decipher the molecular networks involved in the regulation of biological processes such as spermatogenesis. Sertoli cells (SCs) are key players in the paracrine control of this process. Envisioning to increase the knowledge on the molecular networks harbored in SCs, we propose a methodology based on GeLC-MS/MS for the characterization of these cells' proteome. Proteins are separated by SDS-PAGE hyphenated to HPLC and identified by mass spectrometry. The integration ...

Proteome Imbalance of Mitochondrial Electron Transport Chain in Brown Adipocytes Leads to Metabolic Benefits.

Brown adipose tissue (BAT) thermogenesis is critical for thermoregulation and contributes to total energy expenditure. However, whether BAT has non-thermogenic functions is largely unknown. Here, we describe that BAT-specific liver kinase b1 knockout (Lkb1) mice exhibited impaired BAT mitochondrial respiration and thermogenesis but reduced adiposity and liver triglyceride accumulation under high-fat-diet feeding at room temperature. Importantly, these metabolic benefits were also present in Lkb1mice at ther...

Fasting and refeeding induces changes in the mouse hepatic lipid droplet proteome.

During fasting, the liver increases lipid storage as a mean to reserve and provide energy for vital cellular functions. After re-feeding, hepatocytes rapidly decrease the amount of triacylglycerol that is stored in lipid droplets (LDs), visible as the size of hepatic LDs significantly decreases after re-feeding. Little is known about the changes in the liver LD proteome that occur during the fasting/re-feeding transition. This study aimed to investigate the hepatic LD proteome in fasted and re-fed condition...

Understanding the Lipid Droplet Proteome and Protein Targeting.

Reporting in this issue of Developmental Cell, Bersuker et al. (2018) adapt APEX technology to lipid droplets for a more accurate view of the droplet proteome and Prévost et al. (2018) provide important insights into the basis of droplet protein targeting that altogether extend the understanding of this organelle.


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