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PubMed Journals Articles About "TanTec Biosystems, Inc." RSS

04:14 EST 13th December 2018 | BioPortfolio

TanTec Biosystems, Inc. PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest TanTec Biosystems, Inc. articles that have been published worldwide.

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Showing "TanTec Biosystems" PubMed Articles 1–25 of 42

Systematic evaluation of the early access applied biosystems precision ID Globalfiler mixture ID and Globalfiler NGS STR panels for the ion S5 system.


Hydrodynamics and mathematical modelling in a low HRT inverse fluidized-bed reactor for biological sulphate reduction.

Biological reduction of sulphate at low hydraulic retention time (HRT) is presented in this paper. A sulphidogenic inverse fluidized-bed bioreactor (IFBB) was operated successfully at a progressively decreasing HRT from 1 to 0.125 days for a total of 155 days. Synthetic wastewater containing sulphate at a concentration of 745 (± 17) mg/L was used. COD was supplied as lactate in variable concentrations at COD/SO ratios of 1.2-2.4. The pH of the feed ranged between 5.2 and 6.2. The highest measured remo...

Acetone, butanol, and ethanol production from the green seaweed Enteromorpha intestinalis via the separate hydrolysis and fermentation.

Acetone, butanol, and ethanol (ABE) were produced following the separate hydrolysis and fermentation (SHF) method using polysaccharides from the green macroalgae Enteromorpha intestinalis as biomass. We focused on the optimization of enzymatic saccharification as pretreatments for the fermentation of E. intestinalis. Pretreatment was carried out with 10% (w/v) seaweed slurry and 270-mM HSO at 121 °C for 60 min. Monosaccharides (mainly glucose) were obtained from enzymatic hydrolysis with a 16-U/mL mixtur...


Evaluation of chromium removal efficiency at varying operating conditions of a novel bioelectrochemical system.

The assessment of chromium removal efficiency (CRE) was studied using experimental trials with variations in operating conditions for evaluating performance efficiency of the Bioelectrochemical system (BES). During the study, maximum CRE of 88.36 ± 8.16% was obtained at cathode pH 2 ± 0.1 from the varied cathode pH (pH 1, 2, 3, 4, 5, 6, 7). With varying reactor temperatures from 25 to 45 °C (25, 30, 35, 40, 45 °C), BES resulted in maximum CRE of 85.93 ± 9.62% at 40 °C. CRE increased fr...

Cloning, expression, and characterization of an alkaline protease, AprV, from Vibrio sp. DA1-1.

A novel alkaline protease (named AprV) gene from Vibrio sp. DA1-1 was cloned and expressed in Escherichia coli BL21 (DE3) pLysS. The sequence analysis showed the highest homology of 68% with the characterized protease from Alkalimonas collagenimarina AC40. The recombinant AprV was purified with the molecular weight of 28 kDa. The optimum temperature and pH were determined to be 55 °C and 10.0, respectively. The enzyme activity was slightly enhanced by Ca, Mg, Zn, Ba, and, however, was highly inhibited by...

Bacterial indoleacetic acid-induced synthesis of colloidal AgO nanocrystals and their biological activities.

The biosynthesis and biological activity of colloidal AgO nanocrystals have not been well studied, although they have potential applications in many fields. For the first time, we developed a reducing agent free, cost-effective technique for AgO biosynthesis using Xanthomonas sp. P5. The optimal conditions for AgO synthesis were 50 °C, pH 8, and 2.5 mM AgNO. Using these conditions the yield of AgO obtained at 10 h was about five times higher than that obtained at 12 h under unoptimized conditions. AgO ...

A dicyclic-type electrode-based biofilm reactor for simultaneous nitrate and Cr(VI) reduction.

A dicyclic-type electrode-based biofilm-electrode reactor (BER) was investigated for simultaneous removal of nitrate and Cr(VI). In the absence of Cr(VI), almost complete denitrification of 50 mg/L NO-N was achieved at a very low C/N ratio of 0.8 with the optimal current of 50 mA. Cr(VI) was removed by biological reduction and co-precipitation when Cr(VI) was taken as the only electron acceptor, and the removal efficiencies of Cr(VI) were 99.8%. In the coexistent system of nitrate and Cr(VI), nitrate remo...

The start of the Abiogenesis: Preservation of homochirality in proteins as a necessary and sufficient condition for the establishment of the metabolism.

Biosystems contain an almost infinite amount of vital important details, which together ensure their life. There are, however, some common structures and reactions in the systems: the homochirality of carbohydrates and proteins, the metabolism and the genetics. The Abiogenesis, or the origin of life, is probably not a result of a series of single events, but rather the result of a gradual process with increasing complexity of molecules and chemical reactions, and the prebiotic synthesis of molecules might n...

Startup strategy for nitrogen removal via nitrite in a BAF system.

A biological aerated filter (BAF) pilot plant consisting of two reactors (aerobic and anoxic one) was used to determine a strategy to remove nitrogen via nitrite. RNA/DNA analysis was performed to assess microbial activity and support chemical results. In less than 13 days the pilot plant was able to remove COD and suspended solids. Nitrogen removal via nitrite pathway could not be observed until day 130 when the empty bed contact time (EBCT) was set at 0.71 h. Nitrite was detected in the aerated BAF efflu...

A comparative study of eubacterial communities by PCR-DGGE fingerprints in anoxic and aerobic biotrickling filters used for biogas desulfurization.

Biological desulfurization has proven to be a process that is technically and economically feasible on using biotrickling filters that can be performed under aerobic and anoxic conditions. However, microbial communities are different mainly due to the use of different final electron acceptors. The analysis of microbial communities in these systems has not been addressed with regard to the anoxic process. The aim of the work reported here was to analyse the eubacterial community in the two types of bioreacto...

A novel osmotic pressure strategy to improve erythritol production by Yarrowia lipolytica from glycerol.

Polyethylene glycol (PEG) is polymer that was used to replace NaCl (reference media) as an osmotic stress agent for the synthesis of erythritol by the osmophilic yeast Yarrowia lipolytica. Two strains, the wild-type strain IMUFRJ 50682 and the lab strain W29, were grown in the presence of PEG of different molecular weights. For strain IMUFRJ 50682, the erythritol titer was increased by 40% in the presence of PEG2000 as compared to the reference media (with NaCl). A similar increase was also observed for str...

Bio-activity of aminosulfonyl ureas in the light of nucleic acid bases and DNA base pair interaction.

The quantum chemical descriptors based on density functional theory (DFT) are applied to predict the biological activity (log IC) of one class of acyl-CoA: cholesterol O-acyltransferase (ACAT) inhibitors, viz. aminosulfonyl ureas. ACAT are very effective agents for reduction of triglyceride and cholesterol levels in human body. Successful two parameter quantitative structure-activity relationship (QSAR) models are developed with a combination of relevant global and local DFT based descriptors for prediction...

Advances in the slow freezing cryopreservation of microencapsulated cells.

Over the past few decades, the use of cell microencapsulation technology has been promoted for a wide range of applications as sustained drug delivery systems or as cells containing biosystems for regenerative medicine. However, difficulty in their preservation and storage has limited their availability to healthcare centers. Because the preservation in cryogenic temperatures poses many biological and biophysical challenges and that the technology has not been well understood, the slow cooling cryopreservat...

Enzymatic synthesis of cefazolin using immobilized recombinant cephalosporin-acid synthetase as the biocatalyst.

A method for the synthesis of β-lactam antibiotic cefazolin (CEZ) by enzymatic acylation of 7-amino-3-(5-methyl-l,3,4-thiadiazol-2-yl)thiomethyl-3-cephem-4-carboxylic acid (TDA) using immobilized cephalosporin-acid synthetase (IECASA) from recombinant E. coli strain VKPM B-12316 has been developed. A stepwise pH gradient designed on the basis of investigations on the solubility of components was applied for synthesis. This helped in avoiding the precipitation of TDA in the reaction when its initial concent...

Outdoor microalgae cultivation in airlift photobioreactor at high irradiance and temperature conditions: effect of batch and fed-batch strategies, photoinhibition, and temperature stress.

The microalgae Scenedesmus abundans cultivated in five identical airlift photobioreactors (PBRs) in batch and fed-batch modes at the outdoor tropical condition. The microalgae strain S. abundans was found to tolerate high temperature (35-45 °C) and high light intensity (770-1690 µmol m s). The highest biomass productivities were 152.5-162.5 mg L day for fed-batch strategy. The biomass productivity was drastically reduced due to photoinhibition effect at a culture temperature of > 45 °C. The lip...

Emerging techniques for cell disruption and extraction of valuable bio-molecules of microalgae Nannochloropsis sp.

Microalgae of Nannochloropsis sp. present valuable source of bio-molecules (pigments, lipids, proteins) that have nutritional potential for the prevention and treatment of human diseases. Moreover, some species of Nannochloropsis are the promising sources of biofuels and excellent candidates for the replacement of classical biofuel crops. This review describes and compares the efficiency of different conventional and novel techniques that can be used for cell disruption and recovery of bio-molecules from N...

Simultaneous acetone-butanol-ethanol fermentation, gas stripping, and full-cell-catalyzed esterification for effective production of butyl oleate.

In this study, aiming to improve the economic feasibility of acetone-butanol-ethanol (ABE) fermentation process, generate valuable products and extend the product chain, esterification catalyzed by Candida sp. 99-125 cells was hybrid with the ABE fermentation-gas-stripping integration system. The gas-stripping condensate that contained concentrated ABE products was directly used for esterification without the participation of toxic organic solvents. Full-cell catalysis temperature and the cell dosage rate o...

Polymersomes as nanoreactors for preparative biocatalytic applications: current challenges and future perspectives.

Polymersomes are hollow, spherical vesicles that are surrounded by a polymer membrane. The applied polymer must be amphiphilic to promote self-assembly in aqueous solution. At the same time, the polymer composition is highly versatile, which leads to diverse properties in terms of chemical and mechanical stability, membrane permeability and the ability to functionalize the membrane. By encapsulating chemical or biological substances within the polymersomes, drug delivery systems, cell mimetics or catalytic ...

Potential of in situ SSF laccase produced from Ganoderma lucidum RCK 2011 in biobleaching of paper pulp.

Production of laccase from Ganoderma lucidum RCK 2011 under solid-state fermentation (SSF) conditions was optimized using response surface methodology, resulting in an approximate eightfold increase compared to that in the unoptimized media. Further, the enzyme produced under SSF as whole fermented substrate (in situ SSF laccase) was found to be more stable than the in vitro enzyme (harvested by downstreaming processing of fermented wheat bran). Interestingly, the biobleaching potentials of both in situ and...

Microbial Ligninolysis: Toward a Bottom-Up Approach for Lignin Upgrading.

Lignin, an underutilized source of biomass, is a challenging target for decomposition by both biological and nonbiological approaches. As lignin accounts for 15-40% of the dry weight of lignocellulose, its conversion to valued chemicals is considered promising. Although depolymerization of the complicated lignin structures is still not fully understood, biochemical reactions for various lignin-derived compounds, especially dimeric and monomeric aromatics, have been characterized in some fungi and bacteria k...

Investigation of copper inhibition of nitrifying moving bed biofilm (MBBR) reactors during long term operations.

Copper, a prevalent heavy metal in industrial mining wastewaters, has been shown to inhibit nitrification in wastewater treatment systems. Biofilm treatment systems have an inherent potential to reduce inhibition. This study investigated the effects of copper concentration on nitrifying biofilms in moving bed biofilm reactor (MBBR) systems across long term operation using influent ammonia concentrations representative of gold mining wastewater. Conventional isotherm models did not adequately model the attac...

Ethanol production from xylose is highly increased by the Kluyveromyces marxianus mutant 17694-DH1.

Directed evolutionary approach and random mutagenesis were performed on thermotolerant yeast Kluyveromyces marxianus KCTC17694 for isolating a yeast strain producing ethanol from xylose efficiently. The isolated mutant strain, K. marxianus 17694-DH1, showed 290% and 131% improvement in ethanol concentration and ethanol production yield from xylose, respectively, as compared with the parental strain. Sequencing of the KmXYL1 gene of K. marxianus 17694-DH1 revealed substitutions of arginine and tryptophan wit...

Enhanced production of succinic acid from methanol-organosolv pretreated Strophanthus preussii by recombinant Escherichia coli.

A biorefinery process for high yield production of succinic acid from biomass sugars was investigated using recombinant Escherichia coli. The major problem been addressed is utilization of waste biomass for the production of succinic acid using metabolic engineering strategy. Here, methanol extract of Strophanthus preussii was used for fermentation. The process parameters were optimized. Glucose (9 g/L), galactose (4 g/L), xylose (6 g/L) and arabinose (0.5 g/L) were the major sugars present in the metha...

A Liquid-Metal-Based Magnetoactive Slurry for Stimuli-Responsive Mechanically Adaptive Electrodes.

Electrical communication between a biological system and outside equipment allows one to monitor and influence the state of the tissue and nervous networks. As the bridge, bioelectrodes should possess both electrical conductivity and adaptive mechanical properties matching the target soft biosystem, but this is still a big challenge. A family of liquid-metal-based magnetoactive slurries (LMMSs) formed by dispersing magnetic iron particles in a Ga-based liquid metal (LM) matrix is reported here. The mechanic...

Encapsulating microorganisms inside electrospun microfibers as a living material enables room-temperature storage of microorganisms.

Room-temperature storage and transportation of microorganisms maximize the power of microorganisms in healthcare, energy and environment. Recently, paper-based biotechnologies have been developed to enable room-temperature storage of a variety of non-living biosystems such as diagnostic devices and cell-free systems. Herein, room-temperature storage of living microorganisms is realized by an electrospun nonwoven paper containing convex region, which is composed of coiled microfibers with dense distribution ...


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