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PubMed Journals Articles About "Thermo Fisher Scientific Nets FOPO" RSS

03:13 EDT 17th October 2018 | BioPortfolio

Thermo Fisher Scientific Nets FOPO PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest Thermo Fisher Scientific Nets FOPO articles that have been published worldwide.

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Showing "Thermo Fisher Scientific nets FOPO" PubMed Articles 1–25 of 3,900+

In lens BSE detector with energy filtering.

We present a new type of an in-lens detector designed for Thermo Fisher Scientific (FEI) electron microscopes with the Elstar column. A key feature of it is high-pass energy filtering to enable the detection of low-loss backscattered electrons with their energy close to the primary beam energy. We show an application of the detector in imaging of a biological sample where the signal from these electrons leads to a significant improvement in resolution.


Evaluation of the precision ID mtDNA whole genome panel on two massively parallel sequencing systems.

Sequencing whole mitochondrial genomes by capillary electrophoresis is a costly and time/labor-intensive endeavor. Many of the previous Sanger sequencing-based approaches generated amplicons that were several kilobases in length; lengths that are likely not amenable for most forensic applications. However, with the advent of massively parallel sequencing (MPS) short-amplicon multiplexes covering the entire mitochondrial genome can be sequenced relatively easily and rapidly. Recently, the Precision ID mtDNA ...

Neutrophil Extracellular Traps in the Second Decade.

Nearly 15 years after the first description of neutrophil extracellular traps (NETs), our knowledge concerning this structure has expanded considerably. Initially, NETs were considered solely an elaborate function of the innate immune system to combat invading microorganisms. Successively it became clear that NETs have farther-reaching capabilities. They are involved in a series of pathophysiological mechanisms ranging from inflammation to thrombosis where they fulfill essential functions when produced at t...


Magnetic nano-nets for capture of microbes in solution based on physical contact.

Self-assembly of Au nanoparticles with Fe ions is used to develop magnetic nano-nets similar to fishing nets for capture and removal of microbes in aqueous medium. The nano-nets have a high aspect ratio, span microns in length with openings of 80-300 nm. This allows them to sample the liquid medium even at low volume fraction and also entrap the microbes in the solution. The nets and the trapped microbes can be effectively pulled from the solution by using an off the shelf magnet. Since the capture is bas...

Generalization and Expressivity for Deep Nets.

Along with the rapid development of deep learning in practice, theoretical explanations for its success become urgent. Generalization and expressivity are two widely used measurements to quantify theoretical behaviors of deep nets. The expressivity focuses on finding functions expressible by deep nets but cannot be approximated by shallow nets with similar number of neurons. It usually implies the large capacity. The generalization aims at deriving fast learning rate for deep nets. It usually requires small...

Relative antibacterial functions of complement and NETs: NETs trap and complement effectively kills bacteria.

Neutrophil extracellular traps (NETs) are web-like DNA structures released by activated neutrophils. These structures are decorated with antimicrobial proteins, and considered to trap and kill bacteria extracellularly. However, the exact functions of NETs remain elusive, and contradictory observations have been made with NETs functioning as an antimicrobial or a pathogentrapping mechanism. There is a disconnect in the interpretation of the involvement of other major immune mechanisms, such as the complement...

Nutrition and neuroendocrine tumors: An update of the literature.

Neuroendocrine tumors (NETs) are a heterogeneous group of neoplasms with worldwide increasing incidence, high prevalence and survival. Both the tumor itself and the systemic therapy may have an impact on patients' nutrition. Malnutrition negatively impacts on outcome in NETs patients. Moreover, it has been demonstrated that body mass index was a risk factor for NET development and that metabolic syndrome was associated with worse prognosis in these patients. Of note, food could also interact with the metabo...

Near-Infrared-Fluorescent Erythrocyte-Mimicking Particles: Physical and Optical Characteristics.

Exogenous fluorescent materials activated by near-infrared (NIR) light can offer deep optical imaging with sub-cellular resolution, and enhanced image contrast. We have engineered NIR particles by doping hemoglobin-depleted erythrocyte ghosts (EGs) with indocyanine green (ICG). We refer to these optical particles as NIR erythrocyte-mimicking transducers (NETs). A particular feature of NETs is that their diameters can be tuned from micrometer to nanometer scale, thereby, providing a capability for broad NIR ...

Hamiltonian Transformation to Compute Thermo-osmotic Forces.

If a thermal gradient is applied along a fluid-solid interface, the fluid experiences a thermo-osmotic force. In the steady state, this force is balanced by the gradient of the shear stress. Surprisingly, there appears to be no unique microscopic expression that can be used for computing the magnitude of the thermo-osmotic force. Here we report how, by treating the mass M of the fluid particles as a tensor in the Hamiltonian, we can eliminate the balancing shear force in a nonequilibrium simulation and ther...

Finding the Optimal Nets for Self-Folding Kirigami.

Three-dimensional shells can be synthesized from the spontaneous self-folding of two-dimensional templates of interconnected panels, called nets. However, some nets are more likely to self-fold into the desired shell under random movements. The optimal nets are the ones that maximize the number of vertex connections, i.e., vertices that have only two of its faces cut away from each other in the net. Previous methods for finding such nets are based on random search, and thus, they do not guarantee the optima...

Microfluidic fabrication of biocompatible poly(N-vinylcaprolactam)-based microcarriers for modulated thermo-responsive drug release.

Various thermo-responsive polymers have been developed for controlled drug delivery upon the local application of external heat. The development of thermo-responsive polymers of high biocompatibility and tunable thermo-sensitivity is crucial for safe and efficient therapeutic application. In this study, thermo-responsive drug carriers featuring tunable thermo-sensitivities were synthesized using biocompatible poly(N-vinyl caprolactam) (PVCL) and stop-flow lithography. The PVCL-based particles showed selecti...

Evaluation of the precision ID whole MtDNA genome panel for forensic analyses.

Mitochondrial DNA (mtDNA) amplification and Massively Parallel Sequencing (MPS) using an early access version of the Precision ID Whole MtDNA Genome Panel (Thermo Fisher Scientific) and the Ion Personal Genome Machine (PGM) were evaluated using 15 forensically relevant samples. Samples were selected to represent typical forensic specimens for mtDNA analysis including hairs, hair shafts, swabs and ancient solid tissue samples (bones and teeth) that were stored in the freezer for up to several years after hav...

Management of Small Bowel Neuroendocrine Tumors.

Small bowel neuroendocrine tumors (NETs) are increasing in incidence and are now the most common primary malignancies of the small intestine. Despite this increase, the vague presentation and slow growth of these tumors lead to long delays in diagnosis, and many patients present with metastases. Patients with metastatic small bowel NETs have a favorable disease prognosis, particularly when contrasted with other GI malignancies, and benefit from aggressive, multimodal therapy. During the past decade, the opt...

IRF-1 Intervention in the Classical ROS-Dependent Release of NETs during LPS-Induced Acute Lung Injury in Mice.

Previously, we demonstrated that neutrophil extracellular traps (NETs) play an essential role in lipopolysaccharide (LPS)-induced acute lung injury. However, the underlying mechanism is unclear. In this study, we showed that knockout of interferon regulatory factor 1 (IRF-1) in mice strongly attenuated the generation of NETs and reactive oxygen species (ROS) production in neutrophils from bronchoalveolar lavage fluid and alleviated LPS-induced lung injury and systemic inflammation. Our in vitro experiments ...

Grade 3 Neuroendocrine Tumor (G3 NET) in a Background of Multiple Serotonin Cell Neoplasms of the Ileum Associated with Carcinoid Syndrome and Aggressive Behavior.

Grade 3 well-differentiated neuroendocrine tumors (G3 NETs) have been characterized in the pancreas and stomach and distinguished from low-to-intermediate grade (G1-G2) NETs, as well as from highly malignant, poorly differentiated neuroendocrine carcinomas (NECs). Up to now, no G3 NET has been thoroughly described in the distal small intestine. We herein report a case of a 61-year-old man presenting with carcinoid syndrome. The surgical specimen showed, in a background of small G1 ileal NETs, a larger, grad...

Nickel (II) nitrate hexahydrate triggered canine neutrophil extracellular traps release in vitro.

Nickel (II) nitrate hexahydrate (Ni) is a common heavy metal material in battery manufacturing, electroplating alloy parts and ceramic staining, therefore we frequently contact with Ni-related products in daily life. In this study, we aimed to investigate the effects of Ni on neutrophils extracellular traps (NETs) release by canine polymorphonuclear neutrophils (PMNs). The structure of Ni-induced NETs was observed by fluorescence confocal microscopy. Ni-triggered NETs release was quantified by Pico Green an...

Rectal neuroendocrine tumors (NETs) treated by ESD with myectomy - video vignette.

Rectal neuroendocrine tumors (NETs) smaller than 10 mm in diameter are generally considered to be less malignant and suitable for endoscopic treatment【1,2】. However, since NETs grow like submucosal tumors (SMTs), conventional endoscopic mucosal resection (EMR) may result in a positive vertical margin. Although the usefulness of endoscopic submucosal dissection (ESD) has recently been reported, no standard treatment has been established. This article is protected by copyright. All rights reserved.

Response mechanisms induced by exposure to high temperature in anthers from thermo-tolerant and thermo-sensitive tomato plants: A proteomic perspective.

Constant global warming is one of the most detrimental environmental factors for agriculture causing significant losses in productivity as heat stress (HS) conditions damage plant growth and reproduction. In flowering plants such as tomato, HS has drastic repercussions on development and functionality of male reproductive organs and pollen. Response mechanisms to HS in tomato anthers and pollen have been widely investigated by transcriptomics; on the contrary, exhaustive proteomic evidences are still lackin...

Erythrocyte-Derived Theranostic Nanoplatforms for Near Infrared Fluorescence Imaging and Photodestruction of Tumors.

Nanoparticles activated by near infrared (NIR) excitation provide a capability for optical imaging and photo-destruction of tumors. We have engineered optical nano-constructs derived from erythrocytes, which are doped with the FDA-approved NIR dye, indocyanine green (ICG). We refer to these constructs as NIR erythrocyte-mimicking transducers (NETs). Herein, we investigate the photo-theranostic capabilities of NETs for fluorescence imaging and photo-destruction of SKBR3 breast cancer cells, and subcutaneous ...

Peptidylarginine deiminase 4: a nuclear button triggering neutrophil extracellular traps in inflammatory diseases and aging.

Peptidylarginine deiminase 4 (PAD4) is a nuclear citrullinating enzyme that is critically involved in the release of decondensed chromatin from neutrophils as neutrophil extracellular traps (NETs). NETs, together with fibrin, are implicated in host defense against pathogens; however, the formation of NETs (NETosis) has injurious effects that may outweigh their protective role. For example, PAD4 activity produces citrullinated neoantigens that promote autoimmune diseases, such as rheumatoid arthritis, to whi...

177Lu-DOTATATE therapy in patients with neuroendocrine tumours including high-grade (WHO G3) neuroendocrine tumours: response to treatment and long-term survival update.

Upon diagnosis, distant metastases are encountered in 21-50% of neuroendocrine tumours (NETs). However, few systemic treatment options are available for the well-differentiated NETs in the metastatic stage. Lu-DOTATATE is one of the most effective treatments in this limited patient group. We retrospectively investigated its efficacy and effect on the survival in patients with both well-differentiated and grade III NETs who had high uptake in pretherapeutic Ga-DOTATATE PET/computed tomography scans.

Neutrophil extracellular trap production contributes to pathogenesis in SIV-infected nonhuman primates.

Neutrophil extracellular traps (NETs) are involved in the pathogenesis of many infectious diseases, yet their dynamics and impact on HIV/SIV infection were not yet assessed. We hypothesized that SIV infection and the related microbial translocation trigger NET activation and release (NETosis), and investigated the interactions between NETs and immune cell populations and platelets. We compared and contrasted the levels of NETs between SIV-uninfected, SIV-infected, and SIV-infected antiretroviral-treated non...

Electrochemistry Coupled Mesoscale Complexations in Electrodes Lead to Thermo-Electrochemical Extremes.

Thermo-electrochemical extremes continue to remain a challenge for lithium-ion batteries. Contrary to the conventional approach, we propose herein that the electrochemistry coupled and microstructure mediated cross-talk between the positive and negative electrodes ultimately dictates the off-equilibrium coupled processes, such as heat generation and the propensity for lithium plating. The active particle morphological differences between the electrode couple foster thermo-electrochemical hysteresis, where d...

Colorectal Neuroendocrine Neoplasms - areas of unmet need.

The subject of colorectal neuroendocrine neoplasms (NENs), subdivided into well-differentiated NENs, termed neuroendocrine tumours (NETs; grade (G) 1 and 2), and poorly-differentiated NENs, termed neuroendocrine carcinomas (NECs; G3) according to the 2010 World Health Organisation (WHO) classification, has arguably not had as much attention or study as NENs occurring in other sites. Colorectal NETs and NECs are however easier to study than many others since they are usually not difficult to remove and are i...

Entering the third decade of experience with octreotide LAR in neuroendocrine tumors: A review of current knowledge.

Gastroenteropancreatic neuroendocrine tumors (NETs) are a relatively rare group of heterogeneous neoplasms. The most significant advance in therapy of NETs has been the advent of the somatostatin analog octreotide, which represents a cornerstone in their management and dramatically changed the therapeutic landscape. Octreotide long-acting release (LAR) was developed to overcome some of the limitations of octreotide. Several clinical studies, including PROMID and RADIANT-2, have validated the clinical benefi...


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