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PubMed Journals Articles About "Microfluidic Device Help Doctors Separate Tumour Cells From" RSS

19:47 EDT 19th June 2019 | BioPortfolio

Microfluidic Device Help Doctors Separate Tumour Cells From PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest Microfluidic Device Help Doctors Separate Tumour Cells From articles that have been published worldwide.

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Showing "microfluidic device help doctors separate tumour cells from" PubMed Articles 1–25 of 26,000+

A Microfluidic Micropipette Aspiration Device to Study Single-Cell Mechanics Inspired by the Principle of Wheatstone Bridge.

The biomechanical properties of single cells show great potential for early disease diagnosis and effective treatments. In this study, a microfluidic device was developed for quantifying the mechanical properties of a single cell. Micropipette aspiration was integrated into a microfluidic device that mimics a classical Wheatstone bridge circuit. This technique allows us not only to effectively alter the flow direction for single-cell trapping, but also to precisely control the pressure exerted on the aspira...


Creating a capture zone in microfluidic flow greatly enhances the throughput and efficiency of cancer detection.

Efficient capture of rare circulating tumor cells (CTCs) from blood samples is valuable for early cancer detection to improve the management of cancer. In this work, we developed a highly efficient microfluidics-based method for detecting CTCs in human blood. This is achieved by creating separate capture and flow zones in the microfluidic device (ZonesChip) and using patterned dielectrophoretic force to direct cells from the flow zone into the capture zone. This separation of the capture and flow zones mini...

Thermopneumatic suction integrated microfluidic blood analysis system.

Blood tests provide crucial diagnostic information regarding several diseases. A key factor that affects the precision and accuracy of blood tests is the interference of red blood cells; however, the conventional methods of blood separation are often complicated and time consuming. In this study, we devised a simple but high-efficiency blood separation system on a self-strained microfluidic device that separates 99.7 ± 0.3% of the plasma in only 6 min. Parameters, such as flow rate, design of the filter tr...


A Multiwell Microfluidic Device for Analyzing and Screening Nonhormonal Contraceptive Agents.

Birth control and family planning play pivotal roles in the economic growth and reduction of maternal, infant, and child mortality. Current contraceptives, such as hormonal agents and intrauterine devices, target only a small subset of reproductive processes and can have serious side effects on the health of women. To develop novel contraceptive agents, a scalable microfluidic device is established for analyzing and screening the effects of potential contraceptive agents on the maturation of the cumulus-ooc...

Fabrication of composite microfluidic devices for local control of oxygen tension in cell cultures.

Oxygen tension is a central component of the cellular microenvironment and can serve as a trigger for changes in cell phenotype and function. There is a strong need to precisely control and modulate oxygen tension in cell culture systems in order to more accurately model the physiology and pathophysiology observed in vivo. The objective of this paper was to develop a simple, yet effective strategy for local control of oxygen tension in microfluidic cell cultures. Our strategy relied on fabrication of microf...

The use of a microfluidic device to encapsulate a poorly water-soluble drug CoQ in lipid nanoparticles and an attempt to regulate intracellular trafficking to reach mitochondria.

A number of drugs that are currently on the market, as well as new candidates for drugs are poorly water soluble. Because of this, a need exists to develop drug formulations that will permit the expanded use of such drugs. The use of liposomes and lipid nanoparticles (LNP) for drug delivery has attracted attention as a technique for solubilizing molecules that are poorly water-soluble, but this technique faces serious scale-up risks. In this study, we report on attempts to encapsulate Coenzyme Q (CoQ) as a ...

Stromal-induced mitochondrial re-education: impact on epithelial-to-mesenchymal transition and cancer aggressiveness.

Metabolic reprogramming as well as the flexible utilisation of fuel sources by tumour cells has been considered not only intrinsic to malignant cells but also sustained by resident and/or recruited stromal cells. The complexity of tumour-stroma cross-talk is experienced by neoplastic cells through profound changes in the own metabolic machinery. In such context, mitochondria are dynamic organelles that receive, orchestrate and exchange a multiplicity of stromal cues within the tumour cells to finely regulat...

Natural killer cells involved in tumour immune escape of hepatocellular carcinomar.

Natural killer cells are the first line of host immune surveillance and play major roles in the defence against infection and tumours. Hepatic NK cells exhibit unique phenotypic and functional characteristics compared to circulating and spleen NK cells, such as higher levels of cytolytic activity and cytotoxicity mediators against tumour cells. However, the activities of NK cells may be reversed during tumour progression. Recent studies demonstrated that hepatic NK cells were exhausted in hepatocellular car...

Dynamic fluorescent imaging analysis of mitochondrial redox in single cells with a microfluidic device.

The redox balance in cellular mitochondria is closely related to the physiological and pathological processes of the body. When exposed to external stimuli, the redox state in cells changes dynamically, and presents cell heterogeneity, which creates a need for techniques that can make dynamic and reversible visual analysis of redox in mitochondria at single-cell level. Here we describe a method for single-cell redox analysis based on a microfluidic device combing with a reversible fluorescent probe (Cy-O-eb...

A highly integrated real-time digital PCR device for accurate DNA quantitative analysis.

Misclassification of positive partitions in microfluidic digital polymerase chain reaction (dPCR) can cause the false positives and false negatives, which significantly alter the resulting estimate of target DNA molecules. To address this issue, establishing real-time fluorescence interrogation of each partition in microfluidic arrays is an effective way in which false positive and false negative partitions can be eliminated. However, currently available devices for real-time fluorescence interrogation are ...

Tumour Parcellation and Quantification (TuPaQ): a tool for refining biomarker analysis through rapid and automated segmentation of tumour epithelium.

Immunohistochemistry (IHC) is an essential component of biomarker research in cancer. Automated biomarker quantification is hampered by the failure of computational algorithms to discriminate "negative" tumour cells from "negative" stromal cells. We sought to develop an algorithm for segmentation of tumour epithelium in colorectal cancer (CRC) irrespective of the biomarker expression in the cells.

Microfluidic Immiscible Phase Filtration System for the Isolation of Small Numbers of Cells from Whole Blood.

Isolation of circulating tumor cells (CTCs) has generated clinical and academic interest due to the important role that CTCs play in cancer metastasis and diagnosis. Here, we present a PDMS and glass prototype of a microfluidic device for the immunomagnetic, immiscible phase filtration based capture, and isolation of MCF-7 breast cancer cells, from various sample matrices including PBS-based buffer, blood plasma, and unprocessed whole blood. Following optimization of surface energy of an oil-water interface...

PDMS-free microfluidic cell culture with integrated gas supply through a porous membrane of anodized aluminum oxide.

Microfluidic cell cultures are often used in academic research but only rarely in pharmaceutical research because of unsuitable designs, inappropriate choice of materials or incompatibility with standard equipment. In particular, microfluidic cell cultures to control the gaseous microenvironment rely on PDMS despite its disadvantages. We present a novel concept for such a cell culture device that addresses these issues and is made out of hard materials instead of PDMS. Our device contains two microfluidic c...

Cooperation among cancer cells: applying game theory to cancer.

Cell cooperation promotes many of the hallmarks of cancer via the secretion of diffusible factors that can affect cancer cells or stromal cells in the tumour microenvironment. This cooperation cannot be explained simply as the collective action of cells for the benefit of the tumour because non-cooperative subclones can constantly invade and free-ride on the diffusible factors produced by the cooperative cells. A full understanding of cooperation among the cells of a tumour requires methods and concepts fr...

Microfluidic device for the analysis of MDR cancerous cell-derived exosomes' response to nanotherapy.

Exosomes are membrane-enclosed extracellular vesicles which have been indicated as important biomarkers of cancerous cell functionality, such as multiple drug resistance (MDR). Nanoparticles based chemotherapy is a promising strategy to overcome MDR by interfering the production and composition of exosomes. Therefore, tumor-derived exosomes post-treatment by nanotherapy are implied to play critical roles of biomarkers on cancer MDR analysis. However, the efficient isolation of such exosomes from extracellul...

Impedimetric array in polymer microfluidic cartridge for low cost point-of-care diagnostics.

Deep Vein Thrombosis and pulmonary embolism (DVT/PE) is one of the most common causes of unexpected death for hospital in-patients. D-dimer is used as a biomarker within blood for the diagnosis of DVT/PE. We report a low-cost microfluidic device with a conveniently biofunctionalised interdigitated electrode (IDE) array and a portable impedimetric reader as a point-of-care (POC) device for the detection of D-dimer to aid diagnosis of DVT/PE. The IDE array elements, fabricated on a polyethylenenaphtalate (PEN...

A Polyoxometalate-Based Microfluidic Device for Liquid Phase Oxidation of Glycerol.

Peroxidation of glycerol has been performed in microfluidic reactor based on polyoxometalates (POMs), which has been fabricated on a capillary using a layer-by-layer strategy. Lactic acid (LA) is produced selectively in high yield with a TOF as high as 20,000 h-1 compared to a TOF of 200 h-1 in batch mode. This POM microfluidic reactor is readily prepared, scalable, highly stable, reusable, and also potentially applicable to selective oxidation of other bio-wastes.

Targeting nucleolin to obstruct vasculature feeding with an intelligent DNA nanorobot.

A prototype of DNA nanorobot with the ability to transport molecular payloads was designed to target cancer cells in tissue culture. Moreover, a further step was taken to succeed in the first in vivo application of the DNA nanorobot for cancer therapy. The robot was constructed using aptamer and DNA origami to fold a 90-nm tubular device to carry the blood coagulation protease thrombin inside, shielded from circulating platelets and plasma fibrinogen. The recognition and binding of the aptamer to its tumou...

The clinical role of the TME in solid cancer.

The highly complex and heterogenous ecosystem of a tumour not only contains malignant cells, but also interacting cells from the host such as endothelial cells, stromal fibroblasts, and a variety of immune cells that control tumour growth and invasion. It is well established that anti-tumour immunity is a critical hurdle that must be overcome for tumours to initiate, grow and spread and that anti-tumour immunity can be modulated using current immunotherapies to achieve meaningful anti-tumour clinical respon...

Putative cancer stem cells are present in surgical margins of oral squamous cell carcinoma.

Recent evidence suggests that small subpopulations of stem-like cells are accountable for tumour initiation, progression and metastasis. Until now, studies were focused exclusively on the characterization of these cell populations within the tumour itself, while tumour margins were neglected, although it is known that the histological and molecular status of tumour margins may play a significant role in the course of the disease. Therefore, the aims of the study were to isolate cells from oral squamous cell...

Pericytes in Metastasis.

Pericytes have long been known to contribute indirectly to tumour growth by regulating angiogenesis. Thus, remodelling tumour blood vessels to maintain blood supply is critical for continued tumour growth. A role for pericytes in restricting leakage of tumour cells through blood vessels has also become evident given that adequate pericyte coverage of these blood vessels is critical for maintaining vascular permeability. Interestingly, the relocation of pericytes from blood vessels to the tumour microenviron...

Microfluidic device to attain high spatial and temporal control of oxygen.

Microfluidic devices have been successfully used to recreate in vitro biological microenvironments, including disease states. However, one constant issue for replicating microenvironments is that atmospheric oxygen concentration (21% O2) does not mimic physiological values (often around 5% O2). We have created a microfluidic device that can control both the spatial and temporal variations in oxygen tensions that are characteristic of in vivo biology. Additionally, since the microcirculation is responsive to...

Microfluidic Devices Containing ZnO Nanorods with Tunable Surface Chemistry and Wetting Independent Water Mobility.

IInterest in PDMS microfluidic devices has grown dramatically in recent years, particularly in the context of improved performance lab-on-a-chip devices with decreasing channel size enabling more devices on ever smaller chips. As channels become smaller, the resistance to flow increases and the device structure must be able to withstand higher internal pressures. We report herein the fabrication of microstructured surfaces that promote water mobility independent of surface static wetting properties. The key...

Microfluidic synchronizer using a synthetic nanoparticle-capped bacterium.

Conventional techniques to synchronize bacterial cells often require manual manipulations and lengthy incubation lacking precise temporal control. An automated microfluidic device was recently developed to overcome these limitations. However, it exploits the stalk property of Caulobacter crescentus that undergoes asymmetric stalked and swarmer cell cycle stages and is therefore restricted to this species. To address this shortcoming, we have engineered Escherichia coli cells to adhere to microchannel walls ...

TUMOR BUDDING: PROGNOSTIC VALUE IN MUSCLE-INVASIVE BLADDER CANCER.

To assess if 'tumour budding' (TB) behaves as a poor prognostic factor in muscle-invasive bladder carcinoma (MIBC). TB is the presence of tumour cells isolated or in small groups of fewer than five cells located at the tumour invasion front.


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