Topics

PubMed Journals Articles About "Genetically Targeted Fluorescent Probes Reveal Dynamic Calcium Responses" RSS

13:57 EDT 24th August 2019 | BioPortfolio

Genetically Targeted Fluorescent Probes Reveal Dynamic Calcium Responses PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest Genetically Targeted Fluorescent Probes Reveal Dynamic Calcium Responses articles that have been published worldwide.

More Information about "Genetically Targeted Fluorescent Probes Reveal Dynamic Calcium Responses" on BioPortfolio

We have published hundreds of Genetically Targeted Fluorescent Probes Reveal Dynamic Calcium Responses news stories on BioPortfolio along with dozens of Genetically Targeted Fluorescent Probes Reveal Dynamic Calcium Responses Clinical Trials and PubMed Articles about Genetically Targeted Fluorescent Probes Reveal Dynamic Calcium Responses for you to read. In addition to the medical data, news and clinical trials, BioPortfolio also has a large collection of Genetically Targeted Fluorescent Probes Reveal Dynamic Calcium Responses Companies in our database. You can also find out about relevant Genetically Targeted Fluorescent Probes Reveal Dynamic Calcium Responses Drugs and Medications on this site too.

Showing "Genetically Targeted Fluorescent Probes Reveal Dynamic Calcium Responses" PubMed Articles 1–25 of 20,000+

Biology and Significance of Alpha-Fetoprotein in Hepatocellular Carcinoma.

Hepatocellular carcinoma (HCC) is one of the most common causes of cancer-related deaths globally due, in part, to the majority of patients being diagnosed with intermediate or advanced stage disease. Our increased understanding of the heterogeneous molecular pathogenesis of HCC has led to significant developments in novel targeted therapies. Despite these advances, there remains a high unmet need for new treatment options. HCC is a complex disease with multiple pathogenic mechanisms caused by a variety of ...


Cluster miRNAs and cancer: Diagnostic, prognostic and therapeutic opportunities.

MiRNAs are class of noncoding RNA important for gene expression regulation in many plants, animals and viruses. MiRNA clusters contain a set of two or more miRNA encoding genes, transcribed together as polycistronic miRNAs. Currently, there are approximately 159 miRNA clusters reported in the human genome consisting of miRNAs ranging from two or more miRNA genes. A large proportion of clustered miRNAs resides in and around the fragile sites or cancer associated genomic hotspots and plays an important role i...

Cannabinoid receptor modulation changes the accumbal neuronal responses to morphine in the reinstatement of morphine-induced conditioned place preference.

The nucleus accumbens (NAc) is a central component of the brain reward system. It has been known that most of the drugs of abuse such as opioids and cannabinoids affect the NAc. Although cannabinoids can modulate different stages of morphine encounter such as the reinstatement of morphine-induced conditioned place preference (CPP), there is no evidence for the NAc neurons' response to prove it. That is why the present study was designed. The procedure was as follows: The rats were entered to CPP by sc 5 mg/...


Tetrasubstituted cyclopentenone-based fluorescent chemosensors for the selective detection of Fe and Cu ions.

Fluorescent chemosensors based on 4-hydroxy cyclopentenones were synthesized by the base catalyzed reaction of 1,5-diphenyl-pentane-1,3,5-trione with benzil and thenil. The molecule obtained by the benzil reaction was found to be useful for the selective detection of Fe by fluorescence turn-off, while the molecule synthesized by the thenil reaction was useful for selective detection of Cu by fluorescent turn-on. Details of the synthesis, complexation mode, nature of binding, reversibility, and pH studies of...

Discrete π-Stacks from Self-assembled Perylenediimide Lookalikes.

The formation of well-defined finite-size aggregates represents an attractive goal in supramolecular chemistry. In particular, construction of discrete π-stacked dye assemblies remains a challenge. Herein, we report on the design and synthesis of a novel type of discrete π-stacked aggregates from two comparable perylenediimide (PDI) dyads ( PEP and PBP ). The criss-cross PEP - PBP dimers in solution and ( PBP - PEP )-( PEP - PBP ) tetramers in solid state are well elucidated using single-crystal X-ray dif...

Rapid On-Demand Extracellular Vesicle Augmentation with Versatile Oligonucleotide Tethers.

Exosomes show potential as ideal vehicles for drug delivery because of their natural role in transferring biological cargo between cells. However, current methods to engineer exosomes without negatively impacting their function remain challenging. Manipulating exosome-secreting cells is complex and time-consuming, while direct functionalization of exosome surface proteins suffers from low specificity and low efficiency. We demonstrate a rapid, versatile and scalable method with oligonucleotide tethers to en...

The Structural Basis by Which the N-terminal Polypeptide Segment of Rhizopus chinensis Lipase Regulates Its Substrate Binding Affinity.

An important group of industrial enzymes, Rhizopus lipases exhibit valuable hydrolytic features that underlie their biological functions. Particularly important is their N-terminal polypeptide segment (NTPS), which is required for secretion and proper folding, but is removed in the process of enzyme maturation. A second common feature of this class of lipases is the α-helical "lid", which regulates accessibility of substrate to the enzyme active site. Some Rhizopus lipases also exhibit "interfacial activat...

Activating Graphyne Nanosheet via sp-Hybridized Boron Modulation for Electrochemical Nitrogen Fixation.

Exploring new metal-free catalysts with high activity for nitrogen reduction reaction (NRR) is highly desirable but remains a big challenge. Graphyne (GY) is a typical two-dimensional carbon material with many excellent properties. However, the NRR has rarely been envisaged on a GY-based metal-free catalyst up to now. Density functional theory calculations reveal that although pristine GY is inactive for N reduction, boron modulation can endow it with efficient activity toward NRR. Natural bond orbitals ana...

A NIR probe tracks and treats lung tumor initiating cells by targeting HMOX2.

Tumor initiating cells (TIC) are resistant to conventional anti-cancer therapy and associated with metastasis and relapse in cancer. Although various TIC markers and their antibodies have been proposed, it is limited to use of antibodies for in vivo imaging or treatment of TIC. In this study, we discovered heme oxygenase 2 (HMOX2) as a novel biomarker for TIC and developed a selective small molecule probe TiNIR (Tumor Initiating cell probe with Near Infra-Red). TiNIR detects and enriches the functionally ac...

Reduced-Order Dispatch Model for Simulating Marginal Emissions Factors for the United States Power Sector.

This study develops a reduced-order power plant dispatch model and uses it to simulate marginal emissions factors (MEFs) for the 2014-2017 United States (U.S.) electric grid at the North American Electric Reliability Corporation (NERC) regional level. MEFs help quantify the health, environmental, and climate change impacts caused by changes in marginal net electricity consumption, which could result, for example, from new technologies or policies. This study develops the model, validates it against historic...

Structure and Function of the Acetylpolyamine Amidohydrolase from the Deep Earth Halophile Marinobacter subterrani.

Polyamines are small organic cations essential for cellular function in all kingdoms of life. Polyamine metabolism is regulated by enzyme-catalyzed acetylation-deacetylation cycles in similar fashion to the epigenetic regulation of histone function in eukaryotes. Bacterial polyamine deacetylases are particularly intriguing, since these enzymes share the fold and function of eukaryotic histone deacetylases. Recently, acetylpolyamine amidohydrolase from the deep earth halophile Marinobacter subterrani (msAPAH...

Sequence Programming with Dynamic Boronic Acid/Catechol Binary Codes.

The development of a synthetic code that enables a sequence pro-grammable feature like DNA represents a key aspect toward intelli-gent molecular systems. We developed herein the well-known dynamic covalent interaction between boronic acids (BA) and catechols (CA) into synthetic nucleobase analogs. Along a defined peptide backbone, BA or CA residues are arranged to enable se-quence recognition to their complementary strand. Dynamic strand displacement and errors were elucidated thermodynamically to show that...

Ligand-Induced G-Quadruplex Polymorphism: A DNA Nanodevice for Label-Free Aptasensor Platforms.

G-Quadruplexes (GQs) serve as popular recognition elements for DNA aptasensors and are incorporated into a DNA nanodevice capable of controlled conformational changes to activate a sensing mechanism. Herein we highlight the utili-ty of a GQ-GQ nanodevice fueled by GQ-specific ligands as a label-free aptasensor detection strategy. The concept was first illustrated utilizing the prototypical polymorphic human telomeric repeat sequence (H-Telo22, d[AG3(T2AG3)3]) that can undergo ligand-induced topology changes...

Chemical Synthesis of Atomically Tailored SUMO E2 Conjugating Enzymes for the Formation of Covalently Linked SUMO-E2-E3 Ligase Ternary Complexes.

E2 conjugating enzymes are the key catalytic actors in the transfer of ubiquitin, SUMO, and other ubiquitin-like modifiers to their substrate proteins. Their high rates of transfer and promiscuous binding complicate studies of their interactions and binding partners. In order to access specific, covalently-linked conjugates of the SUMO E2 conjugating enzyme Ubc9, we prepared synthetic variants bearing site-specific non-native modifications including: 1) replacement of Cys93 to 2,3-diaminopropionic acid to f...

Nonadiabatic Photochemistry Induced by Inaccessible Conical Intersections.

Conical intersections are ubiquitous in photochemical processes, where nonadiabatic transfer induces ultrafast nonradiative decay from an excited state. Although they eluded experimental detection until the 1990s, today three diagnostic attributes are generally associated with photochemical reactions through conical intersections: ultrafast electronic dynamics, negligible fluorescence, and coherent wavepacket transfer. Here, we use generalized quantum dynamics simulations to show that coherent nonadiabatic ...

Tracking Magnetic Rotating Objects by Bipolar Electrochemiluminescence.

There is a very rapid development of original systems that can be remotely controlled or addressed by playing with chemical and physical concepts. Here, we present the synergetic combination of external magnetic and electric fields to promote, in a double contactless mode, the rotational motion and the concomitant generation of light-emission at the level of a gold-coated iron wire. The latter can be moved by rotating magnetic fields. Simultaneously, an electric field induces its remote polarization, which ...

The Emotional and Behavioral Impact of Delivering Bad News to Virtual versus Real Standardized Patients: A Pilot Study.

Virtual standardized patients (SPs) are becoming increasingly common in medical education, though one limitation of SPs is the artificiality of computer-based simulators. Past research on the use of SPs has not clearly established whether learners have different emotional responses to real SPs (SPs) compared with SPs; however, understanding learners' emotional responses to SPs is important in providing realistic learning experiences and establishing the validity of this teaching and assessment tool. This st...

Neurocognitive profile of outpatients with unipolar depressive disorders.

: It is widely accepted that major depressive disorder (MDD) is associated with impairments in several cognitive domains in inpatients, but cognitive functioning has barely been systematically investigated in depressed outpatients. The aims of the present study were to examine cognitive functioning in depressed outpatients in comparison with healthy controls and to determine whether cognitive deficits vary as a function of different disorder characteristics and demographic variables. : We compared 103 outpa...

PAVLOVIAN CONDITIONING OF IMMUNOLOGICAL AND NEUROENDOCRINE FUNCTIONS.

The phenomenon of behaviorally conditioned immunological and neuroendocrine responses has been investigated for the past 100 years. The observation that peripheral immune functions can be modified by associative learning processes was first reported and investigated by Ivan Petrovic Pavlov and his co-workers. Their work later fell into oblivion, also because so little was known about the immune system's function and even less about the underlying mechanisms of how learning, a central nervous system activity...

The Influence of Native- Versus Foreign-Accented Speech on Spanish-English Bilingual Children's Spanish Receptive Vocabulary Performance: A Pilot Study.

Purpose There are many fewer speech-language pathologists (SLPs) who can administer Spanish language testing than there are children in the United States who need such testing. Although there are precautions against language testing by foreign speakers, results from testers using native- versus foreign-accented speech have not been compared using popular picture-pointing vocabulary tests of the sort used by SLPs. Therefore, we sought to determine if nonnative Spanish speech (i.e., foreign-accented speech) w...

Effect of Negative Middle Ear Pressure and Compensated Pressure on Wideband Absorbance and Otoacoustic Emissions in Children.

Objective This study investigated pressurized transient evoked otoacoustic emission (TEOAE) responses and wideband absorbance (WBA) in healthy ears and ears with negative middle ear pressure (NMEP). Method In this cross-sectional study, TEOAE amplitude, signal-to-noise ratio, and WBA were measured at ambient and tympanometric peak pressure (TPP) in 36 ears from 25 subjects with healthy ears (age range: 3.1-13.0 years) and 88 ears from 76 patients with NMEP (age range: 2.0-13.1 years), divided into 3 groups ...

Readiness to Perform Aerobic Activity in Adults With Obesity: A Thematic Analysis of Online Surveys.

Flexible nonlinear periodization (FNLP) was designed to optimize sport-specific resistance training by matching bout demand to pre-exercise mental and physical states ("readiness to train"). Before applying this participant-responsive framework to aerobic prescriptions for inactive adults, operational definitions of readiness are needed. : To identify themes underlying readiness to perform aerobic bouts considered to be low-demand bouts (LDB) and high-demand bouts (HDB) in adults with obesity. : Using Qualt...

DNA methyltransferase inhibition overcomes diphthamide pathway deficiencies underlying CD123-targeted treatment resistance.

The interleukin-3 receptor alpha subunit, CD123, is expressed on many hematologic malignancies including acute myeloid leukemia (AML) and blastic plasmacytoid dendritic cell neoplasm (BPDCN). Tagraxofusp (SL-401) is a CD123-targeted therapy consisting of interleukin-3 fused to a truncated diphtheria toxin payload. Factors influencing response to tagraxofusp other than CD123 expression are largely unknown. We interrogated tagraxofusp resistance in patients and experimental models and found that it was not as...

Human Papillomavirus-Attributable Cancers - United States, 2012-2016.

Human papillomavirus (HPV) causes nearly all cervical cancers and some cancers of the vagina, vulva, penis, anus, and oropharynx (1).* Most HPV infections are asymptomatic and clear spontaneously within 1 to 2 years; however, persistent infection with oncogenic HPV types can lead to development of precancer or cancer (2). In the United States, the 9-valent HPV vaccine (9vHPV) is available to protect against oncogenic HPV types 16, 18, 31, 33, 45, 52, and 58 as well as nononcogenic types 6 and 11 that cause ...

Twelve quick tips for designing sound dynamical models for bioprocesses.

Because of the inherent complexity of bioprocesses, mathematical models are more and more used for process design, control, optimization, etc. These models are generally based on a set of biochemical reactions. Model equations are then derived from mass balance, coupled with empirical kinetics. Biological models are nonlinear and represent processes, which by essence are dynamic and adaptive. The temptation to embed most of the biology is high, with the risk that calibration would not be significant anymore...


Quick Search