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PubMed Journals Articles About "Protein Dynamic Solutions" RSS

17:54 EDT 18th July 2019 | BioPortfolio

Protein Dynamic Solutions PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest Protein Dynamic Solutions articles that have been published worldwide.

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Showing "Protein Dynamic Solutions" PubMed Articles 1–25 of 20,000+

End-diastolic force pre-activates cardiomyocytes and determines contractile force; role of titin and calcium.

Titin functions as a molecular spring and cardiomyocytes are able, through splicing, to control the length of titin. We hypothesized that together with diastolic [Ca ] titin-based stretch pre-activates cardiomyocytes during diastole and is a major determinant of force production in the subsequent contraction. Through this mechanism titin would play an important role in active force development and length-dependent activation. Mutations in the splicing factor RNA binding motif protein 20 (RBM20) result in ex...


Sunlight selectively modifies Maillard reaction products in a wide array of chemical reactions.

We report the photochemical transformation of Maillard reaction products (MRPs) under simulated sunlight into mostly unexplored photoproducts. Non-enzymatic glycation of amino acids leads to a heterogeneous class of intermediates with extreme chemical diversity, which is of particular relevance in processed and stored food products as well as in diabetic and age-related protein damage. We here reacted three amino acids (lysine, arginine, and histidine) with ribose at 100 °C for ten hours. Exposing these mo...

Antibodies against the DNABII protein integration host factor (IHF) inhibit sinus implant biofilms.

Chronic rhinosinusitis is a common, costly condition often treated with endoscopic sinus surgery and intraoperative placement of intranasal sinus implant materials. Whereas these materials aid in postoperative healing, they also support bacterial biofilm formation and thus contribute to negative outcomes. This study examined pretreatment of sinus implant materials with antibody against an essential bacterial biofilm structural component, the DNABII family of DNA-binding proteins, as a strategy to prevent bi...


The draft genome of a wild barley genotype reveals its enrichment in genes related to biotic and abiotic stresses compared to cultivated barley.

Wild barley (Hordeum spontaneum) is the progenitor of cultivated barley (H. vulgare) and provides a rich source of genetic variations for barley improvement. Currently, the genome sequences of wild barley and its differences with cultivated barley remain unclear. In this study, we report a high-quality draft assembly of wild barley accession (AWCS276; henceforth named as WB1), which consists of 4.28 Gb genome and 36,395 high-confidence protein coding genes. BUSCO analysis revealed that the assembly included...

Direct visualization of single nuclear pore complex proteins using genetically-encoded probes for DNA-PAINT.

The Nuclear Pore Complex (NPC) is one of the largest and most complex protein assemblies in the cell and - among other functions - serves as the gatekeeper of nucleocytoplasmic transport. Unraveling its molecular architecture and functioning has been an active research topic for decades with recent cryogenic electron microscopy and super-resolution studies advancing our understanding of the NPC's complex architecture. However, the specific and direct visualization of single copies of NPC proteins is thus fa...

A double armed, hydrophilic, transition metal complex as paramagnetic NMR probe.

Synthetic metal complexes find use as paramagnetic probes for the study of proteins and protein complexes. Here, two transition metal NMR probes (TraNPs) are reported. TraNPs are attached via two arms to a protein to generate pseudocontact shift (PCS) using cobalt (II), or paramagnetic relaxation enhancement (PRE) with manganese (II). The PCS analysis of TraNPs attached to three different proteins shows that the size of the anisotropic component of the magnetic susceptibility depends on the probe surroundin...

Powering the Hydrogen Economy from Waste Heat: A Review of Heat to Hydrogen Concepts.

Ever increasing energy demand and environmental concerns requires new and clean energy supplies, many of which are intermittent and non-correlating with demand. In order to balance supply with demand a universal energy vector can be employed such that intermittent renewable energy can be stored and transported and then used when needed. Hydrogen is the perfect universal energy vector and one of the possible solutions that ensures environmental cleanness and maximum utilisation of renewable energy sources an...

Lipid peroxidation increases hydrogen peroxide permeability leading to cell death in cancer cell lines that lack mtDNA.

Lipid peroxidation is a cause of cell and tissue injury. An important product of plasma membrane lipid peroxidation is HNE. We have established mtDNA-depleted ρ cells, which are subjected to higher oxidative stress versus their parental cells, using cervical cancer and oral squamous cell carcinoma cell lines. These ρ cells show sensitivity to H O . We investigated the effect of reactive oxygen species, especially the mechanism of H O -mediated cell death, in ρ cells. The up-regulation of HNE in the plasm...

BORC regulates late endosomal/lysosomal size through PIKfyve-dependent phosphatidylinositol-3,5-bisphosphate.

Mechanisms that control lysosomal function are essential for cellular homeostasis. Lysosomes adapt in size and number to cellular needs but little is known about the underlying molecular mechanism. We demonstrate that the late endosomal/lysosomal multimeric BLOC-1 related complex (BORC) regulates size of these organelles via PIKfyve-dependent phosphatidylinositol-3,5-bisphosphate [PI(3,5)P ] production. Deletion of the core BORC component Diaskedin led to increased levels of PI(3,5)P , suggesting activation...

Relationship between nucleotide-binding oligomerization domain-containing protein 2 variants and severity of acute pancreatitis.

Intestinal barrier dysfunction has been implicated in the development of infectious complications of acute pancreatitis. Nucleotide-Binding Oligomerization DomainContaining Protein 2 (NOD2) plays an important role in the proper functioning of intestinal defense mechanisms. Here, we investigated the frequency of NOD2 variants in patients with mild and severe acute pancreatitis.

Tumor suppressor p53-mediated structural reorganization of the transcriptional coactivator p300.

Transcriptional coactivator p300, a critical player in eukaryotic gene regulation, primarily functions as a histone acetyltransferase (HAT). It is also an important player in acetylation of a number of non-histone proteins, p53 being the most prominent one. Recruitment of p300 to p53 is pivotal in the regulation of p53-dependent genes. Emerging evidence suggest that p300 adopts an active conformation upon binding to the tetrameric p53, resulting in its enhanced acetylation activity. As a modular protein, p3...

Unraveling the rapid assembly process of stiff cellulosic fibers from mistletoe berries.

The mucilaginous viscin tissue within mistletoe berries possesses an extraordinary ability to be rapidly processed under ambient conditions into stiff cellulosic fibers (>14 GPa) through simple mechanical drawing. This rapid and extreme transformation process is hydration-dependent and involves an astonishing >200-fold increase in length, providing a relevant role model for efforts to produce advanced composites from cellulose-based structures such as cellulose nanocrystals (CNCs) or cellulose nanofibrils (...

Chemically engineered synthetic lipid vesicles for sensing and visualization of protein - bilayer interactions.

From pathogen intrusion to immune response, cell membrane plays an important role in signal transduction. Such signals are important for cellular proliferation and survival. However, measurement of these subtle signals through the lipid membrane scaffold is challenging. We present a chromatic model membrane vesicle system engineered to covalently bind with lysine residues of protein molecules for investigation of cellular interactions and signaling. We discovered that different protein molecules induced dif...

FadR-based biosensor assisted screening for genes enhancing fatty acyl-CoA pools in Saccharomyces cerevisiae.

Fatty acid-derived compounds have a range of industrial applications, from chemical building blocks to biofuels. Due to the highly dynamic nature of fatty acid metabolism, it is difficult to identify genes modulating fatty acyl-CoA levels using a rational approach. Metabolite biosensors can be used to screen genes from large-scale libraries in vivo in a high throughput manner. Here, a fatty acyl-CoA sensor based on the transcription factor FadR from Escherichia coli was established in Saccharomyces cerevisi...

Glyconanoparticles for Targeted Tumor Therapy of Platinum Anticancer Drug.

An important requirement to decrease the side effects of chemotherapy drugs is to develop nanocarriers with precise biological functions. In this work, a set of glyconanoparticles was prepared via self-assembly of amphiphilic glycoblock copolymers for the targeted delivery of a hydrophobic chemotherapy drug. Well-defined glycoblock copolymers that consist of 1,1-di-tert-butyl 3-(2-(metyloyloxy)ethyl)-butane-1,1,3-tricarboxylate (MAETC) together with three different protected-sugar moieties (β-D-glucopyrano...

Arginine to lysine mutations increase the aggregation stability of a single chain variable fragment through unfolded state interactions.

Increased protein solubility is known to correlate with an increase in the proportion of lysine over arginine residues. Previous work has shown that the aggregation propensity of a single-chain variable fragment (scFv) does not correlate with its conformational stability or native-state protein-protein interactions. Here we test the hypothesis that aggregation is driven by the colloidal stability of partially unfolded states, studying the behaviour of scFv mutants harbouring single or multiple site-specific...

Lewis Strength Determines Specific-Ion Effects in Aqueous and Nonaqueous Solvents.

An analysis of specific-ion effects in aqueous and nonaqueous solvents using energy decomposition analysis is presented. Specific-ion effects induce or influence physicochemical phenomena in a way that is determined by the identity of the ions present, and not merely by their charge or concentration. Such effects have been known since the seminal work of Hofmeister and are often categorized according to the well-known Hofmeister series. Examples of specific-ion effects are ubiquitous throughout chemistry an...

Switch It Inside-Out: "Schizophrenic" Behavior of All Thermoresponsive UCST-LCST Diblock Copolymers.

This feature article reviews our recent advancements on the synthesis, phase behavior, and micellar structures of diblock copolymers consisting of oppositely thermoresponsive blocks in aqueous environments. These copolymers combine a nonionic block, which shows lower critical solution temperature (LCST) behavior, with a zwitterionic block that exhibits an upper critical solution temperature (UCST). The transition temperature of the latter class of polymers is strongly controlled by its molar mass and by the...

Increased Pyruvate Dehydrogenase Activity in Skeletal Muscle of Growth Restricted Ovine Fetuses.

Fetal sheep with placental insufficiency-induced intrauterine growth restriction (IUGR) have lower fractional rates of glucose oxidation and greater gluconeogenesis, indicating lactate shuttling between skeletal muscle and liver. Suppression of pyruvate dehydrogenase (PDH) activity was proposed because of greater pyruvate dehydrogenase kinase 4 (PDK4) and pyruvate dehydrogenase kinase 1 (PDK1) mRNA concentrations in IUGR muscle. Although PDK1 and PDK4 inhibit PDH activity to reduce pyruvate metabolism, PDH ...

High-Content Screening Identifies New Inhibitors of Connexin 43 Gap Junctions.

Gap junctions (GJs) are dynamic structures composed of hexamers of connexins (Cxs), a class of transmembrane proteins enabling channel-mediated direct intercellular communication through cell-cell diffusion of ions and small metabolites. In defined conditions, Cxs also work as hemichannels allowing exchanges between the cytoplasm and the extracellular medium. The most common GJ channel is formed by connexin 43 (Cx43) and plays an important role in physiological and pathological processes in excitable tissue...

The developmentally regulated fetal enterocyte gene, ZP4, mediates anti-inflammation by the symbiotic bacterial surface factor polysaccharide A on B. fragilis.

Initial colonizing bacteria play a critical role in completing the development of the immune system in the gastrointestinal tract of infants. Yet, the interaction of colonizing bacterial organisms with the developing human intestine favors inflammation over immune homeostasis. This characteristic of bacterial-intestinal interaction contributes to the pathogenesis of necrotizing enterocolitis (NEC), a devastating premature infant intestinal inflammatory disease. However, paradoxically unique pioneer bacteria...

Overexpression of Aurora-A bypasses cytokinesis through phosphorylation of SLAN.

Mitosis is a complicated process by which eukaryotic cells segregate duplicated genomes into two daughter cells. To achieve the goal, numerous regulators have been revealed to control mitosis. The oncogenic Aurora-A is a versatile kinase responsible for the regulation of mitosis including chromosome condensation, spindle assembly and centrosome maturation through phosphorylating a range of substrates. However, overexpression of Aurora-A bypasses cytokinesis, thereby generating multiple nuclei without knowin...

Integrin Signaling: Linking Mechanical Stimulation to Skeletal Muscle Hypertrophy.

The α7β1 integrin is a transmembrane adhesion protein that connects laminin in the extracellular matrix (ECM) with actin in skeletal muscle fibers. The α7β1 integrin is highly expressed in skeletal muscle and is concentrated at costameres and myotendious junctions, providing the opportunity to transmit longitudinal and lateral forces across the membrane. Studies have demonstrated that α7 integrin subunit mRNA and protein are upregulated following eccentric contractions as a mechanism to reinforce load ...

Downregulated Long Noncoding RNA GAS5 Fails to Function as Decoy of CEBPB, Resulting in Increased GDF15 Expression and Rapid Ovarian Cancer Cell Proliferation.

Growth differentiation factor 15 (GDF15), a newly identified member of transforming growth factor (GDF) superfamily, is upregulated in ovarian (OV) cancer. Upregulated GDF15 positively correlates with poor prognosis of OV cancer. Thus, elucidation of the mechanism underlying GDF15 overexpression is important. PROMO and JASPAR prediction software were used to find transcription factors for GDF15 expression. Data from TCGA database were analyzed to find long noncoding RNAs (lncRNAs) that were also abnormally...

modulates X-box-binding protein 1 (XBP1) expression during the adaptive phase of the unfolded protein response.

During endoplasmic reticulum (ER) stress conditions, an adaptive signaling network termed the unfolded protein response (UPR) is activated. The UPR's function is to increase ER protein-folding capacity in order to attenuate ER stress, restore ER homeostasis, and, most importantly, promote cell survival. X-box-binding protein 1 (XBP1) is one component of the UPR and is a proadaptive transcription factor that is subject to transcriptional, post-transcriptional, and post-translational control. In the present s...


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