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PubMed Journals Articles About "Aushon BioSystems" RSS

16:43 EDT 26th March 2019 | BioPortfolio

Aushon BioSystems PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest Aushon BioSystems articles that have been published worldwide.

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Showing "Aushon BioSystems" PubMed Articles 1–25 of 43

Hydrodynamics and mathematical modelling in a low HRT inverse fluidized-bed reactor for biological sulphate reduction.

Biological reduction of sulphate at low hydraulic retention time (HRT) is presented in this paper. A sulphidogenic inverse fluidized-bed bioreactor (IFBB) was operated successfully at a progressively decreasing HRT from 1 to 0.125 days for a total of 155 days. Synthetic wastewater containing sulphate at a concentration of 745 (± 17) mg/L was used. COD was supplied as lactate in variable concentrations at COD/SO ratios of 1.2-2.4. The pH of the feed ranged between 5.2 and 6.2. The highest measured remo...


A highly specific and ultrasensitive p-aminophenylether-based fluorescent probe for imaging native HOCl in live cells and zebrafish.

It is very important to detect native hypochlorous acid (HOCl) in the complex biosystems owing to the important roles of HOCl in the immune defense and the pathogenesis of numerous diseases. In this paper, a new p-aminophenylether-based fluorescent probe PAPE-HA was developed for specific detection of HOCl. Probe PAPE-HA could implement the quantitative detection of HOCl ranging from 0 to 1 μM and the detection limit was obtained as low as 1.37 nM. Additionally, probe PAPE-HA could reach a rapid respon...

STRmix™ collaborative exercise on DNA mixture interpretation.

An intra and inter-laboratory study using the probabilistic genotyping (PG) software STRmix™ is reported. Two complex mixtures from the PROVEDIt set, analysed on an Applied Biosystems™ 3500 Series Genetic Analyzer, were selected. 174 participants responded. For Sample 1 (low template, in the order of 200 rfu for major contributors) five participants described the comparison as inconclusive with respect to the POI or excluded him. Where LRs were assigned, the point estimates ranging from 2 × 10 to 8...


Acetone, butanol, and ethanol production from the green seaweed Enteromorpha intestinalis via the separate hydrolysis and fermentation.

Acetone, butanol, and ethanol (ABE) were produced following the separate hydrolysis and fermentation (SHF) method using polysaccharides from the green macroalgae Enteromorpha intestinalis as biomass. We focused on the optimization of enzymatic saccharification as pretreatments for the fermentation of E. intestinalis. Pretreatment was carried out with 10% (w/v) seaweed slurry and 270-mM HSO at 121 °C for 60 min. Monosaccharides (mainly glucose) were obtained from enzymatic hydrolysis with a 16-U/mL mixtur...

Production of cutinase by solid-state fermentation and its use as adjuvant in bioherbicide formulation.

In the present study, it was presented a strategy to maximize the cutinase production by solid-state fermentation from different microorganisms and substrates. The best results were observed using Fusarium verticillioides, rice bran being the main substrate. Maximum yield of cutinase obtained by the strain was 16.22 U/g. For concentration, ethanol precipitation was used, and the purification factor was 2.4. The optimum temperature and pH for enzyme activity were 35 °C and 6.5, respectively. The enzyme wa...

Simultaneous discrimination of Cys, Hcy, GSH and H2S in living cells through a multi-signal combination strategy.

Biothiols are essential reactive sulfur species (RSS), which play crucial roles in various critical physiological activities. To clarify their complex correlations in signal transduction and metabolism pathways, methods to distinguish H2S, Cys, Hcy, and GSH at the same time are highly needed. Herein, we deliberately integrate 7-diethylcoumarin and resorufin through an ether bond to develop RC for simultaneous differentiation of Cys, Hcy, GSH, and H2S for the first time. RC displays distinct fluorescence col...

Bacterial indoleacetic acid-induced synthesis of colloidal AgO nanocrystals and their biological activities.

The biosynthesis and biological activity of colloidal AgO nanocrystals have not been well studied, although they have potential applications in many fields. For the first time, we developed a reducing agent free, cost-effective technique for AgO biosynthesis using Xanthomonas sp. P5. The optimal conditions for AgO synthesis were 50 °C, pH 8, and 2.5 mM AgNO. Using these conditions the yield of AgO obtained at 10 h was about five times higher than that obtained at 12 h under unoptimized conditions. AgO ...

A dicyclic-type electrode-based biofilm reactor for simultaneous nitrate and Cr(VI) reduction.

A dicyclic-type electrode-based biofilm-electrode reactor (BER) was investigated for simultaneous removal of nitrate and Cr(VI). In the absence of Cr(VI), almost complete denitrification of 50 mg/L NO-N was achieved at a very low C/N ratio of 0.8 with the optimal current of 50 mA. Cr(VI) was removed by biological reduction and co-precipitation when Cr(VI) was taken as the only electron acceptor, and the removal efficiencies of Cr(VI) were 99.8%. In the coexistent system of nitrate and Cr(VI), nitrate remo...

Startup strategy for nitrogen removal via nitrite in a BAF system.

A biological aerated filter (BAF) pilot plant consisting of two reactors (aerobic and anoxic one) was used to determine a strategy to remove nitrogen via nitrite. RNA/DNA analysis was performed to assess microbial activity and support chemical results. In less than 13 days the pilot plant was able to remove COD and suspended solids. Nitrogen removal via nitrite pathway could not be observed until day 130 when the empty bed contact time (EBCT) was set at 0.71 h. Nitrite was detected in the aerated BAF efflu...

Lipid production by Cryptococcus albidus using biowastes hydrolysed by indigenous microbes.

The efficiency of Cryptococcusalbidus was evaluated for its abilities to assimilate onion and apple hydrolysates as a medium for lipid production. Onion waste (OW) and apple waste (AW) were hydrolysed at an organic load of 2% total solids by indigenous microbes under mesophilic conditions. The indigenous microbes effectively hydrolysed both wastes giving the highest reducing sugar content of 4.8 g/L and 10.8 g/L with OW and AW hydrolysates, respectively. The microbiome analysis revealed that most of the i...

Cellulolytic enzyme production from agricultural residues for biofuel purpose on circular economy approach.

This study evaluated the production of cellulolytic enzymes from different agricultural residues. The crude enzyme extract produced was characterized and applied for saccharification of some agricultural residues. Maximum cellulolytic activities were obtained using soybean hulls. All enzymatic activities were highly stable at 40 °C at a pH range of 4.5-5.5. For stability at low temperatures, the enzyme extract was stored at freezing temperature and cooling for about 290 days without major loss of activity...

A novel osmotic pressure strategy to improve erythritol production by Yarrowia lipolytica from glycerol.

Polyethylene glycol (PEG) is polymer that was used to replace NaCl (reference media) as an osmotic stress agent for the synthesis of erythritol by the osmophilic yeast Yarrowia lipolytica. Two strains, the wild-type strain IMUFRJ 50682 and the lab strain W29, were grown in the presence of PEG of different molecular weights. For strain IMUFRJ 50682, the erythritol titer was increased by 40% in the presence of PEG2000 as compared to the reference media (with NaCl). A similar increase was also observed for str...

Integrated thermophilic enzyme-immobilized reactor and high-rate biological reactors for treatment of palm oil-containing wastewater without sludge production.

Bacillus pumilus, the thermophilic microorganism, was used to biosynthesise lipase of specific activity 5173 U. The purified lipase was highly stable in the pH range from 1 to 7 and temperature at 50 °C. The functionalized nanoporous activated carbon matrix was used for the immobilization of lipase at the optimum conditions and was used for the hydrolysis of palm oil-containing wastewater at optimum time, 3 h, pH 7, and at temperature 50 °C. The hydrolyzed palm oil wastewater was treated in an upflow a...

Enzymatic synthesis of cefazolin using immobilized recombinant cephalosporin-acid synthetase as the biocatalyst.

A method for the synthesis of β-lactam antibiotic cefazolin (CEZ) by enzymatic acylation of 7-amino-3-(5-methyl-l,3,4-thiadiazol-2-yl)thiomethyl-3-cephem-4-carboxylic acid (TDA) using immobilized cephalosporin-acid synthetase (IECASA) from recombinant E. coli strain VKPM B-12316 has been developed. A stepwise pH gradient designed on the basis of investigations on the solubility of components was applied for synthesis. This helped in avoiding the precipitation of TDA in the reaction when its initial concent...

Outdoor microalgae cultivation in airlift photobioreactor at high irradiance and temperature conditions: effect of batch and fed-batch strategies, photoinhibition, and temperature stress.

The microalgae Scenedesmus abundans cultivated in five identical airlift photobioreactors (PBRs) in batch and fed-batch modes at the outdoor tropical condition. The microalgae strain S. abundans was found to tolerate high temperature (35-45 °C) and high light intensity (770-1690 µmol m s). The highest biomass productivities were 152.5-162.5 mg L day for fed-batch strategy. The biomass productivity was drastically reduced due to photoinhibition effect at a culture temperature of > 45 °C. The lip...

Preparation of a near-infrared fluorescent probe based on IR-780 for highly selective and sensitive detection of bisulfite/sulfite in food, living cells and mice.

A near-infrared fluorescence probe, was designed and synthesized for the detection of bisulfite/sulfite in food, living HeLa cells and mice. The probe is a stable hemicyanine skeleton based on IR-780, which produces a distinct color and fluorescence change before and after reaction with bisulfite, and shows excellent analytical performance such as high sensitivity with a detection limit of 0.37 μM, high selectivity toward bisulfite over other substances. More importantly, the probe determined bisulfite in ...

Emerging techniques for cell disruption and extraction of valuable bio-molecules of microalgae Nannochloropsis sp.

Microalgae of Nannochloropsis sp. present valuable source of bio-molecules (pigments, lipids, proteins) that have nutritional potential for the prevention and treatment of human diseases. Moreover, some species of Nannochloropsis are the promising sources of biofuels and excellent candidates for the replacement of classical biofuel crops. This review describes and compares the efficiency of different conventional and novel techniques that can be used for cell disruption and recovery of bio-molecules from N...

Optimization of microbial cell disruption using pressurized CO for improving lipid recovery from wet biomass.

Microbial cell disruption using pressurized gases (e.g., CO) is a promising approach to improve the lipid recovery from wet oleaginous microorganisms by eliminating the energy-intensive drying required for conventional methods. In this study, we perform cell disruption of Rhodotorula glutinis using pressurized CH, N, and Ar where we find the efficacy of these gases on cell viability is minimal. Since CO is found to be the only viable gas for microbial cell disruption among these four gases, we use a combina...

Interpretation of the effect of CYP2C9, VKORC1 and CYP4F2 variants on warfarin dosing adjustment in Turkey.

It was aimed to underline the importance and explain the meaning of genetic testing in warfarin dosing and investigate and evaluate the contributions of the CYP2C9, VKORC1, and CYP4F2 variants in a Turkish population. Two hundred patients were genotyped for CYP2C9 (rs1799853, rs1057910 and rs56165452), VKORC1 (rs9934438, rs8050894, rs9923231, rs7294 and rs2359612) and CYP4F2 (rs2108622), yet, only 127 patients were found suitable for further evaluation in terms of their personal response to warfarin due to ...

Potential of in situ SSF laccase produced from Ganoderma lucidum RCK 2011 in biobleaching of paper pulp.

Production of laccase from Ganoderma lucidum RCK 2011 under solid-state fermentation (SSF) conditions was optimized using response surface methodology, resulting in an approximate eightfold increase compared to that in the unoptimized media. Further, the enzyme produced under SSF as whole fermented substrate (in situ SSF laccase) was found to be more stable than the in vitro enzyme (harvested by downstreaming processing of fermented wheat bran). Interestingly, the biobleaching potentials of both in situ and...

Microbial Ligninolysis: Toward a Bottom-Up Approach for Lignin Upgrading.

Lignin, an underutilized source of biomass, is a challenging target for decomposition by both biological and nonbiological approaches. As lignin accounts for 15-40% of the dry weight of lignocellulose, its conversion to valued chemicals is considered promising. Although depolymerization of the complicated lignin structures is still not fully understood, biochemical reactions for various lignin-derived compounds, especially dimeric and monomeric aromatics, have been characterized in some fungi and bacteria k...

Ethanol production from xylose is highly increased by the Kluyveromyces marxianus mutant 17694-DH1.

Directed evolutionary approach and random mutagenesis were performed on thermotolerant yeast Kluyveromyces marxianus KCTC17694 for isolating a yeast strain producing ethanol from xylose efficiently. The isolated mutant strain, K. marxianus 17694-DH1, showed 290% and 131% improvement in ethanol concentration and ethanol production yield from xylose, respectively, as compared with the parental strain. Sequencing of the KmXYL1 gene of K. marxianus 17694-DH1 revealed substitutions of arginine and tryptophan wit...

Stereolithographic 4D Bioprinting of Multiresponsive Architectures for Neural Engineering.

4D printing represents one of the most advanced fabrication techniques for prospective applications in tissue engineering, biomedical devices, and soft robotics, among others. In this study, a novel multiresponsive architecture is developed through stereolithography-based 4D printing, where a universal concept of stress-induced shape transformation is applied to achieve the 4D reprogramming. The light-induced graded internal stress followed by a subsequent solvent-induced relaxation, driving an autonomous a...

Talking to cells: semiconductor nanomaterials at the cellular interface.

The interface of biological components with semiconductors is a growing field with numerous applications. For example, the interfaces can be used to sense and modulate the electrical activity of single cells and tissues. From the materials point of view, silicon is the ideal option for such studies due to its controlled chemical synthesis, scalable lithography for functional devices, excellent electronic and optical properties, biocompatibility and biodegradability. Recent advances in this area are pushing ...

Application of process system engineering tools to the fed-batch production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from a vinasses-molasses Mixture.

Fed-batch production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer using vinasses-molasses mixture is carried out in this work by implementing different process systems engineering tools. Two fed-batch strategies are tested experimentally at 5 L scale, considering only offline information: (1) offline optimizing control and (2) exponential feeding. Application of these strategies showed that different feeding profiles result in different dynamic behaviour, influencing both, yield and biopolymer...


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