Topics

PubMed Journals Articles About "High Resolution Scanning Optimal Biosensor Reporter Promoters Yeast" RSS

04:46 EST 26th February 2020 | BioPortfolio

High Resolution Scanning Optimal Biosensor Reporter Promoters Yeast PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest High Resolution Scanning Optimal Biosensor Reporter Promoters Yeast articles that have been published worldwide.

More Information about "High Resolution Scanning Optimal Biosensor Reporter Promoters Yeast" on BioPortfolio

We have published hundreds of High Resolution Scanning Optimal Biosensor Reporter Promoters Yeast news stories on BioPortfolio along with dozens of High Resolution Scanning Optimal Biosensor Reporter Promoters Yeast Clinical Trials and PubMed Articles about High Resolution Scanning Optimal Biosensor Reporter Promoters Yeast for you to read. In addition to the medical data, news and clinical trials, BioPortfolio also has a large collection of High Resolution Scanning Optimal Biosensor Reporter Promoters Yeast Companies in our database. You can also find out about relevant High Resolution Scanning Optimal Biosensor Reporter Promoters Yeast Drugs and Medications on this site too.

Showing "High resolution scanning optimal biosensor reporter promoters yeast" PubMed Articles 1–25 of 38,000+

High-resolution scanning of optimal biosensor reporter promoters in yeast.

Small-molecule binding allosteric transcription factors (aTFs) derived from bacteria enable real-time monitoring of metabolite abundances, high-throughput screening of genetic designs, and dynamic control of metabolism. Yet, engineering of reporter promoter designs of prokaryotic aTF biosensors in eukaryotic cells is complex. Here we investigate the impact of aTF binding site positions at single-nucleotide resolution in >300 reporter promoter designs in Saccharomyces cerevisiae. From this we identify biosen...


Expanding the dynamic range of a transcription factor-based biosensor in Saccharomyces cerevisiae.

Metabolite biosensors are useful tools for high-throughput screening- and pathway regulation approaches. An important feature of biosensors is the dynamic range. To expand the maximum dynamic range of a transcription factor-based biosensor in Saccharomyces cerevisiae, using the fapO/FapR system from Bacillus subtilis as an example case, five native promoters, including constitutive and glucose-regulated ones, were modified. By evaluating different binding site (BS) positions in the core promoters, we identi...

Identification and characterization of ethanol inducible promoters of Zymomonas mobilis based on omics data and dual reporter-gene system.

Zymomonas mobilis is a model bacterial ethanologen, and has been engineered to produce lignocellulosic biofuels and biochemicals such as 2,3-butanediol. We have previously identified promoters of different strengths using systems biology datasets, and characterized them using the flow cytometry-based dual reporter-gene system. Here, we further demonstrated the capability of applying the dual reporter-gene system and omics datasets on discovering inducible promoters. Ten candidate ethanol inducible promoters...


A novel mıcrobıal bıosensor system based on C. tropicalis yeast cells for selectıve determınatıon of L-Ascorbıc acid.

In this study, a novel microbial biosensor was developed for the selective determination of L-Ascorbic acid. In the construction of the microbial biosensor, lyophilized Candida tropicalis yeast cells were immobilized with o-aminophenol by forming a film layer on a platinum electrode surface using electropolymerization. L-Ascorbic acid was quantified on the basis of both amperometric and differential pulse voltammetry (DPV) methods using the biosensor. The measurements were made at +0.24 V (vs Ag/AgCl) for ...

Promoter screening facilitates heterologous production of complex secondary metabolites in Burkholderiales strains.

Burkholderiales are an emerging source of bioactive secondary metabolites and have the potential to be a robust chassis for metabolites from Gram-negative bacteria. However, only few constitutive promoters can be utilized in Burkholderiales. Herein, we described the screening of strong constitutive promoters from Burkholderiales strain DSM 7029, and 37 promoters identified from transcriptome sequencing were cloned and characterized using a firefly luciferase reporter and were further verified by qPCR analys...

Biosensors Design in Yeast and Applications in Metabolic Engineering.

Engineering microbial cell factories is a potential approach of sustainable production of chemicals, fuels and pharmaceuticals. However, testing the production of molecules in high throughput is still a time-consuming and laborious process since product synthesis usually does not confer a clear phenotype. Therefore, it is necessary to develop new techniques for fast high-producer screening. Genetically encoded biosensors are considered to be promising devices for high-throughput analysis owing to their abil...

Improved detection of molecularly targeted iron oxide particles in mouse brain using B field stabilised high resolution MRI.

High resolution multi-gradient echo (MGE) scanning is typically used for detection of molecularly targeted iron oxide particles. The images of individual echoes are often combined to generate a composite image with improved SNR from the early echoes and boosted contrast from later echoes. In 3D implementations prolonged scanning at high gradient duty cycles induces a B shift that predominantly affects image alignment in the slow phase encoding dimension of 3D MGE images. The effect corrupts the composite ec...

Validation of structural brain connectivity networks: The impact of scanning parameters.

Evaluation of the structural connectivity (SC) of the brain based on tractography has mainly focused on the choice of diffusion model, tractography algorithm, and their respective parameter settings. Here, we systematically validate SC derived from a post mortem monkey brain, while varying key acquisition parameters such as the b-value, gradient angular resolution and image resolution. As gold standard we use the connectivity matrix obtained invasively with histological tracers by Markov et al. (2014). As p...

Asparagine-linked glycosylation is not directly coupled to protein translocation across the endoplasmic reticulum in .

Mammalian cells express two oligosaccharyltransferase complexes (STT3A and STT3B) that have distinct roles in N-linked glycosylation. The STT3A complex interacts directly with the protein translocation channel to mediate glycosylation of proteins using an N-terminal to C-terminal scanning mechanism. N-linked glycosylation of proteins in budding yeast has been assumed to be a cotranslational reaction. We have compared glycosylation of several glycoproteins in yeast and mammalian cells. Prosaposin, a cysteine...

Electrochemical xanthine detection by enzymatic method based on Ag doped ZnO nanoparticles by using polypyrrole.

A sensitive electrochemical detection of xanthine (X), which is an early biomarker of fish meat spoilage, was achieved by a novel biosensor developed via three main steps. The first step is the electropolymerization of a conducting polymer (pyrrole) onto the pencil graphite electrode (PGE). The second step is the entrapment of silver-doped zinc oxide nanoparticles (nano Ag-ZnO) onto PGE, which has already been doped with polypyrrole (PPy). The third step is the immobilization of the enzyme (xanthine oxidase...

Design and characterization of biosensors for the screening of modular assembled naringenin biosynthetic library in Saccharomyces cerevisiae.

A common challenge in the assembly and optimization of plant natural product biosynthetic pathways in recombinant hosts is the identification of gene orthologues that will result in best production titers. Here, we describe the modular assembly of a naringenin biosynthetic pathways in Saccharomyces cerevisiae that was facilitated by optimized naringenin-inducible prokaryotic transcription activators used as biosensors. The biosensors were designed and developed in S. cerevisiae by a multiparametric engineer...

Cryoelectron Microscopy Structure of a Yeast Centromeric Nucleosome at 2.7 Å Resolution.

Kinetochores mediate chromosome segregation during cell division. They assemble on centromeric nucleosomes and capture spindle microtubules. In budding yeast, a kinetochore links a single nucleosome, containing the histone variant Cse4 instead of H3, with a single microtubule. Conservation of most kinetochore components from yeast to metazoans suggests that the yeast kinetochore represents a module of the more complex metazoan arrangements. We describe here a streamlined protocol for reconstituting a yeast ...

High-performance electrochemical enzyme sensor for organophosphate pesticide detection using modified metal-organic framework sensing platforms.

A practical electrochemical biosensor with high sensitivity was developed for detecting organophosphorus (OP). Initially, Ce metal was introduced into an UiO-66-template to form Ce/UiO-66. Later, graphene oxide (GO), carbon black (CB) and multi-walled carbon nanotubes (MWCNTs) were separately added to Ce/UiO-66 to compare the effect of different carbon-based material types on the performance of the biosensor. Exclusively, Ce/UiO-66/MWCNTs with a Ce (7%) and MWCNT (30%) matrix was found to not only load more...

Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide.

Bacillus subtilis has been extensively utilized as a host to express heterologous protein used in various industrial processes. Hence, the secretory overproduction of recombinant proteins is highly dependent on the strong promoters and signal peptides with high efficiency in B. subtilis. To enlarge the limited information of signal peptides and promoters suitable for specific proteins expression at a high-level, a series of plasmids carrying various signal peptides and single, dual, or triple promoters were...

TMTpro: Design, synthesis and initial evaluation of a Proline-based isobaric 16-plex Tandem Mass Tag reagent set.

The design and synthesis of a proline-based reporter isobaric Tandem Mass Tag structure (TMTpro) is presented. An analysis is made of the performance of the new TMTpro tags in comparison with the current commercially available dimethylpiperidine-reporter-based TMT10/11 reagents. The new reporter structure provides a set of 16 tags for use with resolution of 6.3 mDa mass differences in high resolution mass spectrometers and a set of 9 reagents with 1 Da spacing between reporter ions for single Dalton analysi...

A Fast and Easily Parallelizable Biosensor Method for Measuring Extractable Tetracyclines in Soils.

Quantification of extractable antibiotics in soils is important to assessing their bioavailability and mobility, and ultimately their ecotoxicological and health risks. This study aimed to establish a biosensor method for detecting extractable tetracyclines in soils (Alfisol, Mollisol, and Ultisol) using whole-cell biosensors containing a reporter plasmid (pMTGFP or pMTmCherry) carrying fluorescent protein genes tightly controlled by tetracyclines-responsive control region (tetRO). This whole-cell biosensor...

Improving the imaging capability of an on-axis transmission Kikuchi detector.

Transmission Kikuchi Diffraction (TKD) in the scanning electron microscope has been developing at a fast pace since its introduction less than a decade ago. The recently presented on-axis detector configuration, with its optimized geometry, has significantly increased the signal yield and facilitated the acquisition of STEM images in bright field (BF) and dark field (DF) mode, in addition to the automated orientation mapping of nanocrystalline electron transparent samples. However, the physical position of ...

Development of an in vivo fluorescence based gene expression reporter system for Clostridium tyrobutyricum.

C. tyrobutyricum, an acidogenic Clostridium, has aroused increasing interest due to its potential to produce biofuel efficiently. However, construction of recombinant C. tyrobutyricum for enhanced biofuel production has been impeded by the limited genetic engineering tools. In this study, a flavin mononucleotide (FMN)-dependent fluorescent protein Bs2-based gene expression reporter system was developed to monitor transformation and explore in vivo strength and regulation of various promoters in C. tyrobutyr...

High resolution time-of-flight MR-angiography at 7 T exploiting VERSE saturation, compressed sensing and segmentation.

3D Time-of-Flight (TOF) MR-angiography (MRA) substantially benefits from ultra-high magnetic field strengths (≥7 T) due to increased Signal-to-Noise ratio and improved contrast. However, high-resolution TOF-MRA usually requires long acquisition times. In addition, specific absorption rate constraints limit the choice of optimal pulse sequence parameters, especially if venous saturation is employed.

Acoustic-resolution-based photoacoustic microscopy with non-coaxial arrangements and a multiple vertical scan for high lateral resolution in-depth.

In conventional acoustic-resolution-based photoacoustic microscopy (ARPAM), a focused ultrasound transducer is placed coaxially with the laser beam to obtain the generated ultrasound signals. The information from deep regions can be greatly affected by the shallow targets. More importantly, in ARPAM the irreconcilable conflict between the lateral resolution and depth of fields has always been a major factor that lowers the imaging quality. In this work, an ARPAM system was developed, in which a non-coaxial ...

The kinetic analysis of breast cancer: An investigation of the optimal temporal resolution for dynamic contrast-enhanced MR imaging.

There is wide agreement that morphologic features and enhancement kinetics should be evaluated for MRI of the breast, although there has been no clear consensus concerning optimal temporal resolutions. The objective of this study was to investigate the optimal temporal resolution for the kinetic analysis of breast cancers.

Amperometric Biosensor Based on Cu2O@Au Nanocomposites for the Detection of Galectin-1 via Lactose-Galectin Interactions.

In this work, a novel label-free electrochemical biosensor is developed for the detection of galectin-1 (Gal-1) based on the gold nanoparticles (AuNPs) loaded octahedral Cu2O (Cu2O@Au) nanocomposites. The AuNPs on the surface of Cu2O nanocrystals not only enhance the electrochemical performance, but also can serve as the binding sites for lactose ligand which can specifically bind with Gal-1. The Cu2O@Au nanocomposites provide the synergic effect of electrochemical signal amplification and lactose-galectin ...

Ultrasound imaging of gene expression in mammalian cells.

The study of cellular processes occurring inside intact organisms requires methods to visualize cellular functions such as gene expression in deep tissues. Ultrasound is a widely used biomedical technology enabling noninvasive imaging with high spatial and temporal resolution. However, no genetically encoded molecular reporters are available to connect ultrasound contrast to gene expression in mammalian cells. To address this limitation, we introduce mammalian acoustic reporter genes. Starting with a gene c...

Improvement of ,-Muconic Acid Production in through Biosensor-Aided Genome Engineering.

Muconic acid is a potential platform chemical for the production of nylon, polyurethanes, and terephthalic acid. It is also an attractive functional copolymer in plastics due to its two double bonds. At this time, no economically viable process for the production of muconic acid exists. To harness novel genetic targets for improved production of -muconic acid (CCM) in the yeast , we employed a CCM-biosensor coupled to GFP expression with a broad dynamic response to screen UV-mutagenesis libraries of CCM-pro...

Red Light/Green Light, a Dual Fluorescent Protein Reporter System To Study Enhancer-Promoter Specificity in .

Enhancers activate gene transcription in spatial and temporal patterns by interactions with gene promoters. These elements typically reside distal to their target promoter, with which they must interact selectively. Additional elements may contribute to enhancer-promoter specificity, including remote control element sequences within enhancers, tethering elements near promoters, and insulator/boundary elements that disrupt off-target interactions. However, few of these elements have been mapped, and as a res...


Quick Search