Advertisement

Topics

PubMed Journals Articles About "Phenotypic Genotypic Study Antimicrobial Resistance Patterns Coli Isolates" RSS

09:10 EDT 20th June 2019 | BioPortfolio

Phenotypic Genotypic Study Antimicrobial Resistance Patterns Coli Isolates PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest Phenotypic Genotypic Study Antimicrobial Resistance Patterns Coli Isolates articles that have been published worldwide.

More Information about "Phenotypic Genotypic Study Antimicrobial Resistance Patterns Coli Isolates" on BioPortfolio

We have published hundreds of Phenotypic Genotypic Study Antimicrobial Resistance Patterns Coli Isolates news stories on BioPortfolio along with dozens of Phenotypic Genotypic Study Antimicrobial Resistance Patterns Coli Isolates Clinical Trials and PubMed Articles about Phenotypic Genotypic Study Antimicrobial Resistance Patterns Coli Isolates for you to read. In addition to the medical data, news and clinical trials, BioPortfolio also has a large collection of Phenotypic Genotypic Study Antimicrobial Resistance Patterns Coli Isolates Companies in our database. You can also find out about relevant Phenotypic Genotypic Study Antimicrobial Resistance Patterns Coli Isolates Drugs and Medications on this site too.

Showing "Phenotypic genotypic study antimicrobial resistance patterns coli isolates" PubMed Articles 1–25 of 69,000+

Phenotypic and genotypic study on antimicrobial resistance patterns of E. coli isolates from bovine mastitis.

It is important to evaluate the antimicrobial resistance pattern by genotypic and phenotypic methods in epidemiological studies in order to control antimicrobial resistance and to improve the outcome of the treatments. Four-hundred and thirty clinical mastitis samples were collected from 14 dairy herds in five different cities. The antimicrobial susceptibility test was performed using agar disk diffusion for 70 identified Escherichia coli isolates. The antimicrobial resistance genes including strA, strB, aa...


Genotypic antimicrobial resistance assays for use on E. coli isolates and stool specimens.

Antimicrobial resistance (AMR) is an emerging public health problem and methods for surveillance are needed. We designed 85 sequence-specific PCR reactions to detect 79 genes or mutations associated with resistance across 10 major antimicrobial classes, with a focus on E. coli. The 85 qPCR assays demonstrated >99.9% concordance with sequencing. We evaluated the correlation between genotypic resistance markers and phenotypic susceptibility results on 239 E. coli isolates. Both sensitivity and specificity exc...

Emergence of CMY-2-Producing Escherichia coli in Korean Layer Parent Stock.

The use of antimicrobials in food animals is the major determinant for the propagation of resistant bacteria in the animal reservoir. Especially, parent stock (PS) produces thousands of eggs for commercial laying hens and can transfer a variety of viral and bacterial agents to chicks. The objective of this study was to investigate the presence and distribution of third-generation cephalosporin-resistant and extended-spectrum β-lactamase (ESBL)- and plasmid-mediated AmpC (pAmpC)-producing Escherichia coli i...


Phenotypic and Genotypic characterization of ESBLs, AmpC and Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli Isolates.

Drug resistance among gram-negative bacteria is a worldwide challenge. Due to the importance of drug-resistant Klebsiella pneumoniae and Escherichia coli strains in hospital-acquired infections, we aimed to determine the phenotypic and genotypic characteristics of ESBLs, AmpC and Carbapenemase-producing isolates, obtained from hospitalized patients in Tehran and Ilam, Iran.

Genotypic and Phenotypic Variations in Botrytis spp. Isolates from Single Strawberry Flowers.

Gray mold, caused by Botrytis spp., is among the most devastating diseases affecting strawberry worldwide. The great diversity present in the pathogen enhances its ability to survive and adapt in the field. In this study, we explored the genotypic and phenotypic diversity present in single strawberry flowers. In total, 192 isolates were collected from 19 flowers and four farms, and 9 to 12 isolates were collected from each flower. Forty-two haplotypes were found using microsatellite fragment analysis. Multi...

High prevalence of vancomycin and high-level gentamicin resistance in Enterococcus faecalis isolates.

Multiple drug-resistant enterococci are major cause of healthcare-associated infections due to their antibiotic resistance traits. Among them, Enterococcus faecalis is an important opportunistic pathogen causing various hospital-acquired infections. A total of 53 E. faecalis isolates were obtained from various infections. They were identified by phenotypic and genotypic methods. Determination of antimicrobial resistance patterns was done according to CLSI guidelines. The isolates that were non-susceptible t...

Phenotypic and Genotypic Characterization of Extended-Spectrum β-Lactamase-Producing Enterobacteriaceae in Asymptomatic Bacteriuria in Pregnancy.

Asymptomatic bacteriuria (ASB) has been consistently observed in pregnancy. However, there is a paucity of data on the prevalence and characteristics of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae in ASB in pregnant women. Therefore, we sought to investigate ESBL-producing and multidrug-resistant Enterobacteriaceae in antenatal women with ASB. Urine samples were collected from 310 asymptomatic pregnant women attending primary antenatal clinics and screened for significant bacteriuria....

Detection of plasmid-mediated quinolone resistance genes in β-lactamase-producing Escherichia coli isolates from layer hens.

This study was conducted to investigate the presence of plasmid-mediated quinolone resistance (PMQR) genes in β-lactamase-producing Escherichia coli isolates from layer hens and to characterize their molecular background. Among 142 E. coli isolates, 86 (60.6%) showed multidrug resistance and 15 (10.6%) were found to be β-lactamase-producing E. coli. Extended-spectrum β-lactamase (ESBL) and plasmid-mediated AmpC (pAmpC) β-lactamase genes, blaCTX-M-14 and blaCMY-2, were identified in three and six E. coli...

Genotypic and phenotypic profiles of virulence factors and antimicrobial resistance of Proteus mirabilis isolated from chicken carcasses: potential zoonotic risk.

Proteus mirabilis is an opportunistic pathogen often associated with a variety of human infections acquired both in the community and in hospitals. In this context, the present work aimed to evaluate the genotypic and phenotypic characteristics of the virulence factors and antimicrobial resistance determinants of 32 P. mirabilis strains isolated from chicken carcasses in a poultry slaughterhouse in the north of the state of Paraná, Brazil, in order to assess a potential zoonotic risk. The isolates presente...

Genetic mutations at the quinolone resistance-determining region are related to changes in the epidemiological profile of methicillin-resistant Staphylococcus aureus isolates.

Methicillin-resistant Staphylococcus aureus (MRSA) is an important agent of nosocomial infections, and mutations at the Quinolone Resistance-Determining Region (QRDR) of gyr and par genes have been described among them. This study aimed to characterize phenotypic and genotypic resistance for fluoroquinolones in 69 MRSA isolates of different clonal lineages from hospitals in Rio de Janeiro, Brazil.

Population Structure and Antimicrobial Resistance Traits of Avian-origin mcr-1-positive Escherichia coli in Eastern China, 2015 to 2017.

Recent emergence of mcr-1-positive E. coli (MCRPEC) is causing serious concern around the world. Due to poultry-origin E. coli holding zoonotic potential, the improved understandings of MCRPEC population structure and antimicrobial resistance are critical to public health purposes. This study provided novel insights into the molecular epidemiology of avian-origin MCRPEC. For the mcr genes prevalence study, we analyzed 1360 E. coli recovered from avian colibacillosis in eastern China from 2015 to 2017. The m...

Multidrug resistance of Salmonella infantis in Peru: a study through next generation sequencing.

To describe the phenotypic and genotypic patterns of the antimicrobial resistance of Salmonella Infantis in Peru.

Epidemiology and antibiotic resistance trends in clinical isolates of Pseudomonas aeruginosa from Rio de janeiro - Brazil: Importance of mutational mechanisms over the years (1995-2015).

Pseudomonas aeruginosa is a major health concern globally and treating infections caused by MDR-isolates unarguably a humongous challenge that remains an unmet need in modern medicine. To determine patterns and mechanisms of antimicrobial resistance and its spread over the years in Rio de Janeiro, Brazil, 88 P. aeruginosa isolates were selected from 1995 to 2015. Phenotypic and genotypic characterization of antimicrobial resistance was evaluated and isolates were submitted to clonality by PFGE and MLST. PFG...

Comparative genetic characterization of third-generation cephalosporin-resistant Escherichia coli isolated from a layer operation system in Korea.

The layer operation system has a pyramidal structure in which the grandparent stock (GPS) is at the top, followed by the parent stock (PS), and commercial chickens (CC) that produce eggs at the bottom of the pyramid. Unfortunately, this vertical integration of the layer industry allows the transfer of Escherichia coli(E. coli) to the next step. The objective of this study was to genetically characterize and investigate the prevalence of third-generation cephalosporin-resistant E. coli at all levels of the l...

Age-Associated Distribution of Antimicrobial-Resistant Salmonella enterica and Escherichia coli Isolated from Dairy Herds in Pennsylvania, 2013-2015.

Antimicrobial resistance has become a major global public health concern, and agricultural operations are often implicated as a source of resistant bacteria. This study characterized the prevalence of antimicrobial-resistant Salmonella enterica and Escherichia coli from a total of 443 manure composite samples from preweaned calves, postweaned calves, dry cows, and lactating cows from 80 dairy operations in Pennsylvania. A total of 1095 S. enterica and 2370 E. coli isolates were screened and tested for resis...

Sensitivity and resistance to antimicrobial agents ESBL-producing and not producing ESBL strains of E. coli in patients with urinary tract infection.

As a result of the conducted researches it is shown that 44.1% of urinary tract infections (UTIS) caused by E. coli are accounted for by producers of beta-lactamase of the extended spectrum of action (ESBL). Associated resistance to fluoroquinolones and co-trimoxazole was found in 93.3% of BLRS-producing E. coli strains. All studied strains regardless of ESBL production were sensitive to imipenem, the majority showed sensitivity to ertapenem, gentamicin and resistance to doxycycline. Not producing ESBL stra...

Measurement and prediction of antimicrobial resistance in bloodstream infections by ESKAPE and Escherichia coli pathogens.

The aim of the study was to evaluate a cumulative antimicrobial resistance index (ARI) as a possible key outcome measure of antimicrobial stewardship (AS) programs and as a tool to predict antimicrobial resistance (AMR) trend.

Genetic diversity, antimicrobial resistance and virulence profile of Salmonella isolated from the peanut supply chain.

Thirty-Eight Salmonella isolates recovered from different stages of the peanut supply chain in three Brazilian States (São Paulo, Minas Gerais and Bahia) were subtyped by pulsed-field gel electrophoresis (PFGE) and characterized by phenotypic and genotypic tests for antimicrobial resistance and virulence genes. The isolates were distributed into seven PFGE pulsotypes. All the isolates were resistant to sulfonamide. However, only one isolate from a production site in Minas Gerais had resistance to two types...

Prevalence of colistin resistance and mcr-1/mcr-2 genes in ESBL/AmpC-producing E. coli isolated from chickens in Canada (Quebec), Senegal and Vietnam.

This study investigated the prevalence of E. coli colistin resistance and mcr-1 and mcr-2 genes among ESBL/AmpC-producer E. coli isolates recovered from chicken feces in Canada (Quebec), Senegal and Vietnam, and evaluated the susceptibility pattern of the colistin-resistant E. coli isolates to other clinically relevant antimicrobials.

Colonic adenoma-associated Escherichia coli express specific phenotypes.

Specific Escherichia coli strains have been associated to colorectal cancer, while no data are available on genotypic and phenotypic features of E. coli colonizing premalignant adenomatous polyps and their pathogenic potential. This study was aimed at characterizing isolates collected from polyps and adjacent tissue in comparison with those from normal mucosa. From colonoscopy biopsies, 1500 E. coli isolates were retrieved and genotyped; 272 were characterized for phylogroup and major phenotypic traits (i.e...

Diversity of Extended-Spectrum Cephalosporin-Resistant Escherichia coli in Feces from Calves and Cows on Pennsylvania Dairy Farms.

The global incidence of human infections associated with extended-spectrum β-lactamase (ESBL)-producing Escherichia coli is increasing. Dairy animals are reservoirs of ESBL-producing E. coli, especially, third-generation cephalosporin (3GC)-resistant strains. To further understand the diversity of 3GC-resistant E. coli across animals of different age groups (e.g., pre- and postweaned calves, lactating cows, and dry cows) and farms, we used pulsed-field gel electrophoresis (PFGE) to characterize 70 fecal is...

Antibiotic resistance pattern and virulence genesin avian pathogenic Escherichia coli (APEC) from different breeding systems.

Colibacillosis is the most frequent bacterial disease in avian species and antimicrobials are the main weapon to reduce incidence and mortality associated to it. However, indiscriminate use of antibiotics may lead to therapy failure and economic losses for the breeder. The aims of this study were to, determine the antibiotic resistance of Escherichia coli isolates, evaluate the correlation between E. coli isolation and systems of breeding included in this study, and identify the avian pathogenic E.coli (APE...

Diversity and antibiotic resistance among Escherichia coli populations in hospital and community wastewater compared to wastewater at the receiving urban treatment plant.

Bacterial diversity and antimicrobial resistance patterns among the indicator organism Escherichia coli were monitored in wastewater samples collected over one year from a hospital (HW), a community (CW) and the receiving urban (UW) wastewater treatment plant (WWTP). We compared levels of antibiotic resistance in the different types of wastewater, and identified whether resistant strains were endemic in the wastewater system. If so, implementation of local treatment at certain resistance hotspots (e.g. hosp...

Core genome sequence analysis to characterize Salmonella enterica serovar Rissen ST469 from a swine production chain.

Salmonella enterica subsp. enterica serotype Rissen is the predominant serotype found in Thai pork production and can be transmitted to humans through contamination of the food chain. This study was conducted to investigate the genetic relationships between serovar Rissen isolates from all levels of the pork production chain and evaluate the ability of the in silico antimicrobial resistance (AMR) genotypes to predict the phenotype of serovar Rissen. A total of 38 serovar Rissen isolates were tested against ...

The emergence of metronidazole and vancomycin reduced-susceptibility in Clostridium difficile isolates in Iran.

Clostridium difficile is the main causative agent of antibiotic-associated diarrhoea (AAD) and pseudomembranous colitis (PMC). The accumulation of antimicrobial resistance in C. difficile strains can drive Clostridium difficile infection (CDI) epidemiology. This study was undertaken in order to evaluate the antimicrobial resistance patterns of toxigenic C. difficile isolates cultured from diarrhoeal stool samples of hospitalised patients with suspected CDI in three tertiary care hospitals in Tehran, Iran.


Advertisement
Quick Search
Advertisement
Advertisement