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PubMed Journals Articles About "Shear Rheological Properties Acid Hydrolyzed Insoluble Proteins From" RSS

19:11 EDT 20th June 2019 | BioPortfolio

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Showing "Shear rheological properties acid hydrolyzed insoluble proteins from" PubMed Articles 1–25 of 26,000+

Shear rheological properties of acid hydrolyzed insoluble proteins from Chlorella protothecoides at the oil-water interface.

Microalgae are promising protein sources due to their overall high protein content. The low aqueous-solubility of microalgae proteins, however, limits their application in food, pharmaceutical or personal care systems, unless solubility is enhanced by e.g. hydrolysis. In this study, we examined the interfacial rheological properties at the oil-water interface of insoluble microalgae protein-rich fraction from Chlorella protothecoides and their hydrolysates prepared by hydrolysis in hydrochloric acid at 65...


Analysis of the relationship of extracellular polymeric substances to the dewaterability and rheological properties of sludge treated by acidification and anaerobic mesophilic digestion.

The initial pH value of sludge affects sludge anaerobic digestion and dewatering performance. In order to determine the suitable pH value and decrease the sludge digestion time, in this study, the effect and the mechanism of combined acidification and anaerobic mesophilic digestion (acid-AMD) on sludge dewaterability were investigated. The changes and relationships among the extracellular polymeric substances (EPS), physicochemical properties, and rheological behavior of the treated sludge were analyzed. Th...

Preparation of different polymorphs of cellulose from different acid hydrolysis medium.

In this study, medical cotton was subjected to acid hydrolysis in sulfuric, hydrochloric and phosphoric acid medium to prepare cellulose nanocrystals (CNC) with different morphologies and polymorphism. Morphology of the prepared CNC samples revealed fiber shaped morphology for sulfuric and hydrochloric acid hydrolyzed samples, whereas, spherical shaped for phosphoric acid hydrolyzed samples. The size of the spherical shaped CNC decreased with the increase of hydrolysis time, from 853 nm for 12 h to 187...


Rheological Properties and Age-Related Changes of the Human Vitreous Humor.

The vitreous humor is a fragile, transparent hydrogel situated between the lens and the retina, occupying 80% of the eye's volume. Due to its viscoelastic behavior, the vitreous serves as a mechanical damper for the eye, absorbing impacts, and protecting the lens and retina. The vitreous liquefies with age, which compromises its function as a shock absorber and causes complications including retinal detachment, macular holes, and vitreous hemorrhage. Studies on the viscoelastic properties of the vitreous ha...

Paired versus two-group experimental design for rheological studies of vocal fold tissues.

Vibratory function of the vocal folds is largely determined by the rheological properties or viscoelastic shear properties of the vocal fold lamina propria. To date, investigation of the sample size estimation and statistical experimental design for vocal fold rheological studies is nonexistent. The current work provides the closed-form sample size formulas for two major study designs (i.e. paired and two-group designs) in vocal fold research. Our results demonstrated that the paired design could greatly in...

Rheological properties of Prunus persica exudate: Potential effects of proteins and polyphenols.

This study was to investigate the rheological properties of graded Prunus persica exudates (peach gums). Graded peach gums were extracted by hot water and precipitated by ethanol to obtain white peach gum polysaccharide (WPGP) and yellow peach gum polysaccharide (YPGP). Chemical analysis showed that YPGP had 0.11% proteins and 0.23% polyphenols, which were not detected in WPGP. Molecular weight of WPGP and YPGP were determined as 6.09 × 10 kDa and 5.82 × 10 kDa, respectively, by using HPGPC. M...

Peptidomic analysis of hydrolyzed oat bran proteins, and their in vitro antioxidant and metal chelating properties.

Peptide profiles of hydrolyzed oat proteins and the susceptibility of their polypeptides to proteolytic cleavages were determined using peptidomic analysis. In addition, antioxidant activities were also measured. Proteins isolates were first extracted with carbohydrases, Viscozyme or Cellulase and then hydrolyzed with proteases (Alcalase, Papain, Protamex, Flavourzyme). Amongst the eight hydrolysates, Viscozyme-proteins hydrolyzed with Papain showed the highest ability to quench ABTS radicals (866.9 ± ...

Rheological characterization of thermal hydrolysed waste activated sludge.

Rheological properties are important in the design and operation of sludge-handling process. Despite this, the rheology of sludge in thermal hydrolysis processes (TH) is not well described. In-situ measurements were performed to characterize the flow behaviour of various concentrations (7-13 wt%) of waste activated sludge (WAS) at TH conditions. Equations were presented for predicting in-situ rheological parameters (high-shear viscosity, η, consistency index, k, and yield stress, σ) under various treatm...

Structural characterization and rheological properties of a pectin with anti-constipation activity from the roots of Arctium lappa L.

A new pectin (ALP-2) was extracted from the roots of Arctium lappa L. with the molecular weight of 1.84 × 10 Da. ALP-2 was composed of rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and arabinose. Results of NMR revealed that the dominant linkage types of ALP-2 were →4-α-GalpA-6-OMe-(1→, →2-α-Rha-(1→, →5-α-Araf-(1→ and →3,6-β-Galp-(1→. The ELISA results indicated ALP-2 was a typical pectin with HG chain and RG-I chain. The rheological experiments showed tha...

Impact of soy proteins, hydrolysates and monoglycerides at the oil/water interface in emulsions on interfacial properties and emulsion stability.

The effect of the adsorption behavior of soy proteins hydrolysates and monoglycerides on the properties of the film at oil-water interfaces in emulsions and emulsion stability was investigated. Surface tension, adsorbed protein composition and interfacial rheological properties were measured to gain insights into the relationships between molecular composition and properties. The interfacial film formed by 7S (β-conglycinin), 11S (Glycinin) and native soy protein isolate (NSPI) exhibited high elasticity du...

Sunflower oil cake-derived cellulose nanocrystals: Extraction, physico-chemical characteristics and potential application.

In this work, sunflower oil cake (SOC) was identified as bio-sourced material for cellulose nanocrystals (CNC) production using chemical treatments followed by sulfuric acid hydrolysis. The hydrolysis was performed at 64% acid concentration, a temperature of 50 °C and at two different hydrolysis times, 15 min (CNC) and 30 min (CNC). It was found that CNC exhibited a diameter of 9 ± 3 nm and 5 ± 2 nm, a length of 354 ± 101 nm and 329 ± 98 nm, a crystallinity of 75% and 87%,...

Influence of the welan gum biopolymer concentration on the rheological properties, droplet size distribution and physical stability of thyme oil/W emulsions.

The objective of this work is to obtain a stable and natural antimicrobial delivery system. Thus, the effect of the addition of a natural polysaccharide such as welan gum on the linear viscoelastic properties, flow behaviour, droplet size distribution and physical stability of thyme oil/W emulsions formulated with a wheat-derived surfactant was studied. All emulsions obtained show submicron diameters regardless of the concentration of welan. Emulsion without gum shows Newtonian behaviour under steady shear....

Effective tool for assessment of the quality of barrier creams - relationships between rheological, textural and sensory properties.

The efficiency of barier creams (BC) in the prevention of occupational skin diseases is closely related to their mechanical, rheological but also sensory properties. The measurement procedure we elaborated, which simulates the spreadability conditions on the skin and evaluation whether the structure reconstruction occurs (hysteresis loop test, shear rate dependence of normal force), allows the assessment of the effectivness of the BC in terms of mechanical resistance and its adhesion to the skin surface. In...

Evaluation of modified hyaluronic acid in terms of rheology, enzymatic degradation and mucoadhesion.

This study aimed to investigate the properties of modified hyaluronic acid in terms of rheological properties, enzymatic degradation and mucoadhesiveness.

3-D printing of chitosan-calcium phosphate inks: rheology, interactions and characterization.

Bone substitute fabrication is of interest to meet the worldwide incidence of bone disorders. Physical chitosan hydrogels with intertwined apatite particles were chosen to meet the bio-physical and mechanical properties required by a potential bone substitute. A set up for 3-D printing by robocasting was found adequate to fabricate scaffolds. Inks consisted of suspensions of calcium phosphate particles in chitosan acidic aqueous solution. The inks are shear-thinning and consist of a suspension of dispersed ...

Japanese grape (Hovenia dulcis) polysaccharides: New insight into extraction, characterization, rheological properties, and bioactivities.

In this study, accelerated solvent extraction (ASE) technology was applied to extract Hovenia dulcis polysaccharides (HDPs). The polysaccharide yields, physicochemical properties, rheological properties, antioxidant activities, and hypoglycemic effects were compared between ASE-HDPs and hot water extracted polysaccharides (HWE-HDPs). The maximum ASE-HDPs yield was obtained using a response surface method (RSM) with optimized ASE conditions comprising 2 cycles at 130 °C for 23 min. The maximum ASE-HDP...

Preparation of in situ hydrogels loaded with azelaic acid nanocrystals and their dermal application performance study.

Azelaic acid (AZA) is a dicarboxylic acid that is topically used in the treatment of acne and rosacea since it possesses antibacterial and keratolytic activity. The primary objective of this study was to develop an AZA nanocrystal suspension. It is expected that improved solubility and dissolution rate will result in advanced biopharmaceutical properties, primarily the dermal bioavailability. Furthermore, a topical nanocrystal AZA-loaded hydrogels composed of Pluronic® F127 and hyaluronic acid mixture that...

Highlighting protein fining residues in a model red wine.

Many studies have dealt with fining treatments, focusing on their impact on phenolic precipitation or sensory properties of the treated wines. Previous articles suggested the presence of soluble complexes with tannins and fining proteins, and probably wine polysaccharides too. However, no study has quantified these possible protein residues in wine. The analyses performed on a model red wine to highlight the residual fining proteins were the measurement of viscosity, the quantification of amino acid/protein...

Probing shear-induced rearrangements in Fourier space. II. Differential dynamic microscopy.

We discuss in two companion papers how Fourier-space measurements may be coupled to rheological tests in order to elucidate the relationship between mechanical properties and microscopic dynamics in soft matter. In this second companion paper, we focus on Differential Dynamic Microscopy (DDM) under shear. We highlight the analogies and the differences with dynamic light scattering coupled to rheology, providing a theoretical approach and practical guidelines to separate the contributions to DDM arising from...

Delivery of progenitor cells with injectable shear-thinning hydrogel maintains geometry and normalizes strain to stabilize cardiac function after ischemia.

The ventricle undergoes adverse remodeling after myocardial infarction, resulting in abnormal biomechanics and decreased function. We hypothesize that tissue-engineered therapy could minimize postischemic remodeling through mechanical stress reduction and retention of tensile myocardial properties due to improved endothelial progenitor cell retention and intrinsic biomechanical properties of the hyaluronic acid shear-thinning gel.

Rheological properties of fresh and reconstituted milk protein concentrates under standard and processing conditions.

As the processability of fresh and reconstituted milk protein concentrates crucially depends on their rheological properties, a considerable amount of studies focuses on this topic. By means of a direct comparison, we are the first to clearly show that distinct rheological differences can exist between fresh and reconstituted milk protein concentrates under standard and processing conditions. We show that reconstituted milk protein concentrates made from commercial milk protein powders exhibit higher viscos...

Effect of ethanol content on rheology of film-forming solutions and properties of zein/chitosan film.

The paper focused on the role of ethanol on rheological properties of zein/chitosan film-forming solutions, thermo-mechanical and structural characterization of films. The steady rheological results indicated that the film-forming solutions showed non-Newtonian behavior, and ZC-3 solution (zein treated with 90% ethanol and chitosan dissolved with acetic acid) had higher flow index (0.882 ± 0.003). Based on dynamic rheological analysis, ZC-3 solution showed the highest G' value and the crossover point a...

Impact of mechanical history on sludge rheological properties: Role of the organic content.

The rheological behavior is known to be of great interest in sludge treatment, but the material complexity induces strong measurement difficulties. The literature highlights two characteristics deeply impacting sludge rheological properties and their characterization: Thixotropic behavior and organic matter (Seyssiecq et al., 2003; Eshtiaghi et al., 2013), both being related to each other. Indeed, by looking at how thixotropy affects elastic modulus and yield stress with different mechanical histories at ...

Microbial transglutaminase induced controlled crosslinking of gelatin methacryloyl to tailor rheological properties for 3D printing.

Gelatin methacryloyl (GelMA) is a versatile biomaterial that has been shown to possess many advantages such as good biocompatibility, support for cell growth, tunable mechanical properties, photocurable capability, and low material cost. Due to these superior properties, much research has been carried out to develop GelMA as a bioink for bioprinting. However, there are still many challenges, and one major challenge is the control of its rheological properties to yield good printability. Herein, this study p...

Rheological and Microstructural Characteristics of Canola Protein Isolate-Chitosan Complex Coacervates.

Wastage of byproducts such as canola meal is a pressing environmental concern, and canola protein isolate (CPI)-chitosan (Ch) coacervates have a good potential to utilize and convert the wastes into a high value added product. Yet so far, there is very limited rheological and microstructural information to assist in proper utilization of CPI -Ch complex coacervates. The rheological and microstructural properties of the complex coacervates formed from CPI and chitosan Ch at various CPI-to-Ch mixing ratios (1...


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