Advertisement

Topics

PubMed Journals Articles About "Structural Insights Into NanoRNA Degradation Human Rexo2" RSS

07:27 EDT 26th May 2019 | BioPortfolio

Structural Insights Into NanoRNA Degradation Human Rexo2 PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest Structural Insights Into NanoRNA Degradation Human Rexo2 articles that have been published worldwide.

More Information about "Structural Insights Into NanoRNA Degradation Human Rexo2" on BioPortfolio

We have published hundreds of Structural Insights Into NanoRNA Degradation Human Rexo2 news stories on BioPortfolio along with dozens of Structural Insights Into NanoRNA Degradation Human Rexo2 Clinical Trials and PubMed Articles about Structural Insights Into NanoRNA Degradation Human Rexo2 for you to read. In addition to the medical data, news and clinical trials, BioPortfolio also has a large collection of Structural Insights Into NanoRNA Degradation Human Rexo2 Companies in our database. You can also find out about relevant Structural Insights Into NanoRNA Degradation Human Rexo2 Drugs and Medications on this site too.

Showing "Structural insights into nanoRNA degradation human Rexo2" PubMed Articles 1–25 of 28,000+

Structural insights into nanoRNA degradation by human Rexo2.

Human RNA exoribonuclease 2 (Rexo2) is an evolutionarily conserved 3'-to-5' DEDDh-family exonuclease located primarily in mitochondria. Rexo2 degrades small RNA oligonucleotides of less than five nucleotides (nanoRNA) in a way similar to E. coli Oligoribonuclease (ORN), suggesting that it plays a role in RNA turnover in mitochondria. However, how Rexo2 preferentially binds and degrades nanoRNA remains elusive. Here, we show that Rexo2 binds small RNA and DNA oligonucleotides with the highest affinity, and i...


Structural states of Hdm2 and HdmX: X-ray elucidation of adaptations and binding interactions for different chemical compound classes.

Hdm2 (human MDM2) counteracts p53 function by direct binding to p53 and by ubiquitin-dependent p53 protein degradation. Activation of p53 by inhibitors of the p53-Hdm2 interaction is being pursued as a therapeutic strategy in p53 wild-type cancers. In addition, HdmX (human MDMX, human MDM4) was also identified as an important therapeutic target to efficiently reactivate p53, and it is likely that dual inhibition of Hdm2 and HdmX is beneficial. Here, we report four new X-ray structures for Hdm2 and five new ...

A study on structural characterization of degradation products of cangrelor using LC/QTOF/MS/MS and NMR.

A complete degradation study was performed on cangrelor drug substance as per the ICH guidelines. The study reveals that a total of six degradation products (DP-1 to DP-6) were found and out of these, three unknown degradation products (DP-1, DP-5 and DP-6) were not reported in the literature. Based on the degradation study, the drug substance cangrelor was found to be sensitive towards acidic, basic and oxidative conditions. Besides, it was stable under thermal and photolytic stress conditions. The degrada...


Protein Degradation and the Pathologic Basis of Disease.

The abundance of any protein is determined by the balance of protein synthesis and protein degradation. Regulated protein degradation has emerged as a powerful means of precisely controlling individual protein abundance within cells, and is largely mediated by the ubiquitin-proteasome system (UPS). By controlling the levels of key regulatory proteins, the UPS contributes to nearly every aspect of cellular function. The UPS also functions in protein quality control, rapidly identifying and destroying misfold...

Structural and biochemical analysis of a NOT1 MIF4G-like domain of the CCR4-NOT complex.

The CCR4-NOT complex plays a central role in the regulation of gene expression and degradation of messenger RNAs. The multisubunit complex assembles on the NOT1 protein, which acts as a 'scaffold' and is highly conserved in eukaryotes. NOT1 consists of a series of helical domains that serve as docking sites for other CCR4-NOT subunits. We describe a crystal structure of a connector domain of NOT1 from the thermophilic fungus Chaetomium thermophilum (Ct). Comparative structural analysis indicates that this d...

Identification and structural characterization of hydrolytic degradation products of alvimopan by LC/QTOF/MS/MS and NMR studies.

Alvimopan (ALV), a peripherally acting mu-opioid receptor (PAM-OR) antagonist used for the treatment of postoperative ileus, was examined for its degradation behaviour under different stress conditions. A total of five degradation products (DP1-DP5) were formed and identified using high-performance liquid chromatography (HPLC) method. Primarily, complete fragmentation pathways of the protonated drug and its degradation products (DP1-DP5) were elucidated by using liquid chromatography-mass spectrometry (LC/M...

Identification of competitive inhibitors of the human taurine transporter TauT in a human kidney cell line.

The osmolyte and antioxidant taurine plays an important role in regulation of cellular volume, oxidative status and Ca-homeostasis. Taurine uptake in human cells is regulated by the Na- and Cl-dependent taurine transporter TauT. In order to gain deeper structural insights about the substrate binding pocket of TauT, a HEK293 cell line producing a GFP-TauT fusion protein was generated.

Endocytosis-dependent lysosomal degradation of Src induced by protease-activated receptor 1.

Src plays a critical role in regulating cellular responses induced by protease-activated receptor 1 (PAR1). Here, we found that PAR1 activation induces lysosomal degradation of Src. Src is associated and trafficked together with activated PAR1 to early endosomes and then sorted to lysosomes for degradation. Blocking agonist-induced endocytosis of PAR1 by inhibition of dynamin activity suppresses PAR1-induced degradation of Src. However, Src activity is not required for agonist-induced PAR1 internalization; ...

Comparative study of keratin extraction from human hair.

Keratin has been attracting interest due to its stability against enzymatic degradation thereby allowing more predictable degradation profile for tissue regeneration. While the efficacy of keratin has been demonstrated in different tissue models, there has been no systematic study to investigate and compare the different routes of keratin extraction from human hair. Here, we compared the four commonly used extraction methods and highlight the both physical and chemical differences in the extracted keratin. ...

Structural Basis for Neutralization and Protection by a Zika Virus-Specific Human Antibody.

We previously reported a human monoclonal antibody, ZK2B10, capable of protection against Zika virus (ZIKV) infection and microcephaly in developing mouse embryos. Here, we report the structural features and mechanism of action of ZK2B10. The crystal structure at a resolution of 2.32 Å revealed that the epitope is located on the lateral ridge of DIII of the envelope glycoprotein. Cryo-EM structure with mature ZIKV showed that the antibody binds to DIIIs around the icosahedral 2-fold, 3-fold, and 5-fold a...

Structural basis for the bypass of the major oxaliplatin-DNA adducts by human DNA polymerase η.

Oxaliplatin, together with cisplatin, is among the most important drugs used in cancer chemotherapy. Oxaliplatin, which contains a bulky diaminocyclohexane (DACH) moiety, kills cancer cells mainly by producing (DACH)Pt-GpG intrastrand cross-links that impede transcription. The Pt-GpG tolerance by translesion DNA synthesis (TLS) polymerases contributes to the resistance of tumors to platinum-based chemotherapy. In particular, human DNA polymerase η (Polη) readily bypasses Pt-GpG adducts. While many structu...

Structural insights of stereospecific inhibition of human acetylcholinesterase by VX and subsequent reactivation by HI-6.

Over 50 years ago, the toxicity of irreversible organophosphate inhibitors targeting human acetylcholinesterase (hAChE) were observed to be stereospecific. The therapeutic reversal of hAChE inhibition by reactivators has also been shown to depend on the stereochemistry of the inhibitor. To gain clarity on the mechanism of stereospecific inhibition, the X-ray crystallographic structures hAChE inhibited by a racemic mixture of VX (P) and its enantiomers were obtained. Beyond identifying hAChE structural featu...

Protective effects of alogliptin against TNF-α-induced degradation of extracellular matrix in human chondrocytes.

Osteoarthritis (OA) is a common debilitating disease most prevalent among the elderly population worldwide. Excessive degradation of the articular extracellular matrix is a pivotal event in the development of OA. Preventative treatments against the destruction of type II collagen and aggrecan, the two main components of the articular extracellular matrix, may serve as a novel therapy against the progression of OA. In the current study, we investigated whether the DPP-4 inhibitor alogliptin could prevent deg...

Structural insights into substrate selectivity, catalytic mechanism and redox regulation of rice photosystem II core phosphatase.

Photosystem II (PSII) core phosphatase (PBCP) selectively dephosphorylates PSII core proteins including D1, D2, CP43 and PsbH. The function of PBCP is required for efficient degradation of D1 protein in the repair cycle of PSII, a supramolecular machinery highly susceptible to photodamage during oxygenic photosynthesis. Here we present structural and functional studies of PBCP from Oryza sativa (OsPBCP). In a symmetrical homodimer of OsPBCP, each monomer contains a PP2C-type phosphatase core domain, a large...

Enhanced surface Fenton degradation of BPA in soil with a high pH.

Although the heterogeneous Fenton process of iron-bearing minerals has been widely studied due to its potential use for the removal of organic pollutants, the transformation mediated by Fe species in soil particles remains poorly understood. Here, we compared the removal of bisphenol A (BPA) from soil using a Fenton system at low and high pH values. At low pH value, the BPA removal rate decreased with increasing pH value; this result was consistent with the amount of soluble Fe(II) and surface-bound Fe(II) ...

Synaptic organizer: Slitrks and type IIa receptor protein tyrosine phosphatasess.

Slit-like and Trk-like (Slitrk) family members are leucine-rich repeat (LRR)-containing neuronal transmembrane proteins. Slitrks have been highlighted as key synapse organizers at neuronal synapses through interactions with specific members of the presynaptic type IIa receptor protein tyrosine phosphatase (RPTP) family. Recent structural studies on type IIa RPTP/Slitrk1 complexes have unveiled molecular insights into their binding selectivity and have established the role of higher-order receptor clustering...

Structures of the human pre-catalytic spliceosome and its precursor spliceosome.

The pre-catalytic spliceosome (B complex) is preceded by its precursor spliceosome (pre-B complex) and followed by the activated spliceosome (B complex). The pre-B-to-B and B-to-B transitions are driven by the ATPase/helicases Prp28 and Brr2, respectively. In this study, we report the cryo-electron microscopy structures of the human pre-B complex and the human B complex at an average resolution of 5.7 and 3.8 Å, respectively. In the pre-B complex, U1 and U2 small nuclear ribonucleoproteins (snRNPs) assoc...

E6 proteins from high-risk HPV, low-risk HPV, and animal papillomaviruses activate the Wnt/β-catenin pathway through E6AP-dependent degradation of NHERF1.

High-risk human papillomavirus (HPV) E6 proteins associate with the cellular ubiquitin ligase E6-Associated Protein (E6AP), and then recruit both p53 and certain cellular PDZ proteins for ubiquitination and degradation by the proteasome. Low-risk HPV E6 proteins also associate with E6AP, yet fail to recruit p53 or PDZ proteins; their E6AP-dependent targets have so far been uncharacterized. We found a cellular PDZ protein called Na+/H+ Exchanger Regulatory Factor 1 (NHERF1) is targeted for degradation by bot...

Enrichment of Relevant Oxidative Degradation Products in Pharmaceuticals with Targeted Chemoselective Oxidation.

The ability to produce and isolate relatively pure amounts of relevant degradation products is key to several aspects of drug product development: (a) aid in the unambiguous structural identification of such degradation products, fulfilling regulatory requirements to develop safe formulations (ICH Q3B and M7); (b) pursue as appropriate safety evaluations with such material, such as chronic toxicology or Ames testing; (c) for a specified degradation product in a late-stage regulatory filing, utilize pure and...

UBC9 regulates cardiac sodium channel Na1.5 ubiquitination, degradation and sodium current density.

Voltage-gated sodium channel Na1.5 is critical for generation and conduction of cardiac action potentials. Mutations and expression level changes of Na1.5 are associated with cardiac arrhythmias and sudden death. The ubiquitin (Ub) conjugation machinery utilizes three enzyme activities, E1, E2, and E3, to regulate protein degradation. Previous studies from us and others showed that Nedd4-2 acts as an E3 ubiquitin-protein ligase involved in ubiquitination and degradation of Na1.5, however, more key regulator...

Degradation studies of Ibrutinib under stress conditions: Characterisation and structural elucidation of novel degradants.

The aim of the research work is to study the degradation behaviour of Ibrutinib (IBN) which is performed under different stress conditions according to International Conference on Harmonization guidelines (ICH). The study included monitoring degradation of the Ibrutinib drug under acidic, base, oxidation, thermal and photolytic conditions followed by isolation and characterisation of degradation products (DP) by Liquid Chromatography Mass Spectrometry (LCMS), High resolution Mass Spectrometry (HR-MS/MS) and...

Photocatalytic degradation of trihalomethanes and haloacetonitriles on graphitic carbon nitride under visible light irradiation.

Trihalomethanes (THMs) and haloacetonitriles (HANs), most common disinfection by-products in drinking water, pose adverse environmental impacts and potential risks to human health. There is a pressing need to develop innovative, economically feasible, and environmentally benign processes to control these persistent contaminants. In this paper, visible-light-responsive graphitic carbon nitride (g-CN) samples were synthesized to degrade the THMs and HANs and the photocatalytic degradation mechanism was explor...

Roles of reactive oxygen species (ROS) in the photocatalytic degradation of pentachlorophenol and its main toxic intermediates by TiO/UV.

Pentachlorophenol (PCP) caused water quality problems owe to its past widespread application and stability, harmful to human health. Photocatalysis, which was mainly involved in the reactive oxygen species (ROS) reaction, has large potential as water treatment process. However, the roles of ROS on the degradation process of PCP are not yet clearly defined. The main objectives of this work were to investigate the roles of ROS involved in the whole degradation of PCP and main toxic intermediates and elucidate...

Progress in human picornavirus research: New findings from the AIROPico consortium.

Several research groups in Europe are active on different aspects of human picornavirus research. The AIROPico (Academia-Industry R&D Opportunities for Picornaviruses) consortium combined the disciplines of pathogenesis, diagnostics and therapy development in order to fill the gaps in our understanding of how picornaviruses cause human disease and how to combat them. AIROPico was the first EU consortium dedicated to human picornavirus research and development, and has largely accelerated and improved R&D on...

Ocular Component Development during Infancy and Early Childhood.

The study fills an important gap by providing a longitudinal description of development of the major structural and optical components of the human eye from 3 months to nearly 7 years of age. Normative development data may provide insights into mechanisms for emmetropization and guidance on intraocular lens power calculation.


Advertisement
Quick Search
Advertisement
Advertisement