PubMed Journals Articles About "Tellurate Enters Escherichia Coli Cells SulT Type Sulfate" RSS

15:36 EST 22nd January 2019 | BioPortfolio

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Showing "Tellurate enters Escherichia coli cells SulT type sulfate" PubMed Articles 1–25 of 34,000+

Complete and assembled genome sequence of an NDM-5 and CTX-M-15 producing Escherichia coli sequence type 617 isolated from wastewater in Switzerland.

Carbapenem-resistant Escherichia coli have emerged worldwide and represent a major challenge to effective health care management. Here, we report the genome sequence of an NDM-5 and CTX-M-15 producing E. coli belonging to sequence type 617, isolated from wastewater treatment plant effluent in Switzerland.

Activity of ceftolozane-tazobactam against Escherichia coli isolates from U.S. veterans (2011) in relation to co-resistance and sequence type 131 (ST131) H30 and H30Rx status.

Escherichia coli sequence type 131 (ST131), with its multidrug-resistance-associated H30R1 and H30Rx clonal subsets, causes most antimicrobial-resistant E. coli infections in the U.S., especially among veterans. The activity of ceftolozane-tazobactam (C/T), a new beta-lactamase inhibitor agent, against ST131 strains, and E. coli isolates from veterans, is undefined.

High level in vivo mucin-type glycosylation in Escherichia coli.

Increasing efforts have been made to assess the potential of Escherichia coli strains for the production of complex recombinant proteins. Since a considerable part of therapeutic proteins are glycoproteins, the lack of the post-translational attachment of sugar moieties in standard E. coli expression strains represents a major caveat, thus limiting the use of E. coli based cell factories. The establishment of an E. coli expression system capable of protein glycosylation could potentially facilitate the prod...

Surface Display of Bacterial Laccase CotA on Escherichia coli Cells and its Application in Industrial Dye Decolorization.

Laccase CotA from Bacillus subtilis 168 was successfully displayed on the membrane of Escherichia coli cells using poly-γ-glutamate synthetase A protein (PgsA) from B. subtilis as an anchoring matrix. Further analyses demonstrated that the fusion protein PgsA/CotA efficiently translocates to the cell surface of E. coli with an enzymatic activity of 65 U/10 cells. Surface-displayed CotA was shown to possess improved enzymatic properties compared with those of the wild-type CotA, including higher thermal st...

Virulence factor-dependent basolateral invasion of choroid plexus epithelial cells by pathogenic Escherichia coli in vitro.

Escherichia coli (E. coli) is the most common Gram-negative causative agent of neonatal meningitis and E. coli meningitis is associated with a high morbidity and mortality. Previous research has been carried out with regards to the blood-brain barrier and thereby unveiled an assortment of virulence factors involved in E. coli meningitis. Little, however, is known about the role of the blood-cerebrospinal fluid (CSF) barrier (BCSFB), in spite of several studies suggesting that the choroid plexus (CP) is a po...

The different roles of hcp and hcp of the type VI secretion system in Escherichia coli strain CE129.

Type VI secretion system (T6SS) is a secretory system found in Gram-negative bacteria. One of the main structures for T6SS is Hcp (hemolysin co-regulation protein) pipeline. To investigate the role of Hcp major sub-unit genes hcp and hcp, we deleted hcp and hcp genes for constructing the in-frame gene deletion mutants. The properties of biofilm formation and the adhesion to chicken embryo fibroblasts cells (DF1 cells) were reduced in the hcp mutant. The knockout of hcp and hcp genes reduced the ability of t...

An electrochemical immunobiosensor for ultrasensitive detection of Escherichia coli O157:H7 using CdS quantum dots-encapsulated metal-organic frameworks as signal-amplifying tags.

We report here cadmium sulfide quantum dots (CdS QDs)-encapsulated metal-organic frameworks as signal-amplifying tags for ultrasensitive electrochemical detection of Escherichia coli O157:H7 (E. coli O157:H7). CdS QDs were encapsulated in zeolitic imidazolate framework-8 (ZIF-8) to form CdS@ZIF-8 muti-core-shell particles by in situ growth of ZIF-8 in the presence of CdS QDs. To specifically recognize E. coli O157:H7 cells, CdS@ZIF-8 particles were coated with polyethyleneimine to introduce amino groups on ...

Detection of viable Escherichia coli in environmental water using combined propidium monoazide staining and quantitative PCR.

The objectives of this study were to specifically detect viable Escherichia coli in environmental waters by targeting the ycjM gene in a propidium monoazide (PMA)-qPCR assay. PMA is a viability dye that can inhibit the amplification of DNA from dead cells, thus allowing for the detection and quantification of only viable cells. The ycjM primers were used to target E. coli that directly originated from the feces of warm blooded animals, and avoid false positive detection caused by "naturalized" E. coli tha...

Absence of RstA results in delayed initiation of DNA replication in Escherichia coli.

RstB/RstA is an uncharacterized Escherichia coli two-component system, the regulatory effects of which on the E. coli cell cycle remain unclear. We found that the doubling time and average number of replication origins per cell in an ΔrstB mutant were the same as the wild-type, and the average number of replication origins in an ΔrstA mutant was 18.2% lower than in wild-type cells. The doubling times were 34 min, 35 min, and 40 min for the wild-type, ΔrstB, and ΔrstA strains, respectively. Ectopic expre...

Engineered sigma factors increase full-length antibody expression in Escherichia coli.

Escherichia coli (E. coli) is a promising platform for expression of full-length antibodies owing to its several advantages as a production host (fast growth, well characterized genetics, low manufacturing cost), however, low titers from shake flask (typically

Toxicity of sulfide-modified nanoscale zero-valent iron to Escherichia coli in aqueous solutions.

Sulfide-modified nanoscale zero-valent iron (S/nZVI) has been widely studied for groundwater remediation, but the potential environmental risks are poorly understood. This study examined the toxicity of S/nZVI to Escherichia coli in aqueous solutions. The sulfidation could reduce toxicity of nZVI, and S/nZVI exhibited a weaker toxicity at lower Fe/S molar ratio, resulting from the lower Fe content and higher sulfate and iron oxide. The toxicity of S/nZVI was significantly alleviated in the presence of N-Ace...

Genomic features of a highly virulent ceftiofur-resistant CTX-M-8-producing Escherichia coli ST224 causing fatal infection in a domestic cat.

Escherichia coli strains producing extended-spectrum β-lactamases (ESBL), especially of CTX-M type, have been largely described in companion animals; however genomic data are lacking to clarify the clinical impact of ESBL producers in these hosts. The aim of this study was to present genomic features of a highly virulent ceftiofur-resistant CTX-M-8-producing E. coli lineage from a case of pneumonia in cat with fatal outcome.

ST131 fimH22 Escherichia coli isolate with a blaCMY-2/IncI1/ST12 plasmid obtained from a patient with bloodstream infection: highly similar to E. coli isolates of broiler origin.

This study compares the genome of an ST131 CMY-2-producing Escherichia coli isolate from a Danish patient with other ST131 CMY-2-producing E. coli isolates of both human and animal origin.

Recent developments of quinolone-based derivatives and their activities against Escherichia coli.

Escherichia coli (E. coli) is the most common pathogen in both hospital and community settings, and is capable of causing infections that can lead to serious consequences. Quinolones, one of the most common antibiotics in clinical use, are effective weapons to treat E. coli infections. However, the resistance of E. coli to quinolones develops rapidly and spreads widely. Thus, it has become increasingly urgent to enhance the potency of quinolones against both drug-susceptible and drug-resistant E. coli. ...

Crystal structure of Escherichia coli purine nucleoside phosphorylase complexed with acyclovir.

Escherichia coli purine nucleoside phosphorylase (PNP), which catalyzes the reversible phosphorolysis of purine ribonucleosides, belongs to the family I hexameric PNPs. Owing to their key role in the purine salvage pathway, PNPs are attractive targets for drug design against some pathogens. Acyclovir (ACV) is an acyclic derivative of the PNP substrate guanosine and is used as an antiviral drug for the treatment of some human viral infections. The crystalline complex of E. coli PNP with acyclovir was prepare...

Maternal Carriage of Group B Streptococcus and Escherichia coli in a District Hospital in Mozambique.

In low-income countries, data on prevalence and effects of group B Streptococcus (GBS) and Escherichia coli (E. coli) colonization among pregnant women are scarce, but necessary to formulate prevention strategies. We assessed prevalence of GBS and E. coli colonization and factors associated among pregnant women, its effect in newborns and acceptability regarding the utilized sampling methods in a semirural Mozambican hospital.

Mesenchymal stem cells transplantation attenuates brain injury and enhances bacterial clearance in Escherichia coli meningitis in newborn rats.

Neonatal meningitis caused by Escherichia coli results in significant mortality and neurological disabilities, with few effective treatments. Recently, we demonstrated that human umbilical cord blood-derived mesenchymal stem cell (hUCB-MSC) transplantation attenuated E. coli-induced severe pneumonia, primarily by reducing inflammation and enhancing bacterial clearance. This study aimed to determine whether intraventricular transplantation of hUCB-MSCs attenuated the brain injury in E. coli meningitis in new...

Comparative proteomic analysis of Escherichia coli O157:H7 following ohmic and water bath heating by capillary-HPLC-MS/MS.

Escherichia coli O157:H7 is an important food-borne pathogenic microorganism that has been used as a model organism for studying microbial inactivation effects and inactivation mechanism in various sterilization technologies. The objective of this study was to investigate the effects of high voltage short time ohmic- (HVST), low voltage long time ohmic- (LVLT), and water bath- (WB) heating on inactivation and proteome changes of E. coli O157:H7 cells at the same endpoint temperature of 72 °C, and to anal...

Draft genome sequence of colistin-resistant Escherichia coli ST226: A clinical strain harboring a mcr-1 variant.

Escherichia coli isolates carrying mcr-1 are rarely reported in diarrheal patients. We report here the draft genome sequence of colistin-resistant E. coli isolated from a hospitalized patient with acute diarrhea in Thailand.

Identification of novel DNA sequence associated with pathogenicity island III locus in Uropathogenic Escherichia coli isolate and distribution of virulence determinants in β-lactam resistant isolates.

There is an increase in the number of Enterobacteriaceae with Extended-spectrum beta-lactamase (ESBL) type of antibiotic resistance in the Kingdom of Bahrain. However, the virulence determinants of the clinical isolates have not been previously assessed. The current study investigates the distribution of different virulence factors and the phylogenetic groups of antibiotic resistant Escherichia coli isolates in extraintestinal infections.

Separation of cells in density gradients with temporary solidification of the gradient: Application to Escherichia coli forms obtained by microwave treatment of pure cultures.

Gelatin added in certain concentration into the density gradient mixtures allows to solidify and melt the gradient reversibly. Such a method was applied to separation of partially disintegrated Escherichia coli VKM B-125 cells in discontinuous sucrose gradient which was optimized to obtain small round "nano-cells" free from other cell components. Gelatin added to the sucrose solutions had the advantages: i) accurate gradient layers were formed, ii) gelatin did not interfere with the cells and sub-particles ...

Towards patterned bioelectronics: facilitated immobilization of exoelectrogenic Escherichia coli with heterologous pili.

Biosensors detect signals using biological sensing components such as redox enzymes and biological cells. Although cellular versatility can be beneficial for different applications, limited stability and efficiency in signal transduction at electrode surfaces represent a challenge. Recent studies have shown that the Mtr electron conduit from Shewanella oneidensis MR-1 can be produced in Escherichia coli to generate an exoelectrogenic model system with well-characterized genetic tools. However, means to spec...

International high-risk clonal lineages of CTX-M-producing Escherichia coli F-ST648 in free-roaming cats, South America.

The dissemination of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli belonging to high-virulent pandemic lineages has become a global problem with serious consequences for public health worldwide. In this regard, E. coli lineages belonging to the sequence type ST648, which are mostly associated with nosocomial infections, have begun to be reported in animals. In this study, we report the identification and genomic characterization of international CTX-M-producing E. coli ST648/F lineages in...

Identification of several key genes by microarray data analysis of bovine mammary gland epithelial cells challenged with Escherichia coli and Staphylococcus aureus.

This study was aimed at exploring the mechanisms and identifying the key candidate genes associated with S. aureus and E. coli mastitis.

Production of γ-aminobutyric acid in Escherichia coli by engineering MSG pathway.

The compound γ-aminobutyric acid (GABA) has many important physiological functions. The effect of glutamate decarboxylases and the glutamate/GABA antiporter on GABA production was investigated in Escherichia coli. Three genes, gadA, gadB, and gadC were cloned and ligated alone or in combination into the plasmid pET32a. The constructed plasmids were transformed into Escherichia coli BL21(DE3). Three strains, E. coli BL21(DE3)/pET32a-gadA, E. coli BL21(DE3)/pET32a-gadAB and E. coli BL21(DE3)/pET32a-gadABC we...

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