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17:22 EDT 21st July 2018 | BioPortfolio

VITRO DIAGN STICA PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest VITRO DIAGN STICA articles that have been published worldwide.

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Showing "VITRO DIAGN STICA" PubMed Articles 1–25 of 8,300+

La fascitis necrotizante por estrepotococo del grupo A es una infección infrecuente del tejido subcutáneo  y de la fascia, con una tasa de mortalidad elevada debido a su rápida progresión a shock y fallo multiorgánico. Se desarrolla generalmente en extremidades tras un traumatismo o lesión previa. El diagnóstico temprano es esencial así como también el manejo rápido y agresivo. Presentamos un caso de fascitis necrotizante de evolución rápida a pesar del diagnóstico precoz y tratamiento especí...


El cáncer de páncreas es la cuarta causa de muerte por cáncer en los Estados Unidos; en el mundo se asocia con 227.000 muertes anuales, aproximadamente. Es producto de múltiples factores, siendo el tabaquismo el principal factor de riesgo.La punción-aspiración con aguja fina guiada por ultrasonido endoscópico es una técnica muy eficaz en el diagnóstico de lesiones neoplásicas del páncreas. El diagnóstico citológico mediante esta técnica debe hacerse según los lineamientos para el sistema panc...

Ciencias Médicas Universidad Nacional de Córdoba.ResumenLa violencia doméstica es actualmente un problema de salud pública debido a su magnitud y repercusiones sociosanitarias. Objetivo: explorar las características epidemiológicas sociodemográficas y psicopatológicas  de personas denunciadas como agresores en la ciudad de Córdoba. Material y Métodos: estudio exploratorio, retrospectivo y transversal de los registros de personas denunciadas como presuntos agresores de violencia intrafamiliar en l...


Analytical validation of viral CNS Flow Chip kit for detection of acute meningitis and encephalitis.

A new molecular assay (Viral CNS Flow Chip kit, Master Diagnóstica, Spain) has been developed for the detection of eight viruses causing acute meningitis and encephalitis, i.e. herpes simplex viruses 1-2, varicella zoster virus, human enterovirus, human parechovirus, Toscana virus, human cytomegalovirus and Epstein Barr virus. The new assay is a multiplex one-step RT-PCR followed by automatic flow-through hybridization, colorimetric detection and image analysis. The limit of detection was 50 copies/reactio...

Detección de Treponema pallidum subespecie pallidum para el diagnóstico de sífilis congénita mediante reacción en cadena de la polimerasa anidada.

Introducción. La sífilis es una enfermedad producida por Treponema pallidum subespecie pallidum cuya incidencia mundial es de 12 millones de casos por año, aproximadamente; de estos, más de dos millones se presentan en mujeres gestantes, siendo la sífilis congénita la complicación más grave de esta infección en el embarazo.Objetivo. Detectar la presencia de T. pallidum subespecie pallidum en muestras clínicas para el diagnóstico de sífilis congénita mediante reacción en cadena de la polimerasa...

Resumen: La valoración del Índice de Filtrado Glomerular (IFG) es fundamental para la selección de potenciales donantes renales. Para su medición existen métodos poco prácticos y complejos, por lo cual se han desarrollado ecuaciones de estimación del IFG. Objetivo: Evaluar el rendimiento diagnóstico de ecuaciones de estimación del IFG a partir de Creatinina y Cistatina C en una población de adultos sanos candidatos a donantes renales. Materiales y métodos: Se llevó a cabo un estudio transversal ...

El sarcoma de Kaposi es un tumor linfoangioproliferativo descripto por primera vez en el año 1872 por Moritz Kaposi. Hay cuatro variantes clínicas, la forma clásica, la endémica, la iatrogénica asociada a trasplante o inmunosupresión y la epidémica asociada a SIDA, de la cual se hará referencia en esta publicación. Se describe un paciente varón de 31 años, al cual durante la misma internación se hace el diagnóstico de SIDA y de sarcoma de Kaposi.

Thirteen years' experience in fertility preservation for cancer patients after in vitro fertilization and in vitro maturation treatments.

This study aims to describe the experience and outcomes of in vitro maturation without ovarian stimulation (IVM-FP) and conventional in vitro fertilization after ovarian stimulation (IVF-FP) in a fertility preservation (FP) program for women with cancer.

Green Tea Polyphenols Mitigate Gliadin-Mediated Inflammation and Permeability in Vitro.

Green tea, a polyphenol-rich beverage, has been reported to mitigate a number of inflammatory and hypersensitivity disorders in laboratory models, and has been shown to moderate pathways related to food allergies in vitro. We sought to determine the impact of decaffeinated green tea extract (GTE) on the digestion of gliadin protein in vitro and the effect of physical interactions with GTE on the ability of gliadin to stimulate celiac disease-related symptoms in vitro.

Cytokine patterns in vitro, in particular IL-5/IL-8-ratio, to detect patients with nickel contact allergy.

Patch test (PT) is the gold standard to reveal Nickel (Ni) allergy. In vitro tests are under discussion. We aimed to establish a cytokine based in-vitro assay to detect Ni sensitization.

Development of an In Vitro 3D Brain Tissue Model Mimicking In Vivo-Like Pro-inflammatory and Pro-oxidative Responses.

To analyze complex inflammatory responses in an in vitro system, we constructed a new 3D in vitro brain tissue model that exhibits in vivo-like tissue responses (e.g. immune cell phenotypes, and molecular response) to inflammatory stimuli. Finite element modeling of oxygen diffusion and cellular oxygen consumption predicted the oxygen profile within 3D structures, consisting of Type I collagen hydrogel embedded with murine microglia. Viability and cytotoxicity analyses supported the mathematical analysis, d...

A tribute to Dr. Gordon Hisashi Sato (December 24, 1927-March 31, 2017).

Gordon H. Sato, an innovator in mammalian tissue culture and integrated cellular physiology, passed away in 2017. In tribute to Dr. Sato, In Vitro Cellular and Developmental Biology-Animal presents a collection of invited remembrances from six colleagues whose associations with Dr. Sato spanned more than 40 years. Dr. Sato was a past president of the Tissue Culture Association (now the Society for In Vitro Biology), editor-in-chief of In Vitro Cellular and Developmental Biology (1987-1991), and the recipien...

Mimicking brain tissue binding in an in vitro model of the blood-brain barrier illustrates differences between in vitro and in vivo methods for assessing the rate of brain penetration.

Assessing the rate of drug delivery to the central nervous system (CNS) in vitro has been used for decades to predict whether CNS drug candidates are likely to attain their pharmacological targets, located within the brain parenchyma, at an effective dose. The predictive value of in vitro blood-brain barrier (BBB) models is therefore frequently assessed by comparing in vitro BBB permeability, usually quoted as the endothelial permeability coefficient (P) or apparent permeability (P), to their rate of BBB pe...

Do in vitro fertilization treatments result in healthy babies?

In Finland, the proportion of children born as a result of in vitro fertilization treatments is annually approximately 3.3%, and the percentage proportion of the population is growing. Their general somatic health status and cognitive development do not differ from spontaneously fertilized children. In vitro fertilization treatments are, however, associated with a slightly elevated risk of preterm delivery, low birth weight and structural abnormalities. The risk of childhood cancer does not appear to be inc...

Testicular organoids: a new model to study the testicular microenvironment in vitro?

In recent decades, a broad range of strategies have been applied to model the testicular microenvironment in vitro. These models have been utilized to study testicular physiology and development. However, a system that allows investigations into testicular organogenesis and its impact in the spermatogonial stem-cell (SSC) niche in vitro has not been developed yet. Recently, the creation of tissue-specific organ-like structures called organoids has resurged, helping researchers to answer scientific questions...

In Vitro Tests for Aerosol Deposition. VI: Realistic Testing with Different Mouth-Throat Models and In Vitro-In Vivo Correlations for a Dry Powder Inhaler, Metered Dose Inhaler, and Soft Mist Inhaler.

In vitro-in vivo correlations (IVIVC) for lung deposition may be established by testing inhalers in vitro with realistic mouth-throat (MT) models and inhalation profiles (IP). This study was designed to compare the currently available MT models and their ability to predict in vivo lung deposition.

Establishment of intracellular tenofovir-diphosphate as the key determinant for in vitro-in vivo translation of antiviral efficacy.

In vitro evaluation of tenofovir disproxil fumarate (TDF) and tenofovir alafenamide (TAF) revealed comparable antiviral effects with respect to the tenofovir-diphosphate (TFV-DP) level in human peripheral blood mononuclear cells (PBMCs), despite the EC50 values determined based on prodrug concentrations were nearly two orders of magnitude apart. In vivo EC50 obtained from meta-analyses were in good agreement with the in vitro results, indicating intracellular TFV-DP can be employed for in vitro-in vivo tran...

Lysophosphatidic acid increases in vitro maturation efficiency via uPA-uPAR signaling pathway in cumulus cells.

Lysophosphatidic acid (LPA) is a phospholipid-derived signaling molecule with biological activities, such as stimulating cell proliferation, differentiation and migration. In the present study, we examined the effect of LPA on porcine oocytes during in vitro maturation (IVM) and subsequent embryonic development following parthenogenetic activation (PA) and in vitro fertilization (IVF). During IVM, the maturation medium was supplemented with various concentrations of LPA (0, 10, 30, and 60 μM). After 42...

Prediction of Digestible and Metabolizable Energy of Corn Distillers Dried Grains with Solubles (DDGS) for Growing Pigs using in vitro Digestible Nutrients.

The objective of this study was to develop prediction equations (EQS) that estimate DE and ME content of corn distillers dried grains with solubles (DDGS) using digestible (DIG) nutrients from in vitro or in vivo assays. Chemical composition data from 12 sources of corn DDGS were obtained using laboratory chemical analysis (LCA) and near infrared spectroscopy (NIRS). In vitro DM disappearance (IVDMD) from gastric and small intestine hydrolysis (IVDMDh), large intestine fermentation (IVDMDf), and total tract...

In Vitro Analysis of α-Synuclein Amyloid Formation and Cross-Reactivity.

In vitro time-resolved characterization of protein aggregation into amyloid fibers and the effects of other proteins on the aggregation process are fundamentally important measurements to obtain a better understanding of the mechanisms contributing to neurodegeneration, as well as other diseases involving amyloid formation. Here, we describe how to perform in vitro aggregation experiments with α-synuclein, the amyloidogenic protein involved in Parkinson's disease, including how to assess the starting mater...

Prevention of in vitro fertilization twins should focus on maximizing single embryo transfer versus twins are an acceptable complication of in vitro fertilization.

The development of in vitro embryo production in the horse.

The development of techniques to produce equine embryos in vitro is reviewed with specific reference to intracytoplasmic sperm injection (ICSI). Unexplored 50 years ago, this technology has progressed rapidly in the last 20 years to become a commercial reality for the equine breeding industry. Improvements in our understanding of oocyte and embryo competence in the horse have been key factors in overcoming some of the initial problems associated with ICSI. It is now possible to obtain high nuclear maturatio...

Stiffness Matters: Part I - The Effects of Plate Stiffness on the Biomechanics of ACDF In Vitro.

In vitro biomechanical testing of human cadaveric cervical and goat cervical motion segments.

In Vitro Culture of Mouse Oocytes for Meiotic Maturation.

In vitro maturation of mouse oocytes, as well as of many other mammalian species, is used commonly in experimental embryology. This model system gives the possibility to easily obtain maturing oocytes at desired stages of the maturation process. Here we describe how to establish the in vitro culture of maturating mouse oocytes. We focus on the use of oocytes maturation inhibitors to synchronize oocytes at the GVBD stage and on the procedure to distinguish between transcribing and nontranscribing GV-arrested...

Objetivo: Identificar los factores asociados al Síndrome de Burnout (SB) en trabajadores de salud del Perú en el año 2014.Métodos: Estudio transversal analítico mediante un análisis secundario de la Encuesta Nacional de Usuarios de Salud del año 2014 en Perú. El SB fue medido mediante el Maslach Burnout Inventory - Human Services Survey (MBI-HSS). Se realizó un análisis descriptivo, también un análisis bivariado y multivariado calculando los valores de p y los OR crudos y ajustados mediante Regr...


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