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PubMed Journals Articles About "Variably Improved Microbial Source Tracking With Digital Droplet" RSS

19:15 EDT 19th June 2019 | BioPortfolio

Variably Improved Microbial Source Tracking With Digital Droplet PubMed articles on BioPortfolio. Our PubMed references draw on over 21 million records from the medical literature. Here you can see the latest Variably Improved Microbial Source Tracking With Digital Droplet articles that have been published worldwide.

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Showing "Variably improved microbial source tracking with digital droplet" PubMed Articles 1–25 of 15,000+

Variably improved microbial source tracking with digital droplet PCR.

This study addressed whether digital droplet PCR (ddPCR) could improve sensitivity and specificity of human-associated Bacteroidales genetic markers, BacHum and B. theta, and their quantification in environmental and fecal composite samples. Human markers were quantified by qPCR and ddPCR platforms obtained from the same manufacturer. A total of 180 samples were evaluated by each platform including human and animal feces, sewage, and environmental water. The sensitivity of ddPCR and qPCR marker assays in se...


Droplet barcoding: tracking mobile micro-reactors for high-throughput biology.

Droplet microfluidics has become a powerful analytical platform in biological research for conducting high-throughput screening in millions of discrete micro-reactors per hour. While the method facilitates faster and cheaper testing than conventional microtiter plates, the mobile nature of droplets makes micro-reaction tracking a notable challenge. To address this, researchers are developing a variety of tracking methods, ranging from organizing droplets into an index or labeling droplets with a barcode. Th...

An integrated droplet-digital microfluidic system for on-demand droplet creation, mixing, incubation, and sorting.

Droplet microfluidics is a technique that has the ability to compartmentalize reactions in sub nano- (or pico-) liter volumes that can potentially enable millions of distinct biological assays to be performed on individual cells. In a typical droplet microfluidic system, droplets are manipulated by pressure-based flows. This has limited the fluidic operations that can be performed in these devices. Digital microfluidics is an alternative microfluidic paradigm with precise control and manipulation over indiv...


Droplet volume variability and impact on digital PCR copy number concentration measurements.

Digital polymerase chain reaction (dPCR) is increasingly being adopted by reference material producers and metrology insti-tutes for value assignment, and for homogeneity and stability studies of nucleic acid reference materials. A reference meth-od procedure should fulfil several requirements, and the uncertainty and biases should be completely understood. A bias in target concentration when inaccurate droplet volume is used in the droplet dPCR measurement equation has previously been documented. In this s...

Semiconductor-laser-based hybrid chaos source and its application in secure key distribution.

An analog-digital hybrid optical chaos source and a corresponding secure key distribution (SKD) scheme are proposed. An analog-digital hybrid electro-optic feedback loop is introduced to enhance the robustness of the chaotic semiconductor lasers. The source, which can adopt robust digital synchronization strategies, could generate a broadband analog optical chaotic signal of high dynamical complexity. Furthermore, the source reduces the requirement on the processing speed of digital components and simplifie...

Tracking seasonal rhythms of plants in diverse ecosystems with digital camera imagery.

Global change is shifting the seasonality of vegetation in ecosystems around the globe. High-frequency digital camera imagery, and vegetation indices derived from that imagery, is facilitating better tracking of phenological responses to environmental variation. This method, commonly referred to as the "phenocam" approach, is well-suited to several specific applications, including: close-up observation of individual organisms; long-term canopy-level monitoring at individual sites; automated regional-to-cont...

Evaluation of the analytical and diagnostic performance of a digital droplet polymerase chain reaction (ddPCR) assay to detect Trypanosoma cruzi DNA in blood samples.

The recent development of novel Polymerase Chain Reaction (PCR) technologies that confer theoretical advantages over quantitative PCR has considerable potential in the diagnosis of low load infections, such as Trypanosoma cruzi in the chronic phase of Chagas disease. We evaluated the utility of the digital droplet (dd)PCR platform in the detection of T. cruzi infection.

Digital droplet polymerase chain reaction analysis of common viruses in the aqueous humour of patients with Posner-Schlossman syndrome in Chinese population.

To compare the detection results consistency of quantitative polymerase chain reaction (qPCR) and digital droplet polymerase chain reaction (ddPCR), and determine the value of ddPCR for viral detection in the aqueous humour.

Non-invasive prenatal diagnosis for beta-thalassemia by detecting paternal CD41-42 mutation in cell-free DNA derived from maternal plasma with droplet digital PCR.

To establish a non-invasive method for beta-thalassemia by detecting parental CD41-42 mutation in cell-free DNA derived from maternal plasma with droplet digital PCR (ddPCR).

Extended direct lysis method for virus detection on berries including droplet digital RT-PCR or real time RT-PCR with reduced influence from inhibitors.

Detection of viruses on berries is a challenging task, often hampered by the presence of RT-qPCR inhibiting substances from berry juice. A direct extraction method for virus detection (murine norovirus and GA phage) on frozen raspberries was previously published. We expanded (different types of berries and viruses) and improved the method using MobiSpin S400 columns that filter nucleic acids based on size-exclusion chromatography. While no inhibition was detected in filtered RNA, unfiltered RNA needed from ...

Statistical Analysis of Non-Uniform Volume Distributions for Droplet-based Digital PCR Assays.

We present a method to determine the concentration of nucleic acids in a sample by partitioning it into droplets with a non-uniform volume distribution. This digital PCR method requires no special equipment for partitioning, unlike other methods that require nearly identical volumes. Droplets are generated by vortexing a sample in an immiscible oil to create an emulsion. PCR is performed and droplets in the emulsion are imaged. Droplets with one or more copies of a nucleic acid are identified and the nuclei...

Fecal source tracking based on fecal coliform concentration and bacterial community structure in the Bong stream, Korea.

Fecal source tracking of the Bong stream, a representative inland pollutant around the drainage basin of Gangjin Bay (an area where shellfish are grown for export), was performed three times in four confluence areas with 13 sampling sites by analyzing fecal coliform concentrations and two types of bacterial community structures. Identification of the origin of major fecal pollution in the area that inflowed simultaneously via several branch streams was difficult using fecal source tracking based on fecal co...

Antifouling digital microfluidics using lubricant infused porous film.

Electrowetting-driven digital (droplet-based) microfluidics has a tremendous impact on lab-on-a-chip applications. However, the biofouling problem impedes the real applications of such digital microfluidics. Here we report antifouling digital microfluidics by introducing lubricant infused porous film to electrowetting (more exactly, electrowetting on dielectric or EWOD). Such film minimizes direct contact between droplets and the solid surface but provides liquid-liquid contact between droplets and the lubr...

An ultrasensitive test for profiling circulating tumor DNA using integrated comprehensive droplet digital detection.

Current cancer detection systems lack the required sensitivity to reliably detect minimal residual disease (MRD) and recurrence at the earliest stages when treatment would be most effective. To address this issue, we present a novel liquid biopsy approach that utilizes an integrated comprehensive droplet digital detection (IC3D) digital PCR system which combines microfluidic droplet partitioning, fluorescent multiplex PCR chemistry, and our rapid 3D, large-volume droplet counting technology. The IC3D ddPCR ...

Superimposition of 3D maxillary digital models using open-source software.

Historically, whether for research purposes or clinical monitoring, orthodontic evaluation of dental movements has been done using plaster study models and two dimensional (2D) radiographs. However, new frontiers for the diagnosis, planning and outcome assessment of orthodontic treatments have arisen, due to the revolutionary digital tools which enable a three dimensional (3D) computerized analysis of dental movements by means of digital models. However, the software for 3D analysis are often costly, result...

Characterization and source-tracking of antibiotic resistomes in the sediments of a peri-urban river.

The peri-urban rivers are one of the critical interfaces between urban-rural symbiotic ecosystems and appear to be a reservoir of antibiotic resistance genes (ARGs) in the environment. To prevent the transmission risks of ARGs between peri-urban river and human, it is essential to explore the prevalence and source of ARGs in the environment for designing potential mitigation strategies. In this study, we focused on the characterization and source-tracking of ARGs in the sediments of a typical peri-urban riv...

Assessment on applicability of common source tracking tools for particulate organic matter in controlled end member mixing experiments.

In this study, ideal mixing behaviors of the three commonly-used source tracking tools, which include fluorescence spectroscopy, stable isotope ratios, and lipid biomarkers, were evaluated in controlled particular organic matter (POM) mixtures of two contrasting end members e.g., soil and algae. In parallel, three different extraction methods based on water (WEOM) and two alkaline solutions were compared to identify the preferential pre-treatment option that leads to a good performance of the spectroscopic ...

Aripiprazole with digital ingestion tracking (Abilify MyCite).

A new droplet-breakup phenomenon in electrokinetic flow through a microchannel constriction.

A completely new droplet breakup phenomenon is reported for droplets passing through a constriction in an electrokinetic flow. The breakup occurs during the droplet shape recovery process past the constriction throat by the interplay of the dielectrophoretic (DEP) stress release and the interface energy for droplets with smaller permittivity than that of the ambient fluid. There are conditions for constriction ratios an droplet size that the droplet breakup occurs. The numerical predictions provided here re...

Development of a droplet digital PCR assay for sensitive detection of porcine circovirus 3.

Porcine circovirus 3 (PCV3), a newly emerged circovirus, is associated with porcine dermatitis and nephropathy syndrome, reproductive failure and multi-systemic inflammation disease, and is widely distributed in pig populations worldwide. Therefore, developing specific diagnostic assays will be important for controlling this emerging pathogen. In this study, we developed a novel droplet digital PCR (ddPCR) assay targeting the PCV3 cap gene to improve the sensitivity of PCV3 detection. The established assay ...

Velocity Saturation in Digital Microfluidics.

In digital microfluidics, discrete droplets of fluid are made to move on an open surface with no microchannels. These systems are commonly operated by application of electrical driving forces to an array of electrodes. While these driving forces are well characterized, the dissipative forces opposing droplet movement have not been as thoroughly examined. In recognition of this deficit, we used force-velocity plots to characterize droplet movement in digital microfluidics, which was found to be consistent wi...

Source Environments of the Microbiome in Perennially Ice-Covered Lake Untersee, Antarctica.

Ultra-oligotrophic Lake Untersee is among the largest and deepest surface lakes of Central Queen Maud Land in East Antarctica. It is dammed at its north end by the Anuchin Glacier and the ice-cover dynamics are controlled by sublimation - not melt - as the dominating ablation process and therefore surface melt during austral summer does not provide significant amounts of water for recharge compared to subsurface melt of the Anuchin Glacier. Several studies have already described the structure and function o...

BrachyView: initial preclinical results for a real-time in-body HDR PBT source tracking system with simultaneous TRUS image fusion.

A prototype in-body gamma camera system with integrated trans-rectal ultrasound (TRUS) and associated real-time image acquisition and analysis software was developed for intraoperative source tracking in high dose rate (HDR) brachytherapy. The accuracy and temporal resolution of the system was validated experimentally using a deformable tissue-equivalent prostate gel phantom and a full clinical HDR treatment plan. The BrachyView system was able to measure 78% of the 200 source positions with an accuracy of ...

Determination of copy number and circularization ratio of Tn-Tn family of conjugative transposons in oral streptococci by droplet digital PCR.

Tn916 and Tn1545 are paradigms of a large family of related, broad host range, conjugative transposons that are widely distributed in bacteria and contribute to the spread of antibiotic resistance genes (ARGs). Variation in the copy number (CN) of Tn916-Tn1545 elements and the circularization ratio (CR) may play an important role in propagation of ARGs carried by these elements. In this study, the CN and CR of Tn916-Tn1545 elements in oral streptococci were determined using droplet digital PCR (ddPCR). In ...

Geometrically invariant watermarking for medical images based on digital holography and three-level DWT-SVD.

Digital imaging and communications in medicine are being applied widely to improve health care for people, and their security attracted our attention when they are transmitted through the Internet. The paper proposes a digital watermarking method for medical images based on digital holography and three-level discrete wavelength transform (DWT) singular value decomposition (SVD), where a live code including privacy information is transformed into digital holography by an improved computer-generated hologram ...


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