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PubMed Journals Articles About "Algorithm Simultaneous Cell Diameter Intensity Quantification Resolution Imaging" RSS

14:19 EDT 14th October 2019 | BioPortfolio

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Showing "algorithm simultaneous cell diameter intensity quantification resolution imaging" PubMed Articles 1–25 of 40,000+

H-EM: An algorithm for simultaneous cell diameter and intensity quantification in low-resolution imaging cytometry.

Fluorescent cytometry refers to the quantification of cell physical properties and surface biomarkers using fluorescently-tagged antibodies. The generally preferred techniques to perform such measurements are flow cytometry, which performs rapid single cell analysis by flowing cells one-by-one through a channel, and microscopy, which eliminates the complexity of the flow channel, offering multi-cell analysis at a lesser throughput. Low-magnification image-based cytometers, also called "cell astronomy" syste...


Three-dimensional high-resolution simultaneous quantitative mapping of the whole brain with 3D-QALAS: An accuracy and repeatability study.

Previous methods for the quantification of brain tissue properties by magnetic resonance imaging were mainly based on two-dimensional acquisitions and were thus limited to a relatively low resolution in the slice direction compared to three-dimensional (3D) acquisitions. The 3D-quantification using an interleaved Look-Locker acquisition sequence with a T2 preparation pulse (3D-QALAS) sequence may allow for simultaneous acquisition of relaxometry parameters in high spatial resolution.

Image-based assessment of uncertainty in quantification of carotid lumen.

Measurements of the vessel lumen diameter are often used to determine the degree of atherosclerotic disease in carotid arteries. However, quantification results vary with imaging technique and acquisition settings. We aim at providing a tool that quantifies the lumen diameter on different image datasets and gives an estimate of quantification uncertainties, so that they can be taken into consideration when evaluating and comparing measurements. For the segmentation of the vessel lumen, we present an algorit...


Method for spike detection from microelectrode array recordings contaminated by artifacts of simultaneous two-photon imaging.

The simultaneous utilization of electrophysiological recordings and two-photon imaging allows the observation of neural activity in a high temporal and spatial resolution at the same time. The three dimensional monitoring of morphological features near the microelectrode array makes the observation more precise and complex. In vitro experiments were performed on mice neocortical slices expressing the GCaMP6 genetically encoded calcium indicator for monitoring the neural activity with two-photon microscopy a...

Fluorescent property of glycol chitosan-fluorescein isothiocyanate conjugate for bio-imaging material.

Bio-imaging is an effective method for visualizing the biological responses and pharmacological responses in living organisms. In general, bio-imaging materials stain the cell membrane through electrostatic interaction and hydrogen bonding. However, the current commercial bio-imaging materials have several disadvantages, such as weak intensity, poor resolution, and high price. In this study, a novel bio-imaging material with high fluorescent intensity and water solubility was prepared using glycol chitosan....

Multi-focus microscope with HiLo algorithm for fast 3-D fluorescent imaging.

In this paper, we present a new multi-focus microscope (MFM) system based on a phase mask and HiLo algorithm, achieving high-speed (20 volumes per second), high-resolution, low-noise 3-D fluorescent imaging. During imaging, the emissions from the specimen at nine different depths are simultaneously modulated and focused to different regions on a single CCD chip, i.e., the CCD chip is subdivided into nine regions to record images from the different selected depths. Next, HiLo algorithm is applied to remove t...

Free-running simultaneous myocardial T1/T2 mapping and cine imaging with 3D whole-heart coverage and isotropic spatial resolution.

To develop a free-running framework for 3D isotropic simultaneous myocardial T1/T2 mapping and cine imaging.

Simultaneous T mapping of N- and N-labeled dicarboxy-PROXYLs using CW-EPR-based single-point imaging.

This article reports a method of simultaneous T mapping of N- and N-labeled dicarboxy-PROXYLs using 750-MHz continuous-wave electron paramagnetic resonance (CW-EPR) imaging. To separate the spectra of N- and N-labeled dicarboxy-PROXYLs under magnetic field gradients, an optimization problem for spectral projections was formulated with the spatial total variation as a regularization term and solved using a local search based on the gradient descent algorithm. Using the single-point imaging (SPI) method with ...

Simultaneous intraluminal imaging of tissue autofluorescence and eGFP-labeled cells in engineered vascular grafts inside a bioreactor.

The growing demand for tissue engineered vascular grafts (TEVG) motivates the development of optimized fabrication and monitoring procedures. Bioreactors which provide physiologically-relevant conditions are important for improving holistic TEVG properties and performance. Herein we describe a fiber-based intraluminal imaging system that allows for in-situ assessment of vascular materials and re-cellularization processes inside a bioreactor by simultaneous and co-registered measurements of endogenous fluore...

The relationship between biomechanics of pharyngoesophageal segment and tracheoesophageal phonation.

This study examined the relationship between biomechanical features of the pharyngoesophageal (PE) segment, acoustic characteristics of tracheoesophageal (TE) phonation, and patients' satisfaction with TE phonation. Fifteen patients using TE phonation after total laryngectomy completed the Voice Symptom Scale (VoiSS) and underwent acoustic voice analysis for cepstral peak prominence (CPP) and relative intensity. High resolution manometry (HRM) combined with videofluoroscopy was used to evaluate PE segment p...

Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing.

Probing remote matter with laser light is a ubiquitous technique used in circumstances as diverse as laser-induced breakdown spectroscopy and barcode scanners. In classical optics, the intensity that can be brought to bear on a remote target is limited by the spot size of the laser at the distance of the target. This spot size has a lower bound determined by the diffraction limit of classical optics. However, amplified femtosecond laser pulses generate intensity sufficient to modify the refractive index of ...

Methods for analyzing tellurium imaging mass cytometry data.

Imaging mass cytometry (IMC) is a technique allowing visualization and quantification of over 40 biological parameters in a single experiment with subcellular spatial resolution, however most IMC experiments are limited to endpoint analysis with antibodies and DNA stains. Small molecules containing tellurium are promising probes for IMC due to their cell permeability, synthetic versatility, and most importantly their application to sequential labelling with isotopologous probes (SLIP) experiments. SLIP expe...

High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the Host.

In vivo imaging, meaning imaging tissues in living animals, is still a developing technique. However, microscopy imaging ex vivo remains a very important tool that allows for visualization of biological and pathological processes occurring in vivo. As described in Chap. 5, imaging of animal and human tissue postmortem can be performed at high resolution. Recently, imaging of human tissues infected with pneumococci using an even higher resolution, the so-called super-resolution with STED, has been reported.

Efficient glare suppression with Hadamard-encoding-algorithm-based wavefront shaping.

In this Letter, we explore the effectiveness of a Hadamard encoding algorithm (HEA) for efficiently suppressing glare. Both numerical simulations and experimental data show that light intensity decays exponentially with respect to the number of HEA measurements. Specifically, we applied the HEA to reduce the intensity of a single speckle to 4.1% of its original value within only 16 measurements. In contrast, the commonly used genetic algorithm (GA) can reduce the speckle intensity only to 0.2 of its origina...

Plaque components segmentation in carotid artery on simultaneous non-contrast angiography and intraplaque hemorrhage imaging using machine learning.

This study sought to determine the feasibility of using Simultaneous Non-contrast Angiography and intraPlaque Hemorrhage (SNAP) to detect the lipid-rich/necrotic core (LRNC), and develop a machine learning based algorithm to segment plaque components on SNAP images.

Quantifying the Training-Intensity Distribution in Middle-Distance Runners: The Influence of Different Methods of Training-Intensity Quantification.

To compare the training-intensity distribution (TID) across an 8-wk training period in a group of highly trained middle-distance runners employing 3 different methods of training-intensity quantification.

A Geant4 simulation for three-dimensional proton imaging of microscopic samples.

Proton imaging can be carried out on microscopic samples by focusing the beam to a diameter ranging from a few micrometers down to a few tens of nanometers, depending on the required beam intensity and spatial resolution. Three-dimensional (3D) imaging by tomography is obtained from proton transmission (STIM: Scanning Transmission Ion Microscopy) and/or X-ray emission (PIXE: Particle Induced X-ray Emission). In these experiments, the samples are dehydrated for under vacuum analysis. In situ quantification o...

Strategies to maximize performance in STimulated Emission Depletion (STED) nanoscopy of biological specimens.

Super-resolution fluorescence microscopy has become an important catalyst for discovery in the life sciences. In STimulated Emission Depletion (STED) microscopy, a pattern of light drives fluorophores from a signal-emitting on-state to a non-signalling off-state. Only emitters residing in a sub-diffraction volume around an intensity minimum are allowed to fluoresce, rendering them distinguishable from the nearby, but dark fluorophores. STED routinely achieves resolution in the few tens of nanometers range i...

A simulation study of a fan-beam time-of-flight fast-neutron tomography system.

A fast neutron imaging system, consisting of a deuterium-tritium neutron generator, an associated alpha-particle detector and an arc-shaped array of individual neutron detectors is simulated for fan-beam fast neutron tomography purpose. In this system, the associated particle and time of flight techniques are used to separate transmission neutrons from scattered neutrons by electronically collimating and time tagging the source neutrons. To evaluate factors affecting the system's spatial resolution and imag...

Single-Cell Live Imaging.

Recent fluorescence microscopy allows for high-throughput acquisition of 5D (X, Y, Z, T, and Color) images in various targets such as cultured cells, 3D spheroid/organoid, and even living tissue with single-cell resolution. The technology is considered promising to augment insights on heterogeneous features of both physiological and pathological cell phenotypes, for instance, distinct responses of cancer cells to anticancer drug treatment. Here we overview microscopic applications to capture live cell event...

A qHNMR method for simultaneous quantification of terpenoids from Ferula ovina (Boiss.) Boiss roots.

Ferula ovina (Boiss.) Boiss is one of the most important endemic medicinal plants in Iran, which has three main terpenoid compounds including ferutinin, stylosin and tschimgine. Ferutinin is the strongest natural phytoestrogen that has agonistic activity on estrogen receptors, particularly α-receptors. To determine the amount of ferutinin in F. ovina roots, we firstly used HPLC-UV method. In the HPLC method, the resolution of ferutinin from the two other compounds, stylosin and tshimgine, was poor. Therefo...

Spectrometry analysis algorithm based on R-L deconvolution and fuzzy inference.

A spectrometry analysis algorithm based on Richardson-Lucy (R-L) deconvolution and fuzzy inference is developed to identify radionuclides of overlapping spectra obtained by a low-resolution detector. The effectiveness of the algorithm was experimentally verified. It is shown that the algorithm can be used for radionuclide analysis of low-resolution spectra in a marine environment with high accuracy of nuclide identification.

Phase retrieval with extended field of view based on continuous phase modulation.

In order to address two main obstacles that affect the practical application of coherent modulation imaging (CMI)i.e., the low signal-to-noise ratio (SNR) and limited field of view (FOV), a new algorithm providing extended FOV based on CMI is proposed. A weak scattering modulator was used instead of a binary random phase modulator with strong scattering ability, to improve the final resolution of CMI combined with probe scanning. An unlimited FOV was achieved in a noniterative manner, resulting in a superio...

Simultaneous Quantification of the Interplay Between Molecular Turnover and Cell Mechanics by AFM-FRAP.

Quantifying the adaptive mechanical behavior of living cells is essential for the understanding of their inner working and function. Yet, despite the establishment of quantitative methodologies correlating independent measurements of cell mechanics and its underlying molecular kinetics, explicit evidence and knowledge of the sensitivity of the feedback mechanisms of cells controlling their adaptive mechanics behavior remains elusive. Here, a combination of atomic force microscopy and fluorescence recovery a...

Application of the optimized and validated LC-MS method for simultaneous quantification of tryptophan metabolites in culture medium from cancer cells.

Kynurenine pathway is the main route of tryptophan degradation generating a number of immunoregulatory compounds. Some conditions like oxidative stress, inflammatory factors might enhance tryptophan degradation. Process is active in several cells including fibroblasts, cancer cells, and immune cells, therefore it is intensively studied in context of cancer microenvironment. The validated and standardized methodology for kynurenine quantification is crucial for reliable comparison of results obtained in diff...


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