An in Vitro Evaluation of Antimicrobial Photodynamic Therapy on Deciduous Carious Dentin

2015-06-25 05:38:23 | BioPortfolio


Despite of the decline of dental caries, this disease is still common in polarized groups affecting children. Therefore, the aim of this randomized blind in vivo study was to compare the antimicrobial effect of two antimicrobial therapies in deciduous carious dentin. Thirty two patients aging from 5 to 7 years old had partial caries removal in deep carious dentin lesion in deciduous molar and were divided in three groups: Control - chlorhexidine and resin modified glass ionomer cement (RMGIC); LEDTB - antimicrobial photodynamic therapy (APDT) with LED associated with toluidine blue orto dye solution and RMGIC; LMB - APDT with laser associated with methylene blue dye solution and RMGIC. The patients were submitted to initial clinical and radiographic examination and demographic features were evaluated by biofilm, gingival, and dmft/dmfs indexes, besides clinical and radiographic follow up at 6 and 12 months after the treatments. The carious dentin was collected before and after each treatment and the number of Streptococcus mutans, Streptococcus sobrinus, Lactobacillus casei, Fusobacterium nucleatum, Atopobium rimae and total bacteria were established by quantitative PCR.

Study Design

Allocation: Randomized, Intervention Model: Parallel Assignment, Masking: Single Blind (Subject), Primary Purpose: Treatment


Dental Caries


Rubber dam isolation, Tooth drying, Dentin wash with chlorhexidine 2%, Antimicrobial Photodynamic Therapy 1, Antimicrobial Photodynamic Therapy 2




University of Sao Paulo

Results (where available)

View Results


Published on BioPortfolio: 2015-06-25T05:38:23-0400

Clinical Trials [798 Associated Clinical Trials listed on BioPortfolio]

Effect of Photodynamic Therapy on Deep Caries in Permanent Tooth,A Controlled Clinical Trial

The aim of the proposed study is to assess the clinical effect of photodynamic therapy (PDT)using LED on dentin with carious lesions in permenent teeth. Patients with molars exhibiting dee...

Effectiveness of Rubber Dam Isolation Versus Cotton Roll Isolation on Bonded Fissure Sealant Retention

The purpose of this study is to evaluate and compare the clinical retention of bonded fissure sealants placed under rubber dam isolation and cotton roll isolation.

Isolation of the Operative Field Influences the Survival Rate of Composite Restorations

The aim of this study was to evaluate the survival of direct composite resin restorations in primary molars using different methods of isolation of the operative field: absolute isolation ...

Microbial Sampling of Carious Dentin

The School of Dentistry is seeking to determine whether viable microorganisms remain within tooth structure after conventional, mechanical removal of areas of tooth decay, prior to placeme...

"Clinical Trials to Enhance Elders' Oral Health" ("TEETH")

The purpose of this study is to determine if regular rinsing with chlorhexidine is effective as a long-term preventive method for reducing the incidence of tooth loss in low income older ...

PubMed Articles [2413 Associated PubMed Articles listed on BioPortfolio]

New Bone Formation Using an Extracted Tooth as a Biomaterial: A Case Report with Histologic Evidence.

This case report aims to demonstrate the regenerative potential of particles obtained from a crushed extracted tooth. Following tooth removal, the clean root was ground and the dentin and cementum gra...

Chlorhexidine Allergy: On the Rise and Often Overlooked.

In recent years, the risk of allergy to chlorhexidine is increasingly recognised. In this review, we discuss why the allergy is so easily overlooked and point out several preventative initiatives that...

Chlorhexidine sensitivity in staphylococci isolated from patients with central line-associated bloodstream infection.

Since 2011, 2% chlorhexidine in 70% isopropyl alcohol (2% chlorhexidine tincture) has been widely used in Korea. To investigate changes in chlorhexidine sensitivity of staphylococci causing central li...

Bond stability of conventional adhesive system with MMP inhibitors to superficial and deep dentin.

To evaluate the microshear bond strength (μSBS) to deep (DD) or superficial (SD) dentin (μSBS) overtime, nanoleakage (AG%), degree of conversion (DC%), water sorption (WSp), and solubility (WSl) of ...

Proteomics and N-Glycoproteomics analysis of an extracellular matrix-based scaffold-human treated dentin matrix.

Extracellular matrix (ECM)-based biomaterials developed from mammalian tissues have been successfully used in preclinical and clinical tissue engineering applications. We have previously reported abou...

Medical and Biotech [MESH] Definitions

Adherent debris produced when cutting the enamel or dentin in cavity preparation. It is about 1 micron thick and its composition reflects the underlying dentin, although different quantities and qualities of smear layer can be produced by the various instrumentation techniques. Its function is presumed to be protective, as it lowers dentin permeability. However, it masks the underlying dentin and interferes with attempts to bond dental material to the dentin.

An autosomal dominant disorder of tooth development characterized by opalescent dentin resulting in discoloration of the teeth, ranging from dusky blue to brownish. The dentin is poorly formed with an abnormally low mineral content; the pulp canal is obliterated, but the enamel is normal. The teeth usually wear down rapidly, leaving short, brown stumps. (Dorland, 27th ed)

The process of TOOTH formation. It is divided into several stages including: the dental lamina stage, the bud stage, the cap stage, and the bell stage. Odontogenesis includes the production of tooth enamel (AMELOGENESIS), dentin (DENTINOGENESIS), and dental cementum (CEMENTOGENESIS).

Sheets of latex rubber punched and placed over the teeth during dental procedures to isolate the field of operation from the rest of the oral cavity (Jablonski; Illustrated Dictionary of Dentistry, 1982). Rubber dams are useful in preventing the swallowing of instruments or restorations during dental work.

An apparently hereditary disorder of dentin formation, marked by a normal appearance of coronal dentin associated with pulpal obliteration, faulty root formation, and a tendency for peripheral lesions without obvious cause. (From Dorland, 27th ed)

More From BioPortfolio on "An in Vitro Evaluation of Antimicrobial Photodynamic Therapy on Deciduous Carious Dentin"

Quick Search

Relevant Topics

Dentistry is the study, management and treatment of diseases and conditions affecting the mouth, jaw, teeth and their supporting tissues (Oxford Medical Dictionary) The work of a dentist ranges from regular patient check-up to orthodontics and surgery....

Pediatrics is the general medicine of childhood. Because of the developmental processes (psychological and physical) of childhood, the involvement of parents, and the social management of conditions at home and at school, pediatrics is a specialty. With ...

Polymerase Chain Reaction (PCR)
PCR (Polymerase Chain Reaction) uses the ability of DNA polymerase (enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two ident...

Searches Linking to this Trial