Comparison of Biofilm Characteristics Between an in Situ Biofilm Model of Supragingival Plaque and Natural Teeth

2016-05-12 00:38:21 | BioPortfolio


The purpose of this study is to determine in situ the bacterial viability, thickness and bacterial diversity of a biofilm formed on different substrates in comparison to a naturally tooth-formed oral biofilm. In addition, it will be evaluated whether the restraint of oral hygiene measures may influence the development of the PL-Biofilm.


- To calculate an a priori sample size, the following statistical criteria were established: an effect size of 0.35, an alpha error of 0.05 and a statistical power of 87%. Assuming these criteria and using the repeated measures ANOVA test, a sample size of 15 subjects was required.

- Bacterial viability and thickness will be analyse by Confocal Laser Scanning Microscope and Live/Dead BackLight staining solution.

- Bacterial diversity will be analyse by Pyrosequencing the 16S DNAr gene.

Study Design

Allocation: Randomized, Intervention Model: Crossover Assignment, Masking: Double Blind (Investigator, Outcomes Assessor), Primary Purpose: Basic Science






Univeristy of Santiago de Compostela, Faculty of Dentistry
Santiago de Compostela
Galicia/ A Coruña




University of Santiago de Compostela

Results (where available)

View Results


Published on BioPortfolio: 2016-05-12T00:38:21-0400

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