Topics

Lysine Metabolic Availability in Children

2019-10-28 13:57:21 | BioPortfolio

Summary

This study will evaluate how much of the essential amino acid lysine school-aged children are using for protein synthesis when consuming different cereal grains. Six healthy children between 6-10y will be recruited. They will be given cooked white rice, corn, oats, black beans, and milk. Using a minimally invasive technique, the amount of lysine that is available from cereal grain products will be determined. With the results from this research project accurate diet recommendations will be developed for children consuming cereal-based diets.

Description

Purpose The purpose of this research project is to implement the minimally invasive Indicator Amino Acid Oxidation (IAAO) method in healthy school-aged children to measure the metabolic availability of the essential amino acid lysine obtained from commonly consumed cereal grains and their breakfast counterparts. Additionally, the difference in metabolic availability through complementation of breakfast cereals with milk will be assessed.

Hypothesis The use of the IAAO method will yield a higher lysine metabolic availability for those cereal grains that have the least amount of fibre in the food matrix. Combining the consumption of cereals with milk will increase the metabolic availability of lysine.

Justification Cereal grains and their derived products are the most commonly consumed food group around the world. From traditional foods such as steamed rice, corn tortillas, and wheat bread to relatively modern products like ready-to-eat breakfast cereals. Up to 80% of the energy intake in developing countries comes from this food group. Given that they are considered as a low protein quality food source, a considerable amount of the world's population could potentially be at risk of lysine deficiency. Especially, children living in underdeveloped countries where complementation with other food sources, such as legumes, dairy, or other animal products, is not an available option. Since lysine is the limiting amino acid in cereals, this study is of great importance not only for its nutritional value, but also for its social and economic impact.

Current lysine recommendations for school-aged children are based on factorial calculations performed on results of adult male studies. To develop the Lysine EAR (Estimated Average Requirement) for growing children, these studies use adult lysine maintenance requirements multiplied by estimated rates of protein deposition and the efficiency of this deposition. Additionally, these studies do not measure lysine's bioavailability, which will vary depending on the food sources and the processing techniques used to preserve them. With these limitations in mind, the IAAO method has the capacity to accurately measure amino acid metabolic availability in different populations groups, such as healthy and malnourished children. Metabolic availability refers to the amount of absorbed amino acids that are used for protein synthesis. It takes into account all amino acid losses from digestion, absorption, metabolic utilization, and endogenous losses.

Previously, this method was used to determine lysine metabolic availability of rice in healthy adults. This is the only study of this nature done for any human food source. Currently, there is no information available regarding lysine's metabolic availability from corn, wheat, milk and any breakfast cereal. Previously the IAAO method has successfully been implemented in healthy Canadian and Indian children to measure lysine requirements. Additionally, lysine requirement studies has been preformed in moderately undernourished Indian children with parasitic intestinal infections. The study conclusions were that, undernourished children require around 20% more lysine than their healthy counterparts. In these cases it is not only important to know the lysine content of a food source, but also it's metabolic availability, to develop accurate interventions that will have a better chance to meet children's daily requirements.

Given that childhood is a critical period for optimal growth and development, a comprehensive study of lysine's metabolic availability in commonly consumed cereal grains is needed to develop adequate interventions and recommendations, targeted to prevent stunting and other signs of malnourishment in at risk populations around the world.

Objective The general objective of this research project is to assess the degree in which cereal food sources can meet lysine amino acid requirements in healthy school-aged children.

The specific objective us to:

- Determine the metabolic availability of lysine from rice, corn, oats, black beans and milk using the IAAO method in healthy school-aged children.

Research Design This is a repeated measures study design involving 1 pre-study visit and 8 study visits approximately one week apart from each other.

Study Design

Conditions

Children

Intervention

L-Amino Acid

Location

BC Children's Hospital Research Institute
Vancouver
British Columbia
Canada
V5Z4H4

Status

Recruiting

Source

University of British Columbia

Results (where available)

View Results

Links

Published on BioPortfolio: 2019-10-28T13:57:21-0400

Clinical Trials [1838 Associated Clinical Trials listed on BioPortfolio]

Protein Requirements for Active Children

Nutritional requirements for dietary amino acids in children have traditionally been determined utilizing the nitrogen balance technique, which is prone to underestimating protein requirem...

Amino Acid Feed Children's Study

This study of the tolerance and acceptability of an amino acid based feed will assess gastrointestinal (GI) tolerance, product intake and acceptability in relation to taste, smell, texture...

Rate-limiting Amino Acids in Endurance-trained Athlete

Protein requirements in individuals who participate in endurance-based exercise training have been suggested to be greater than the current recommended dietary allowance (RDA). The biolog...

Retrospective Review of Amino Acid Formula Use at a Children's Center

This is a retrospective review of data documented in medical records.

Determination of Pre-Absorptive Dissociation of Zinc From a Zinc Amino Acid Complex in Healthy Men

Zinc may be absorbed from diet via zinc transporter mediated pathways, or, when coupled with amino acids, via amino acid transporter pathways. When zinc is coupled with amino acids in diet...

PubMed Articles [14501 Associated PubMed Articles listed on BioPortfolio]

The RNA-Binding Protein YBX3 Controls Amino Acid Levels by Regulating SLC mRNA Abundance.

Sufficient amino acid supplies are critical for protein synthesis and, thus, cell growth and proliferation. Specialized transporters mediate amino acid exchange across membranes and their regulation i...

Asymmetric Synthesis of α-Amino Phosphonic Acids Using Stable Imino Phosphonate as a Universal Precursor.

A practical method for synthesizing chiral α-amino phosphonic acid derivatives was developed. Readily available and stable N-o-nitrophenylsulfenyl (Nps) imino phosphonate was utilized as a substrate ...

Synthesis of Chiral β-Fluoroalkyl β-Amino Acid Derivatives via Palladium-catalyzed Hydrogenation.

An enantioselective palladium-catalyzed hydrogenation of β-fluoroalkyl-β-amino acrylic acid derivatives has been successfully developed, providing the corresponding chiral β-fluoroalky β-amino aci...

Hydrogen bonding and protonation effects in amino acids' anthraquinone derivatives - Spectroscopic and electrochemical studies.

Six novel amino acid chromophores were synthesized and their spectroscopic, acid-base, and electrochemical properties are discussed in this work. In studied compounds, selected amino acid residues (l-...

HLA alleles, especially amino-acid signatures of HLA-DPB1, might contribute to the molecular pathogenesis of early-onset autoimmune thyroid disease.

The major histocompatibility complex region has been suggested to play an important role in the development of autoimmune thyroid disease (AITD). In this study, we investigated the associations of hum...

Medical and Biotech [MESH] Definitions

Drugs used for their actions on any aspect of excitatory amino acid neurotransmitter systems. Included are drugs that act on excitatory amino acid receptors, affect the life cycle of excitatory amino acid transmitters, or affect the survival of neurons using excitatory amino acids.

(2S-(2 alpha,3 beta,4 beta))-2-Carboxy-4-(1-methylethenyl)-3-pyrrolidineacetic acid. Ascaricide obtained from the red alga Digenea simplex. It is a potent excitatory amino acid agonist at some types of excitatory amino acid receptors and has been used to discriminate among receptor types. Like many excitatory amino acid agonists it can cause neurotoxicity and has been used experimentally for that purpose.

A family of POTASSIUM and SODIUM-dependent acidic amino acid transporters that demonstrate a high affinity for GLUTAMIC ACID and ASPARTIC ACID. Several variants of this system are found in neuronal tissue.

A high-affinity, low capacity system y+ amino acid transporter with strong similarity to CATIONIC AMINO ACID TRANSPORTER 1. The two isoforms of the protein, CAT-2A and CAT-2B, exist due to alternative mRNA splicing. The transporter has specificity for the transport of ARGININE; LYSINE; and ORNITHINE.

Amino derivatives of caproic acid. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the amino caproic acid structure.

More From BioPortfolio on "Lysine Metabolic Availability in Children"

Quick Search

Relevant Topics

Pediatrics
Pediatrics is the general medicine of childhood. Because of the developmental processes (psychological and physical) of childhood, the involvement of parents, and the social management of conditions at home and at school, pediatrics is a specialty. With ...

Food
Food is any substance consumed to provide nutritional support for the body. It is usually of plant or animal origin, and contains essential nutrients, such as carbohydrates, fats, proteins, vitamins, or minerals. The substance is ingested by an organism ...


Searches Linking to this Trial