Topics

The Bacterial Secretome in IBD

2019-10-28 13:56:54 | BioPortfolio

Summary

Secreted bacterial effectors produced by the intestinal microbiota are in part responsible for the proinflammatory effect of the fecal content in inflammatory bowel disease (IBD) patients.

Description

Fecal colonic content will be collected by suction during routine colonoscopy. From the fecal content different fractions (containing soluble bacterial fractions and membrane bound bacterial fractions) will be processed. These bacterial fractions (also termed as bacterial secretome) will be tested for proinflammatory potential in tissue culture assays including epithelial colonic cell lines and dendritic/monocytic cell lines. The main outcome parameter will be the secretion of proinflammatory cytokines from the tissue culture.

For this purpose the investigators will test different groups of patients including patients with active/inactive Crohn's disease or ulcerative colitis, patients with other inflammatory conditions of the colon, patients with colorectal carcinoma and healthy controls undergoing colonoscopy.

Study Design

Conditions

Inflammatory Bowel Diseases

Intervention

Colonic fluid collection during endoscopy for analysis of the bacterial secretome

Location

Medical University of Graz
Graz
Austria

Status

Recruiting

Source

Medical University of Graz

Results (where available)

View Results

Links

Published on BioPortfolio: 2019-10-28T13:56:54-0400

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