Advertisement

Topics

An Investigation of Tumor Necrosis Factor (TNF)-Alpha in Asthma Using Biopsy Explants and Primary Bronchial Epithelial Cell Cultures

2014-08-27 03:12:16 | BioPortfolio

Summary

We have developed in vitro systems including primary epithelial cell cultures and explant cultures of bronchial tissues to study the interaction between the bronchial mucosa and allergens. This approach involves culturing bronchial biopsies under optimal conditions and stimulate them with allergens thus enabling us to perform a dynamic study without the need of performing several bronchoscopies and will allow the testing of unapproved substances, which could, otherwise, not be delivered, in vivo.

We wish to apply these ex vivo bronchial culture systems to assess cytokine release in moderately severe asthmatics and evaluate the effects of blocking TNF-alpha signalling using anti TNF-alpha monoclonal antibodies.

Description

Volunteers with moderately severe asthma will be recruited and carefully characterized in terms of lung function, asthma symptom scores, medication usage and allergen sensitivity (by blood and skin tests). If suitable, they will then be asked to withdraw their corticosteroids for a week with careful monitoring of the symptoms and peak flows before undergoing fibreoptic bronchoscopy as was carried out in the previous explant study. Bronchial brushings and biopsies will be carried out as per standard technique used in University medicine. Primary epithelial cell cultures will be established from bronchial brushings and will be used for optimising the dose of anti TNF-alpha required for inhibition of TNF-alpha induced responses. The biopsies will be placed in either culture medium alone, allergen extract or allergen extract plus anti TNF-alpha antibody and cultured for 24 hours. The biopsies will then be either snap frozen in liquid nitrogen for analysis of cytokines or processed for immunohistochemical analysis. The supernatants from each study will be recovered and analysed for cytokine (IL-5, IL-8, IL-13, MIP 1 alpha and RANTES) release by ELISA.

Study Design

Observational Model: Cohort, Time Perspective: Cross-Sectional

Conditions

Asthma

Location

Southampton University Hospital Trust
Southampton
Hampshire
United Kingdom
SO16 6YD

Status

Completed

Source

Southampton University Hospitals NHS Trust

Results (where available)

View Results

Links

Published on BioPortfolio: 2014-08-27T03:12:16-0400

Clinical Trials [1374 Associated Clinical Trials listed on BioPortfolio]

Impact of Adult Asthma

To assess the separate and interactive effects of asthma severity, subspecialty practice variation, asthma-related psychosocial variables, and other factors on asthma outcomes, including a...

Improving the Quality of Asthma Care Using the Internet

It is important for people with asthma to become involved in their asthma care and management. This study will evaluate an interactive Web site that provides tailored feedback and informat...

Asthma Inpatient Research on Education

Families of children with asthma are admitted for asthma attacks very frequently compared to adults with asthma. They are given education about asthma but studies are needed to improve thi...

Improving Asthma Communication in Minority Families

The purpose of this study is to determine if teaching children with asthma how to talk to their doctor about controlling their asthma including symptom frequency in an asthma diary and med...

Asthma Control, Quality of Life and Emotional Feelings in a Real Life Setting

This study aims to evaluate the asthma control status, asthma symptoms (severity, frequency, and limitations on activities, etc.), QOL, and use of drugs for asthma attack in adult asthma p...

PubMed Articles [1018 Associated PubMed Articles listed on BioPortfolio]

Update: Asthma Yardstick: Practical recommendations for a sustained step-up in asthma therapy for poorly controlled asthma.

Favorable response to asthma-dosed subcutaneous mepolizumab in eosinophilic pneumonia.

Mepolizumab targets eosinophils in the treatment of asthma. The dose used for asthma is considerably lower than that used for treating eosinophilic granulomatosis with polyangiitis, a recently approve...

Treatment patterns and related clinical consequences in adults with asthma.

A stepwise therapeutic management is recommended for asthma patients by the Global Initiative for Asthma (GINA). Little is known about the recommendations applied in real world settings. This study ai...

Seasonal changes influence the improvement in asthma symptoms by exercise training in subjects with asthma.

To investigate whether patients with moderate to severe asthma who commence an exercise training program in winter or summer show differences in exercise capacity, health-related quality of life (HRQo...

Characterisation of asthma in the adolescent population.

Asthma often debuts in early life (1). During childhood, the prevalence of asthma is higher in boys, but prevalence is higher in girls after puberty (2). The goal of asthma treatment is to achieve and...

Medical and Biotech [MESH] Definitions

Asthma attacks caused, triggered, or exacerbated by OCCUPATIONAL EXPOSURE.

Asthma attacks following a period of exercise. Usually the induced attack is short-lived and regresses spontaneously. The magnitude of postexertional airway obstruction is strongly influenced by the environment in which exercise is performed (i.e. inhalation of cold air during physical exertion markedly augments the severity of the airway obstruction; conversely, warm humid air blunts or abolishes it).

Drugs that are used to treat asthma.

A beta-adrenergic agonist used in the treatment of asthma and bronchospasms.

Adrenergic beta-2 agonist used as bronchodilator for emphysema, bronchitis and asthma.

More From BioPortfolio on "An Investigation of Tumor Necrosis Factor (TNF)-Alpha in Asthma Using Biopsy Explants and Primary Bronchial Epithelial Cell Cultures"

Advertisement
Quick Search
Advertisement
Advertisement

 

Relevant Topics

Cytokine Tumour Necrosis Factor (TNF)
TNF is a compound that is classified as a cytokine which plays a central role in the cellular mechanisms of apoptosis or cell death. However, there are a number of different kinds of TNF, just under twenty, but the family of molecules have very similar a...

Asthma
Asthma is caused by inflammation of small tubes, called bronchi, which carry air in and out of the lungs. If you have asthma, the bronchi will be inflamed and more sensitive than normal.  When you come into contact with something that irritates your...


Searches Linking to this Trial