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Storage Lesion in Banked Blood Due to Disruption of Nitric Oxide (NO) Homeostasis

2014-08-27 03:13:09 | BioPortfolio

Summary

To explore the impact of stored blood on blood vessel function by measuring the blood flow in the arm during Acetylcholine infusion and blood infusion in Normal Healthy volunteers.

Description

The increased storage time of transfused blood is associated with an increased risk of cardiovascular events and organ failure. The underlying biological mechanism as to why this happens is not understood. A major abnormality in aged blood is the reduced life span of red blood cells after they are infused. This is associated with rupture of the red blood cells and release of their contents. However, the degree of red blood cell rupture and release of the cell's contents in humans after transfusion has not been well studied. It has been seen that even low levels of red blood cell rupture severely decrease the amount of nitric oxide and other factors that effect how blood vessels function. The purpose of this study is to perform human forearm blood flow studies to evaluate wether there are a sufficient amount these factors released during red blood transfusion to significantly affect how blood vessel function in humans.

This study will enroll normal healthy volunteers between 18 to 50 years of age. 500 ml (1.0 unit) of blood will be collected from subjects who will then return in 5-10 days and be re-infused with the blood 5-10 days after storage and then return in 35-42 days and be re-infused with the blood 35-42 days after storage. The study will use a tool called strain gauge plethysmography and the drug acetylcholine to measure the effect of fresh (i.e., 5-10 days) versus aged (35-42 days) autologous blood transfusions on forearm blood flow in healthy volunteers.

Study Design

Allocation: Non-Randomized, Intervention Model: Single Group Assignment, Masking: Open Label, Primary Purpose: Basic Science

Conditions

Storage Lesion in Blood

Intervention

Intravenous Acetylcholine and blood transfusion

Location

University of Pittsburgh Medical Center
Pittsburgh
Pennsylvania
United States
15213

Status

Recruiting

Source

National Heart, Lung, and Blood Institute (NHLBI)

Results (where available)

View Results

Links

Published on BioPortfolio: 2014-08-27T03:13:09-0400

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Medical and Biotech [MESH] Definitions

The area of hematology that is concerned with the transfusion of blood and blood components, and in prevention and treatment of adverse effects from BLOOD TRANSFUSION errors.

The introduction of whole blood or blood component directly into the blood stream. (Dorland, 27th ed)

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In utero transfusion of BLOOD into the FETUS for the treatment of FETAL DISEASES, such as fetal erythroblastosis (ERYTHROBLASTOSIS, FETAL).

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