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Tissue Sectioning by Electro-Dissociation

2014-08-27 03:16:27 | BioPortfolio

Summary

Currently there is no technique to produce thin (0.004-0.01 mm) serial sections of large fresh tissue specimens that are suitable for high-resolution in situ protein/gene expression studies without ice artifact or fixation-induced molecular damage. Traditional frozen sectioning preserves protein and nucleic acid structure, but the inherent ice artifact precludes reconstruction of protein and mRNA expression patterns in 3-dimensions. Since the limitations of the existing sectioning techniques result from the fact that they rely on mechanical cutting which in turn require the tissue to be stiff, we suggest a new approach to cut tissue via an electro erosion process that utilizes focus radio frequency (RF).

Study Design

N/A

Conditions

Thin Sections

Intervention

Tissue Sectioning via Electro Erosion Process

Status

Terminated

Source

University of Arkansas

Results (where available)

View Results

Links

Published on BioPortfolio: 2014-08-27T03:16:27-0400

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Medical and Biotech [MESH] Definitions

The very long and thin extensions of telocytes' cell surface, that have alternating thick and thin sections called podoms and podomers.

The technique of placing cells or tissue in a supporting medium so that thin sections can be cut using a microtome. The medium can be paraffin wax (PARAFFIN EMBEDDING) or plastics (PLASTIC EMBEDDING) such as epoxy resins.

The technique of using a microtome to cut thin or ultrathin sections of tissues embedded in a supporting substance. The microtome is an instrument that hold a steel, glass or diamond knife in clamps at an angle to the blocks of prepared tissues, which it cuts in sections of equal thickness.

Thinly cut sections of frozen tissue specimens prepared with a cryostat or freezing microtome.

Loss of epithelial tissue from the surface of the cornea due to progressive erosion and necrosis of the tissue; usually caused by bacterial, fungal, or viral infection.

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