Topics

Tissue Sectioning by Electro-Dissociation

2014-08-27 03:16:27 | BioPortfolio

Summary

Currently there is no technique to produce thin (0.004-0.01 mm) serial sections of large fresh tissue specimens that are suitable for high-resolution in situ protein/gene expression studies without ice artifact or fixation-induced molecular damage. Traditional frozen sectioning preserves protein and nucleic acid structure, but the inherent ice artifact precludes reconstruction of protein and mRNA expression patterns in 3-dimensions. Since the limitations of the existing sectioning techniques result from the fact that they rely on mechanical cutting which in turn require the tissue to be stiff, we suggest a new approach to cut tissue via an electro erosion process that utilizes focus radio frequency (RF).

Study Design

N/A

Conditions

Thin Sections

Intervention

Tissue Sectioning via Electro Erosion Process

Status

Terminated

Source

University of Arkansas

Results (where available)

View Results

Links

Published on BioPortfolio: 2014-08-27T03:16:27-0400

Clinical Trials [908 Associated Clinical Trials listed on BioPortfolio]

Evaluate Marginal B. Loss in Immediate Implant Placement in Maxillary Esthetic Zone Using Socket Shield Versus Xenograft

Anterior maxilla (Esthetic zone) is very challenging area because of thin buccal plate of bone. After tooth extraction physiological process occur causing alteration of bone and soft tissu...

Evaluation of Tongue Base MucOsectomy & Step sErial Sectioning

Squamous cell carcinoma (SCC) is a cancer that originates from the cells lining the body and can spread into the lymph glands and beyond. Some patients first present with an SCC which has ...

Meta-analysis of Stannous Fluoride and the Effects on Enamel Erosion

The aim of this 5 study analysis was to compare the effect of bioavailable stannous fluoride (SnF2) dentifrices to assess enamel erosion protection in-situ using surface profilometry.

Clinical Evaluation of Tissue Sampling Bias in Histologic Evaluation of Sentinel Lymph Nodes in Breast Cancer

This is a study to evaluate the incidence of sampling bias during pathologic assessment of sentinel lymph nodes (SLN) when they are cut at a certain thickness (1 mm) and the tissue slices ...

Effect of Denosumab in Erosion Healing in RA

To evaluate the effects of denosumab on erosion healing and change in physical function in RA patients.

PubMed Articles [13681 Associated PubMed Articles listed on BioPortfolio]

Ultra-thin sectioning and grinding of epoxy plastinated tissue.

With classical sheet plastination techniques such as E12, the level and thickness of the freeze-cut sections decide on what is visible in the final sheet plastinated sections. However, there are other...

Comparison of four softening agents used on formalin-fixed paraffin-embedded nail biopsies with inflammatory disease.

Obtaining high-quality sections of the nail plate poses a significant challenge to histopathology technicians world over. Nail is a heavily keratotic hard tissue that tends to split or tear while sect...

Tissue expansion of lung bronchi due to tissue processing for histology - A comparative analysis of paraffin versus frozen sections in a pig model.

Tissue shrinking due to fixation and processing is well known. However, the degree of shrinking varies significantly with the tissue type as well as the processing method and is not well studied in va...

Analysis of depth-sectioning STEM for thick samples and 3D imaging.

We derive a model that describes 3D volume imaging in depth-sectioning STEM that is valid for all STEM techniques under three well-defined conditions: linearity, undisturbed probe and elastic scatteri...

In vitro characterisation of the erosion of soft tissues by surgical mesh.

Surgical mesh is used widely in operations to treat hernias, prolapsed organs, urinary incontinence, etc. A major complication following surgery is so-called "mesh erosion", in which the mesh material...

Medical and Biotech [MESH] Definitions

The very long and thin extensions of telocytes' cell surface, that have alternating thick and thin sections called podoms and podomers.

The technique of placing cells or tissue in a supporting medium so that thin sections can be cut using a microtome. The medium can be paraffin wax (PARAFFIN EMBEDDING) or plastics (PLASTIC EMBEDDING) such as epoxy resins.

The technique of using a microtome to cut thin or ultrathin sections of tissues embedded in a supporting substance. The microtome is an instrument that hold a steel, glass or diamond knife in clamps at an angle to the blocks of prepared tissues, which it cuts in sections of equal thickness.

Thinly cut sections of frozen tissue specimens prepared with a cryostat or freezing microtome.

Loss of epithelial tissue from the surface of the cornea due to progressive erosion and necrosis of the tissue; usually caused by bacterial, fungal, or viral infection.

More From BioPortfolio on "Tissue Sectioning by Electro-Dissociation"

Quick Search

Relevant Topics

Bioinformatics
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Nutrition
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...


Searches Linking to this Trial