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A New Enzymatic Assay for Rapid Diagnosing of Central Nervous System Enteroviral Infection

2014-08-27 03:23:31 | BioPortfolio

Summary

A new enzymatic assay was developed by NMD Diagnostics for rapid diagnosis of Enteroviral CNS infection. This study will compare this assay to RT-PCR, by testing human CSF samples taken from children at the ER who are undergoing a spinal tap due to suspicion of meningitis.

Description

MND's new enzymatic assay is based on the detection of a specific viral enzyme through the detection of its enzymatic reaction. The detection of a specific enzymatic reaction is indicative to the presence of the specific virus in a biological specimen. This study will compare this diagnostic assay to RT-PCR for diagnosis of enteroviral infection in cerebrospinal fluid. CSF samples will be taken from children undergoing a spinal tap at the pediatric ER due to a suspicion of meningitis. A total of 100 samples will be collected, from children 0-16 years old who's parents agreed on participation in the study. A comparison of the enzymatic assay to RT-PCR as well as calibration and validation of the new assay will be done with the harvested samples, for a rapid and accurate identification of Enterovirus in human cerebrospinal fluid.

Study Design

Observational Model: Case-Only, Time Perspective: Prospective

Conditions

Viral Meningitis

Location

Shaare Zedek Medical Center
Jerusalem
Israel
91031

Status

Active, not recruiting

Source

Shaare Zedek Medical Center

Results (where available)

View Results

Links

Published on BioPortfolio: 2014-08-27T03:23:31-0400

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