Advertisement

Topics

Cloning and expression of recombinant chondroitinase AC II and its comparison to the Arthrobacter aurescens enzyme

22:39 EDT 11 Aug 2017 | Wiley Biotechnology Journal

Chondroitin sulfates are the glycosaminoglycan chains of proteoglycans critical in the normal development and pathophysiology of all animals. Chondroitinase ACII, a polysaccharide lyase originally isolated from Arthrobacter aurescens IAM 110 65, which is widely used in the analysis and study of chondroitin structure, is no longer commercially available. The aim of the current study was to prepare recombinant versions of this critical enzyme for the glycobiology research community. Two versions of recombinant chondroitinase ACII were prepared in Escherichia coli, and their activity, stability, specificity and action pattern were examined, along with a non‐recombinant version secreted by an Arthrobacter strain. The recombinant enzymes were similar to the enzyme obtained from Arthrobacter for all examined properties, except for some subtle specificity differences towards uncommon chondroitin sulfate substrates. These differences are believed to be due to either post‐translational modification of the Arthrobacter‐secreted enzyme or other subtle structural differences between the recombinant and natural enzymes. The secreted chondroitinase can serve as a suitable replacement for the original enzyme that is currently unavailable, while the recombinant ones can be applied generally in the structural determination of most standard chondroitin sulfates.

Original Article: Cloning and expression of recombinant chondroitinase AC II and its comparison to the Arthrobacter aurescens enzyme

NEXT ARTICLE

More From BioPortfolio on "Cloning and expression of recombinant chondroitinase AC II and its comparison to the Arthrobacter aurescens enzyme"

Quick Search
Advertisement
 

Relevant Topics

Recombinant DNA
Recombinant DNA is the formation of a novel DNA sequence by the formation of two DNA strands. These are taken from two different organisms. These recombinant DNA molecules can be made with recombinant DNA technology. The procedure is to cut the DNA of ...

Enzymes
Enzymes are proteins that catalyze (i.e., increase the rates of) chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical re...