Homocysteine alters glutamate uptake and Na(+),K (+)-ATPase activity and oxidative status in rats hippocampus: protection by vitamin C.

06:51 EST 23rd November 2014 | BioPortfolio

Summary of "Homocysteine alters glutamate uptake and Na(+),K (+)-ATPase activity and oxidative status in rats hippocampus: protection by vitamin C."

In the present study we investigate the effect of homocysteine on glutamate uptake, Na(+),K(+)-ATPase, enzymatic antioxidant defenses, as well as reactive species levels in hippocampus of rats. The influence of vitamin C, a classic antioxidant, on the effects elicited by homocysteine was also tested. Results showed that chronic hyperhomocysteinemia decreased glutamate uptake and the activities of Na(+),K(+)-ATPase, catalase and superoxide dismutase in hippocampus of rats. Reactive species levels were increased by chronic homocysteine administration. Concomitant administration of vitamin C significantly prevented these alterations caused by homocysteine. According to our results, it seems possible to suggest that the reduction in glutamate uptake and Na(+),K(+)-ATPase activity may be mediated by oxidative stress, since vitamin C prevented these effects. We suggest that the administration of antioxidants should be considered as an adjuvant therapy to specific diet in homocystinuria.

Affiliation

Laboratório de Neuroproteção e Doença Metabólica, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil.

Journal Details

This article was published in the following journal.

Name: Metabolic brain disease
ISSN: 1573-7365
Pages:

Links

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Correction: Role of Na,K-ATPase α1 and α2 Isoforms in the Support of Astrocyte Glutamate Uptake.

[This corrects the article DOI: 10.1371/journal.pone.0098469.].

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